Cytotoxicity and inhibition of P-glycoprotein by selected medicinal plants from Thailand
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Chanai Noysang
- Anne Mahringer
- Maen Zeino
- Mohamed Saeed
- Omboon Luanratana
- Gert Fricker
- Rudolf Bauer
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000340854000063&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.jep.2014.06.001
- eISSN
- 1872-7573
- Externe Identifier
- Clarivate Analytics Document Solution ID: AN8KV
- PubMed Identifier: 24929106
- ISSN
- 0378-8741
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- JOURNAL OF ETHNOPHARMACOLOGY
- Schlüsselwörter
- ABC transporter
- Blood-brain barrier
- Chemotherapy
- Herbal therapy
- Multidrug resistance
- Natural products
- Paginierung
- 633 - 641
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Titel
- Cytotoxicity and inhibition of P-glycoprotein by selected medicinal plants from Thailand
- Sub types
- Article
- Ausgabe der Zeitschrift
- 155
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Chanai Noysang
- Anne Mahringer
- Maen Zeino
- Mohamed Saeed
- Omboon Luanratana
- Gert Fricker
- Rudolf Bauer
- Thomas Efferth
- DOI
- 10.1016/j.jep.2014.06.001
- ISSN
- 0378-8741
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Journal of Ethnopharmacology
- Sprache
- en
- Paginierung
- 633 - 641
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.jep.2014.06.001
- Datum der Datenerfassung
- 2019
- Titel
- Cytotoxicity and inhibition of P-glycoprotein by selected medicinal plants from Thailand
- Ausgabe der Zeitschrift
- 155
Data source: Crossref
- Abstract
- <h4>Ethnopharmacological relevance</h4>Thai medicine has a long tradition of tonifying medicinal plants. In the present investigation, we studied the flower extracts of Jasminum sambac, Mammea siamensis, Mesua ferrea, Michelia alba, Mimusops elengi, and Nelumbo nucifera and speculated that these plants might influence metabolism and substance flow in the body.<h4>Materials and methods</h4>Isolation of porcine brain capillary endothelial cells (PBCECs) as well as multidrug-resistance CEM/ADR5000 leukemia cells, MDA-M;B-231 breast cancer, U-251 brain tumor, and HCT-116 colon cancer cells were used. The calcein-acetoxymethylester (AM) assay was used to measure inhibition of P-glycoprotein transport. XTT and resazurin assays served for measuring cytotoxicity.<h4>Results</h4>The extracts revealed cytotoxicity towards CCRF-CEM leukemia cells to a different extent. The strongest growth inhibition was found for the n-hexane extracts of Mammea siamensis and Mesua ferrea, and the dichloromethane extracts of Mesua ferrea and Michelia alba. The flower extracts also inhibited P-glycoprotein function in porcine brain capillary endothelial cells and CEM/ADR5000 leukemia cells, indicating modulation of the blood-brain barrier and multidrug resistance of tumors. Bioactivity-guided isolation of coumarins from Mammea siamensis flowers revealed considerable cytotoxicity of mammea A/AA, deacetylmammea E/BA and deacetylmammea E/BB towards human MDA-MB-231 breast cancer, U-251 brain tumor, HCT-116 colon cancer, and CCRF-CEM leukemia cells.<h4>Conclusion</h4>The plants analyzed may be valuable in developing novel treatment strategies to overcome the blood-brain barrier and multidrug-resistance in tumor cells mediated by P-glycoprotein.
- Addresses
- Institute of Pharmaceutical Sciences, Department of Pharmacognosy, Karl-Franzens-University Graz, Graz, Austria; Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand; Thai Traditional Medicine College, Rajamangala University of Technology Thayaburi, Phathumthani, Thailand.
- Autoren
- Chanai Noysang
- Anne Mahringer
- Maen Zeino
- Mohamed Saeed
- Omboon Luanratana
- Gert Fricker
- Rudolf Bauer
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1016/j.jep.2014.06.001
- eISSN
- 1872-7573
- Externe Identifier
- PubMed Identifier: 24929106
- Open access
- false
- ISSN
- 0378-8741
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Journal of ethnopharmacology
- Schlüsselwörter
- Blood-Brain Barrier
- Endothelium, Vascular
- Brain
- Cell Line, Tumor
- Endothelial Cells
- Animals
- Swine
- Humans
- Flowers
- Plants, Medicinal
- Neoplasms
- Plant Extracts
- Antineoplastic Agents, Phytogenic
- Drug Resistance, Multiple
- Drug Resistance, Neoplasm
- Thailand
- Medicine, East Asian Traditional
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2014
- Paginierung
- 633 - 641
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Datum der Datenerfassung
- 2014
- Titel
- Cytotoxicity and inhibition of P-glycoprotein by selected medicinal plants from Thailand.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 155
Data source: Europe PubMed Central
- Abstract
- ETHNOPHARMACOLOGICAL RELEVANCE: Thai medicine has a long tradition of tonifying medicinal plants. In the present investigation, we studied the flower extracts of Jasminum sambac, Mammea siamensis, Mesua ferrea, Michelia alba, Mimusops elengi, and Nelumbo nucifera and speculated that these plants might influence metabolism and substance flow in the body. MATERIALS AND METHODS: Isolation of porcine brain capillary endothelial cells (PBCECs) as well as multidrug-resistance CEM/ADR5000 leukemia cells, MDA-M;B-231 breast cancer, U-251 brain tumor, and HCT-116 colon cancer cells were used. The calcein-acetoxymethylester (AM) assay was used to measure inhibition of P-glycoprotein transport. XTT and resazurin assays served for measuring cytotoxicity. RESULTS: The extracts revealed cytotoxicity towards CCRF-CEM leukemia cells to a different extent. The strongest growth inhibition was found for the n-hexane extracts of Mammea siamensis and Mesua ferrea, and the dichloromethane extracts of Mesua ferrea and Michelia alba. The flower extracts also inhibited P-glycoprotein function in porcine brain capillary endothelial cells and CEM/ADR5000 leukemia cells, indicating modulation of the blood-brain barrier and multidrug resistance of tumors. Bioactivity-guided isolation of coumarins from Mammea siamensis flowers revealed considerable cytotoxicity of mammea A/AA, deacetylmammea E/BA and deacetylmammea E/BB towards human MDA-MB-231 breast cancer, U-251 brain tumor, HCT-116 colon cancer, and CCRF-CEM leukemia cells. CONCLUSION: The plants analyzed may be valuable in developing novel treatment strategies to overcome the blood-brain barrier and multidrug-resistance in tumor cells mediated by P-glycoprotein.
- Date of acceptance
- 2014
- Autoren
- Chanai Noysang
- Anne Mahringer
- Maen Zeino
- Mohamed Saeed
- Omboon Luanratana
- Gert Fricker
- Rudolf Bauer
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/24929106
- DOI
- 10.1016/j.jep.2014.06.001
- eISSN
- 1872-7573
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- J Ethnopharmacol
- Schlüsselwörter
- ABC transporter
- Blood–brain barrier
- Chemotherapy
- Herbal therapy
- Multidrug resistance
- Natural products
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Animals
- Antineoplastic Agents, Phytogenic
- Blood-Brain Barrier
- Brain
- Cell Line, Tumor
- Drug Resistance, Multiple
- Drug Resistance, Neoplasm
- Endothelial Cells
- Endothelium, Vascular
- Flowers
- Humans
- Medicine, East Asian Traditional
- Neoplasms
- Plant Extracts
- Plants, Medicinal
- Swine
- Thailand
- Sprache
- eng
- Country
- Ireland
- Paginierung
- 633 - 641
- PII
- S0378-8741(14)00435-8
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2015
- Titel
- Cytotoxicity and inhibition of P-glycoprotein by selected medicinal plants from Thailand.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 155
Data source: PubMed
- Beziehungen:
- Property of