Cytotoxicity of four Aframomum species (A. arundinaceum, A. alboviolaceum, A. kayserianum and A. polyanthum) towards multi-factorial drug resistant cancer cell lines
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Victor Kuete
- Patrick Y Ango
- Samuel O Yeboah
- Armelle T Mbaveng
- Renameditswe Mapitse
- Gilbert DWF Kapche
- Bonaventure T Ngadjui
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000342105400001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1186/1472-6882-14-340
- eISSN
- 1472-6882
- Externe Identifier
- Clarivate Analytics Document Solution ID: AP5FP
- PubMed Identifier: 25239700
- Zeitschrift
- BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
- Schlüsselwörter
- Aframomum
- Cameroon
- Cancer
- Cytotoxicity
- Multidrug resistant
- Zingiberaceae
- Artikelnummer
- ARTN 340
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Titel
- Cytotoxicity of four <i>Aframomum</i> species (<i>A</i>. <i>arundinaceum</i>, <i>A</i>. <i>alboviolaceum</i>, <i>A</i>. <i>kayserianum</i> and <i>A</i>. <i>polyanthum</i>) towards multi-factorial drug resistant cancer cell lines
- Sub types
- Article
- Ausgabe der Zeitschrift
- 14
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Victor Kuete
- Patrick Y Ango
- Samuel O Yeboah
- Armelle T Mbaveng
- Renameditswe Mapitse
- Gilbert DWF Kapche
- Bonaventure T Ngadjui
- Thomas Efferth
- DOI
- 10.1186/1472-6882-14-340
- eISSN
- 1472-6882
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- BMC Complementary and Alternative Medicine
- Sprache
- en
- Artikelnummer
- 340
- Online publication date
- 2014
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1186/1472-6882-14-340
- Datum der Datenerfassung
- 2019
- Titel
- Cytotoxicity of four Aframomum species (A. arundinaceum, A. alboviolaceum, A. kayserianum and A. polyanthum) towards multi-factorial drug resistant cancer cell lines
- Ausgabe der Zeitschrift
- 14
Data source: Crossref
- Abstract
- <h4>Background</h4>The search for natural products as potential cytotoxic agents has yielded promising candidates. However multidrug resistance (MDR) is still a major hurdle for patients receiving chemotherapy. In the present study, we evaluated the cytotoxicity of the methanol extracts of four dietary Aframomum plant species (A. arundinaceum, A. alboviolaceum, A. kayserianum and A. polyanthum) against nine sensitive and MDR cancer cell lines. We have also identified the bioactive constituents of A. arundinaceum.<h4>Methods</h4>The cytotoxicity of the methanol extracts of the above plants was determined using a resazurin reduction assay. Chromatographic techniques were used to isolate the constituents of A. arundinaceum.<h4>Results</h4>A preliminary experiment on leukemia CCRF-CEM cells at 40 μg/mL showed that the extracts from A. kayserianum and A. alboviolaceum as well as the isolated compounds namely compounds aframodial (1), 8(17),12-labdadien-15,16-dial (2), galanolactone (3), 1-p-menthene-3,6-diol (6) and 1,4-dimethoxybenzene (7) were less active, inducing more than 50% growth of this cell line contrary to A. polyanthum and A. arundinaceum extracts, galanals A (4) and B (5), naringenin (8) and kaempferol-3,7,4'-trimethylether (9). The IC50 values below or around 30 μg/mL were recorded with A. arundinaceum extract against eight of the nine tested cancer cell lines. This extract as well as compound 8 displayed IC50 values below 40 μg/mL towards the nine tested cancer cell lines whilst A. polyanthum extract, compounds 4, 5 and 9 showed selective activities. Collateral sensitivity (hypersensitivity) was observed with A. arundinaceum extract towards leukemia CEM/ADR5000 cells and glioblastoma U87MG.ΔEGFR compared to their respective sensitive counterparts CEM/CEM and U87MG.<h4>Conclusion</h4>The results of this study provide evidence of the cytotoxicity selected Aframomum species as well as a baseline information for the potential use of Aframomum arundinaceum in the fight against drug sensitive and otherwise drug-resistant cancers.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, 55128 Mainz, Germany. kuetevictor@yahoo.fr.
- Autoren
- Victor Kuete
- Patrick Y Ango
- Samuel O Yeboah
- Armelle T Mbaveng
- Renameditswe Mapitse
- Gilbert DWF Kapche
- Bonaventure T Ngadjui
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1186/1472-6882-14-340
- eISSN
- 1472-6882
- Externe Identifier
- PubMed Identifier: 25239700
- PubMed Central ID: PMC4177760
- Open access
- true
- ISSN
- 1472-6882
- Zeitschrift
- BMC complementary and alternative medicine
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Zingiberaceae
- Plant Extracts
- Antineoplastic Agents
- Cell Proliferation
- Cell Survival
- Drug Resistance, Neoplasm
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2014
- Open access status
- Open Access
- Paginierung
- 340
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2014
- Titel
- Cytotoxicity of four Aframomum species (A. arundinaceum, A. alboviolaceum, A. kayserianum and A. polyanthum) towards multi-factorial drug resistant cancer cell lines.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 14
Files
https://bmccomplementmedtherapies.biomedcentral.com/counter/pdf/10.1186/1472-6882-14-340 https://europepmc.org/articles/PMC4177760?pdf=render
Data source: Europe PubMed Central
- Abstract
- BACKGROUND: The search for natural products as potential cytotoxic agents has yielded promising candidates. However multidrug resistance (MDR) is still a major hurdle for patients receiving chemotherapy. In the present study, we evaluated the cytotoxicity of the methanol extracts of four dietary Aframomum plant species (A. arundinaceum, A. alboviolaceum, A. kayserianum and A. polyanthum) against nine sensitive and MDR cancer cell lines. We have also identified the bioactive constituents of A. arundinaceum. METHODS: The cytotoxicity of the methanol extracts of the above plants was determined using a resazurin reduction assay. Chromatographic techniques were used to isolate the constituents of A. arundinaceum. RESULTS: A preliminary experiment on leukemia CCRF-CEM cells at 40 μg/mL showed that the extracts from A. kayserianum and A. alboviolaceum as well as the isolated compounds namely compounds aframodial (1), 8(17),12-labdadien-15,16-dial (2), galanolactone (3), 1-p-menthene-3,6-diol (6) and 1,4-dimethoxybenzene (7) were less active, inducing more than 50% growth of this cell line contrary to A. polyanthum and A. arundinaceum extracts, galanals A (4) and B (5), naringenin (8) and kaempferol-3,7,4'-trimethylether (9). The IC50 values below or around 30 μg/mL were recorded with A. arundinaceum extract against eight of the nine tested cancer cell lines. This extract as well as compound 8 displayed IC50 values below 40 μg/mL towards the nine tested cancer cell lines whilst A. polyanthum extract, compounds 4, 5 and 9 showed selective activities. Collateral sensitivity (hypersensitivity) was observed with A. arundinaceum extract towards leukemia CEM/ADR5000 cells and glioblastoma U87MG.ΔEGFR compared to their respective sensitive counterparts CEM/CEM and U87MG. CONCLUSION: The results of this study provide evidence of the cytotoxicity selected Aframomum species as well as a baseline information for the potential use of Aframomum arundinaceum in the fight against drug sensitive and otherwise drug-resistant cancers.
- Date of acceptance
- 2014
- Autoren
- Victor Kuete
- Patrick Y Ango
- Samuel O Yeboah
- Armelle T Mbaveng
- Renameditswe Mapitse
- Gilbert DWF Kapche
- Bonaventure T Ngadjui
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/25239700
- DOI
- 10.1186/1472-6882-14-340
- eISSN
- 1472-6882
- Externe Identifier
- PubMed Central ID: PMC4177760
- Zeitschrift
- BMC Complement Altern Med
- Schlüsselwörter
- Antineoplastic Agents
- Cell Line, Tumor
- Cell Proliferation
- Cell Survival
- Drug Resistance, Neoplasm
- Humans
- Plant Extracts
- Zingiberaceae
- Sprache
- eng
- Country
- England
- Paginierung
- 340
- PII
- 1472-6882-14-340
- Datum der Veröffentlichung
- 2014
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2014
- Titel
- Cytotoxicity of four Aframomum species (A. arundinaceum, A. alboviolaceum, A. kayserianum and A. polyanthum) towards multi-factorial drug resistant cancer cell lines.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 14
Data source: PubMed
- Beziehungen:
- Property of