Phytochemical profile and bioactivity of traditional ayurvedic decoctions and hydro-alcoholic macerations of Boerhaavia diffusa L. and Curculigo orchioides Gaertn.

Autoren

Massimo Tacchini
Antonella Spagnoletti
Matteo Marieschi
Augusta Caligiani
Renato Bruni
Thomas Efferth
Gianni Sacchetti
Alessandra Guerrini

DOI

10.1080/14786419.2014.1003299

eISSN

1478-6427

ISSN

1478-6419

Ausgabe der Veröffentlichung

22

Zeitschrift

Natural Product Research

Sprache

en

Online publication date

2015

Paginierung

2071 - 2079

Datum der Veröffentlichung

2015

Status

Published

Herausgeber

Informa UK Limited

Herausgeber URL

http://dx.doi.org/10.1080/14786419.2014.1003299

Datum der Datenerfassung

2019

Titel

Phytochemical profile and bioactivity of traditional ayurvedic decoctions and hydro-alcoholic macerations of<i>Boerhaavia diffusa</i>L. and<i>Curculigo orchioides</i>Gaertn.

Ausgabe der Zeitschrift

29

Data source: Crossref

Abstract

Decoctions (DECs) and hydro-alcoholic extracts (HEs) prepared from roots of Boerhaavia diffusa L. (Nyctaginaceae) and Curculigo orchioides Gaertn. (Hypoxidaceae) were phytochemically characterised by HPLC-DAD and profiled for their antioxidant, antigenotoxic and cytotoxic activities. B. diffusa DEC was rich in ferulic acid and vanillin, while the HE also contained boeravinone B and eupalitin. Both C. orchioides HE and DEC displayed the main occurrence of orcinol-β-d-glucoside and curculigoside A. Antioxidant activity was assayed through spectrophotometric DPPH, ABTS and β-carotene bleaching test, and using (HP)TLC bioautographic strategies. For both crude drugs, HE was the best performing preparation. Properly modified SOS-Chromotest evidenced a 10% inhibition by phytocomplexes against 4-nitroquinoline-N-oxide, and a higher bioactivity for vanillin (36.60 ± 1.68%) and ferulic acid (35.09 ± 1.53%). C. orchioides HE was the preparation which showed higher cytotoxicity against drug-sensitive human T-lymphoblastoid cell line (CCRF-CEM) and multidrug-resistant leukaemia cell line (CEM/ADR5000), and eupalitin was the only pure compound to exhibit an IC50 value.

Addresses

a Department of Life Sciences and Biotechnology (SVeB) , University of Ferrara , C.so Ercole I d'Este 32, 44121 Ferrara , Italy.

Autoren

Massimo Tacchini
Antonella Spagnoletti
Matteo Marieschi
Augusta Caligiani
Renato Bruni
Thomas Efferth
Thomas Efferth
Gianni Sacchetti
Alessandra Guerrini

DOI

10.1080/14786419.2014.1003299

eISSN

1478-6427

Externe Identifier

PubMed Identifier: 25612143

Open access

false

ISSN

1478-6419

Ausgabe der Veröffentlichung

22

Zeitschrift

Natural product research

Schlüsselwörter

Cell Line, Tumor
Humans
Curculigo
Nyctaginaceae
Plant Roots
Phenols
Plant Extracts
Antineoplastic Agents, Phytogenic
Antioxidants
Medicine, Ayurvedic
Phytochemicals

Sprache

eng

Medium

Print-Electronic

Online publication date

2015

Paginierung

2071 - 2079

Datum der Veröffentlichung

2015

Status

Published

Datum der Datenerfassung

2015

Titel

Phytochemical profile and bioactivity of traditional ayurvedic decoctions and hydro-alcoholic macerations of Boerhaavia diffusa L. and Curculigo orchioides Gaertn.

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

29

Data source: Europe PubMed Central

Abstract

Decoctions (DECs) and hydro-alcoholic extracts (HEs) prepared from roots of Boerhaavia diffusa L. (Nyctaginaceae) and Curculigo orchioides Gaertn. (Hypoxidaceae) were phytochemically characterised by HPLC-DAD and profiled for their antioxidant, antigenotoxic and cytotoxic activities. B. diffusa DEC was rich in ferulic acid and vanillin, while the HE also contained boeravinone B and eupalitin. Both C. orchioides HE and DEC displayed the main occurrence of orcinol-β-d-glucoside and curculigoside A. Antioxidant activity was assayed through spectrophotometric DPPH, ABTS and β-carotene bleaching test, and using (HP)TLC bioautographic strategies. For both crude drugs, HE was the best performing preparation. Properly modified SOS-Chromotest evidenced a 10% inhibition by phytocomplexes against 4-nitroquinoline-N-oxide, and a higher bioactivity for vanillin (36.60 ± 1.68%) and ferulic acid (35.09 ± 1.53%). C. orchioides HE was the preparation which showed higher cytotoxicity against drug-sensitive human T-lymphoblastoid cell line (CCRF-CEM) and multidrug-resistant leukaemia cell line (CEM/ADR5000), and eupalitin was the only pure compound to exhibit an IC50 value.

Autoren

Massimo Tacchini
Antonella Spagnoletti
Matteo Marieschi
Augusta Caligiani
Renato Bruni
Thomas Efferth
Gianni Sacchetti
Alessandra Guerrini

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/25612143

DOI

10.1080/14786419.2014.1003299

eISSN

1478-6427

Ausgabe der Veröffentlichung

22

Zeitschrift

Nat Prod Res

Schlüsselwörter

Boerhaavia diffusa
Curculigo orchioides
antioxidant activity
chemical fingerprinting
cytotoxicity
genotoxicity
traditional preparations
Antineoplastic Agents, Phytogenic
Antioxidants
Cell Line, Tumor
Curculigo
Humans
Medicine, Ayurvedic
Nyctaginaceae
Phenols
Phytochemicals
Plant Extracts
Plant Roots

Sprache

eng

Country

England

Paginierung

2071 - 2079

Datum der Veröffentlichung

2015

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2015

Titel

Phytochemical profile and bioactivity of traditional ayurvedic decoctions and hydro-alcoholic macerations of Boerhaavia diffusa L. and Curculigo orchioides Gaertn.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

29

Data source: PubMed

Phytochemical profile and bioactivity of traditional ayurvedic decoctions and hydro-alcoholic macerations of Boerhaavia diffusa L. and Curculigo orchioides Gaertn.

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