Cytotoxicity of natural products and derivatives toward MCF-7 cell monolayers and cancer stem-like mammospheres

Publication type:
Journal article
Metadata:
Autoren
  • Ean-Jeong Seo
  • Benjamin Wiench
  • Rebecca Hamm
  • Malte Paulsen
  • Yuangang Zu
  • Yujie Fu
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000353556300003&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.phymed.2015.01.012
eISSN
1618-095X
Externe Identifier
  • Clarivate Analytics Document Solution ID: CG8KL
  • PubMed Identifier: 25925965
ISSN
0944-7113
Ausgabe der Veröffentlichung
4
Zeitschrift
PHYTOMEDICINE
Schlüsselwörter
  • Drug resistance
  • Mammospheres
  • Cancer slem-like cells
  • Cajanin stilbene acid (CSA)
  • Shikoriin
  • Artesunate
Paginierung
438 - 443
Datum der Veröffentlichung
2015
Status
Published
Titel
Cytotoxicity of natural products and derivatives toward MCF-7 cell monolayers and cancer stem-like mammospheres
Sub types
  • Article
Ausgabe der Zeitschrift
22

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Ean-Jeong Seo
  • Benjamin Wiench
  • Rebecca Hamm
  • Malte Paulsen
  • Yuangang Zu
  • Yujie Fu
  • Thomas Efferth
DOI
10.1016/j.phymed.2015.01.012
ISSN
0944-7113
Ausgabe der Veröffentlichung
4
Zeitschrift
Phytomedicine
Sprache
en
Paginierung
438 - 443
Datum der Veröffentlichung
2015
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.phymed.2015.01.012
Datum der Datenerfassung
2018
Titel
Cytotoxicity of natural products and derivatives toward MCF-7 cell monolayers and cancer stem-like mammospheres
Ausgabe der Zeitschrift
22

Data source: Crossref

Abstract
<h4>Unlabelled</h4>Although cancer stem-like cells (CSCs) are rare, they can enter a non-proliferative or dormant state and resist therapy. Furthermore, quiescent CSCs are responsible for metastases that can appear after curative surgical treatment of a primary tumor. Because of drug resistance of CSCs, the development of novel therapies is urgently required that specifically target CSCs.<h4>Purpose</h4>The aim of the present study was to investigate the potential of a panel of natural products and derivatives to inhibit CSC-enriched mammospheres of MCF-7 breast cancer cells.<h4>Methods</h4>CD44(high)/CD24(low) cells were identified by flow cytometry and maintained as mammospheres. As a control, we used two clinically established anticancer drugs (5-fluorouracil and docetaxel). A panel of natural products, shikonin, two cajanin stilbene acid (CSA) derivatives and artesunate were tested by resazurin assay on CSC-enriched mammospheres and MCF-7 monolayer cells. Besides, cellular shikonin uptake experiments were performed by flow cytometry.<h4>Results</h4>We found two CSA derivatives (Nos. 6 and 19) to be active cancer stem-like MCF-7 mammospheres. Especially, CSA derivative No. 19 clearly showed collateral sensitivity in mammospheres compared to monolayer cells.<h4>Conclusion</h4>Phytochemicals which provoke collateral sensitivity in cancer-stem like cells are worth more detailed investigations in the future, since there is a great potential for improved chemotherapy to eradicate tumors and prolong cancer patients' survival times.
Addresses
  • Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Staudinger Weg 5, 55128 Mainz, Germany.
Autoren
  • Ean-Jeong Seo
  • Benjamin Wiench
  • Rebecca Hamm
  • Malte Paulsen
  • Yuangang Zu
  • Yujie Fu
  • Thomas Efferth
  • Thomas Efferth
DOI
10.1016/j.phymed.2015.01.012
eISSN
1618-095X
Externe Identifier
  • PubMed Identifier: 25925965
Open access
false
ISSN
0944-7113
Ausgabe der Veröffentlichung
4
Zeitschrift
Phytomedicine : international journal of phytotherapy and phytopharmacology
Schlüsselwörter
  • Spheroids, Cellular
  • Humans
  • Salicylates
  • Stilbenes
  • Artemisinins
  • Naphthoquinones
  • Antineoplastic Agents
  • Neoplastic Stem Cells
  • MCF-7 Cells
  • Phytochemicals
  • Artesunate
Sprache
eng
Medium
Print-Electronic
Online publication date
2015
Paginierung
438 - 443
Datum der Veröffentlichung
2015
Status
Published
Datum der Datenerfassung
2015
Titel
Cytotoxicity of natural products and derivatives toward MCF-7 cell monolayers and cancer stem-like mammospheres.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
22

Data source: Europe PubMed Central

Abstract
UNLABELLED: Although cancer stem-like cells (CSCs) are rare, they can enter a non-proliferative or dormant state and resist therapy. Furthermore, quiescent CSCs are responsible for metastases that can appear after curative surgical treatment of a primary tumor. Because of drug resistance of CSCs, the development of novel therapies is urgently required that specifically target CSCs. PURPOSE: The aim of the present study was to investigate the potential of a panel of natural products and derivatives to inhibit CSC-enriched mammospheres of MCF-7 breast cancer cells. METHODS: CD44(high)/CD24(low) cells were identified by flow cytometry and maintained as mammospheres. As a control, we used two clinically established anticancer drugs (5-fluorouracil and docetaxel). A panel of natural products, shikonin, two cajanin stilbene acid (CSA) derivatives and artesunate were tested by resazurin assay on CSC-enriched mammospheres and MCF-7 monolayer cells. Besides, cellular shikonin uptake experiments were performed by flow cytometry. RESULTS: We found two CSA derivatives (Nos. 6 and 19) to be active cancer stem-like MCF-7 mammospheres. Especially, CSA derivative No. 19 clearly showed collateral sensitivity in mammospheres compared to monolayer cells. CONCLUSION: Phytochemicals which provoke collateral sensitivity in cancer-stem like cells are worth more detailed investigations in the future, since there is a great potential for improved chemotherapy to eradicate tumors and prolong cancer patients' survival times.
Date of acceptance
2015
Autoren
  • Ean-Jeong Seo
  • Benjamin Wiench
  • Rebecca Hamm
  • Malte Paulsen
  • Yuangang Zu
  • Yujie Fu
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/25925965
DOI
10.1016/j.phymed.2015.01.012
eISSN
1618-095X
Ausgabe der Veröffentlichung
4
Zeitschrift
Phytomedicine
Schlüsselwörter
  • Artesunate
  • Cajanin stilbene acid (CSA)
  • Cancer stem-like cells
  • Drug resistance
  • Mammospheres
  • Shikonin
  • Antineoplastic Agents
  • Artemisinins
  • Artesunate
  • Humans
  • MCF-7 Cells
  • Naphthoquinones
  • Neoplastic Stem Cells
  • Phytochemicals
  • Salicylates
  • Spheroids, Cellular
  • Stilbenes
Sprache
eng
Country
Germany
Paginierung
438 - 443
PII
S0944-7113(15)00035-5
Datum der Veröffentlichung
2015
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2015
Titel
Cytotoxicity of natural products and derivatives toward MCF-7 cell monolayers and cancer stem-like mammospheres.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
22

Data source: PubMed

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