Laurus nobilis L. Seed Extract Reveals Collateral Sensitivity in Multidrug-Resistant P-Glycoprotein-Expressing Tumor Cells
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Antoine M Saab
- Alessandra Guerrini
- Maen Zeino
- Benjamin Wiench
- Damiano Rossi
- Roberto Gambari
- Gianni Sacchetti
- Henry Johannes Greten
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000353771000013&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1080/01635581.2015.1019632
- eISSN
- 1532-7914
- Externe Identifier
- Clarivate Analytics Document Solution ID: CH1HC
- PubMed Identifier: 25837780
- ISSN
- 0163-5581
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL
- Paginierung
- 664 - 675
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Titel
- <i>Laurus nobilis</i> L. Seed Extract Reveals Collateral Sensitivity in Multidrug-Resistant P-Glycoprotein-Expressing Tumor Cells
- Sub types
- Article
- Ausgabe der Zeitschrift
- 67
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Antoine M Saab
- Alessandra Guerrini
- Maen Zeino
- Benjamin Wiench
- Damiano Rossi
- Roberto Gambari
- Gianni Sacchetti
- Henry Johannes Greten
- Thomas Efferth
- DOI
- 10.1080/01635581.2015.1019632
- eISSN
- 1532-7914
- ISSN
- 0163-5581
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Nutrition and Cancer
- Sprache
- en
- Online publication date
- 2015
- Paginierung
- 664 - 675
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Herausgeber
- Informa UK Limited
- Herausgeber URL
- http://dx.doi.org/10.1080/01635581.2015.1019632
- Datum der Datenerfassung
- 2016
- Titel
- <i>Laurus nobilis</i>L. Seed Extract Reveals Collateral Sensitivity in Multidrug-Resistant P-Glycoprotein-Expressing Tumor Cells
- Ausgabe der Zeitschrift
- 67
Data source: Crossref
- Abstract
- The frequent failure of standard cancer chemotherapy requires the development of novel drugs capable of killing otherwise drug-resistant tumors. Here, we have investigated a chloroform extract of Laurus nobilis seeds. Fatty acids and 23 constituents of the volatile fraction were identified by gas chromotography/flame ionization detection (GC/FID) and gas chromatography/mass spectrometry (GC/MS), in good agreement with (1)H NMR (nuclear magnetic resonance) spectrum. Multidrug-resistant P-glycoprotein-expressing CEM/ADR5000 leukemia cells were hypersensitive (collaterally sensitive) toward this extract compared to drug-sensitive CCRF-CEM cells, whereas CEM/ADR5000 cells were 2586-fold resistant to doxorubicin as control drug. Collateral sensitivity was verified by measurement of apoptotic cells by flow cytometry. The log10IC50 values of 3 compounds in the extract (limonene, eucalyptol, oleic acid) did not correlate with mRNA expression of the P-glycoprotein-coding ABCB1/MDR1 gene and accumulation of the P-glycoprotein substrate rhodamine in the NCI panel of tumor cell lines. A microarray-based profile of 20 genes predicted resistance to doxorubicin and 7 other anticancer drugs involved in the multidrug resistance phenotype but not to limonene, eucalyptol and oleic acid. In conclusion, our results show that Laurus nobilis seed extract is suitable to kill multidrug-resistant P-glycoprotein expressing tumor cells.
- Addresses
- a Faculty of Sciences II , Lebanese University , Chemistry Department , Beirut , Lebanon.
- Autoren
- Antoine M Saab
- Alessandra Guerrini
- Maen Zeino
- Benjamin Wiench
- Damiano Rossi
- Roberto Gambari
- Gianni Sacchetti
- Henry Johannes Greten
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1080/01635581.2015.1019632
- eISSN
- 1532-7914
- Externe Identifier
- PubMed Identifier: 25837780
- Open access
- false
- ISSN
- 0163-5581
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Nutrition and cancer
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Laurus
- Seeds
- Leukemia
- Doxorubicin
- Plant Extracts
- Antineoplastic Agents, Phytogenic
- Cell Survival
- Drug Resistance, Neoplasm
- Gas Chromatography-Mass Spectrometry
- ATP Binding Cassette Transporter, Subfamily B
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2015
- Paginierung
- 664 - 675
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Datum der Datenerfassung
- 2015
- Titel
- Laurus nobilis L. Seed Extract Reveals Collateral Sensitivity in Multidrug-Resistant P-Glycoprotein-Expressing Tumor Cells.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 67
Data source: Europe PubMed Central
- Abstract
- The frequent failure of standard cancer chemotherapy requires the development of novel drugs capable of killing otherwise drug-resistant tumors. Here, we have investigated a chloroform extract of Laurus nobilis seeds. Fatty acids and 23 constituents of the volatile fraction were identified by gas chromotography/flame ionization detection (GC/FID) and gas chromatography/mass spectrometry (GC/MS), in good agreement with (1)H NMR (nuclear magnetic resonance) spectrum. Multidrug-resistant P-glycoprotein-expressing CEM/ADR5000 leukemia cells were hypersensitive (collaterally sensitive) toward this extract compared to drug-sensitive CCRF-CEM cells, whereas CEM/ADR5000 cells were 2586-fold resistant to doxorubicin as control drug. Collateral sensitivity was verified by measurement of apoptotic cells by flow cytometry. The log10IC50 values of 3 compounds in the extract (limonene, eucalyptol, oleic acid) did not correlate with mRNA expression of the P-glycoprotein-coding ABCB1/MDR1 gene and accumulation of the P-glycoprotein substrate rhodamine in the NCI panel of tumor cell lines. A microarray-based profile of 20 genes predicted resistance to doxorubicin and 7 other anticancer drugs involved in the multidrug resistance phenotype but not to limonene, eucalyptol and oleic acid. In conclusion, our results show that Laurus nobilis seed extract is suitable to kill multidrug-resistant P-glycoprotein expressing tumor cells.
- Autoren
- Antoine M Saab
- Alessandra Guerrini
- Maen Zeino
- Benjamin Wiench
- Damiano Rossi
- Roberto Gambari
- Gianni Sacchetti
- Henry Johannes Greten
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/25837780
- DOI
- 10.1080/01635581.2015.1019632
- eISSN
- 1532-7914
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Nutr Cancer
- Schlüsselwörter
- ATP Binding Cassette Transporter, Subfamily B
- Antineoplastic Agents, Phytogenic
- Cell Line, Tumor
- Cell Survival
- Doxorubicin
- Drug Resistance, Neoplasm
- Gas Chromatography-Mass Spectrometry
- Humans
- Laurus
- Leukemia
- Plant Extracts
- Seeds
- Sprache
- eng
- Country
- United States
- Paginierung
- 664 - 675
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2016
- Titel
- Laurus nobilis L. Seed Extract Reveals Collateral Sensitivity in Multidrug-Resistant P-Glycoprotein-Expressing Tumor Cells.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 67
Data source: PubMed
- Beziehungen:
- Property of