Kaempferol Is an Anti-Inflammatory Compound with Activity towards NF-xB Pathway Proteins

Publication type:
Journal article
Metadata:
Autoren
  • Onat Kadioglu
  • Janine Nass
  • Mohamed EM Saeed
  • Barbara Schuler
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000354267200021&DestLinkType=FullRecord&DestApp=WOS_CPL
eISSN
1791-7530
Externe Identifier
  • Clarivate Analytics Document Solution ID: CH8DU
  • PubMed Identifier: 25964540
ISSN
0250-7005
Ausgabe der Veröffentlichung
5
Zeitschrift
ANTICANCER RESEARCH
Schlüsselwörter
  • inflammation
  • leukemia
  • molecular docking
  • NF-xB pathway
Paginierung
2645 - 2650
Datum der Veröffentlichung
2015
Status
Published
Titel
Kaempferol Is an Anti-Inflammatory Compound with Activity towards NF-<i>x</i>B Pathway Proteins
Sub types
  • Article
Ausgabe der Zeitschrift
35

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
<h4>Background</h4>The nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway is critical in inflammation, proliferation and carcinogenesis. There exist three main players in this pathway. The inhibitor of NF-κB (IκB), IκB kinase (IκK)- NF-κB essential modulator (NEMO) complex and NF-κB. The IkK-NEMO complex activates NF-κB via phosphorylation of Iκβ and, eventually, leads to its proteasomal degradation. This leads to nuclear translocation of NF-κB and activation of target genes, such as cyclooxygenases and interleukins. The identification of anti-inflammatory compounds might be an effective strategy to target inflammatory disorders and cancer.<h4>Materials and methods</h4>In the present investigation, kaempferol was investigated in terms of its effect on NF-κB activity with a SEAP-driven reporter cell line, NF-κB DNA binding with electromobility shift assay (EMSA) and translocation of NF-κB-p65 from cytosol to the nucleus with western blot in Jurkat cells.<h4>Results</h4>Kaempferol revealed anti-inflammatory activity, as shown in vitro and in silico. Molecular docking studies of kaempferol revealed comparable binding energies and similar docking poses on target proteins such as MG-132, a known NF-κB inhibitor.<h4>Conclusion</h4>We conclude that kaempferol possesses anti-inflammatory activity.
Addresses
  • Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Mainz, Germany.
Autoren
  • Onat Kadioglu
  • Janine Nass
  • Mohamed EM Saeed
  • Barbara Schuler
  • Thomas Efferth
eISSN
1791-7530
Externe Identifier
  • PubMed Identifier: 25964540
Open access
false
ISSN
0250-7005
Ausgabe der Veröffentlichung
5
Zeitschrift
Anticancer research
Schlüsselwörter
  • Jurkat Cells
  • Humans
  • Leukemia
  • Inflammation
  • Kaempferols
  • NF-kappa B
  • Signal Transduction
  • Apoptosis
  • Cell Proliferation
  • Phosphorylation
  • I-kappa B Kinase
  • Molecular Docking Simulation
  • Carcinogenesis
Sprache
eng
Medium
Print
Paginierung
2645 - 2650
Datum der Veröffentlichung
2015
Status
Published
Datum der Datenerfassung
2015
Titel
Kaempferol Is an Anti-Inflammatory Compound with Activity towards NF-κB Pathway Proteins.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
35

Data source: Europe PubMed Central

Abstract
BACKGROUND: The nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway is critical in inflammation, proliferation and carcinogenesis. There exist three main players in this pathway. The inhibitor of NF-κB (IκB), IκB kinase (IκK)- NF-κB essential modulator (NEMO) complex and NF-κB. The IkK-NEMO complex activates NF-κB via phosphorylation of Iκβ and, eventually, leads to its proteasomal degradation. This leads to nuclear translocation of NF-κB and activation of target genes, such as cyclooxygenases and interleukins. The identification of anti-inflammatory compounds might be an effective strategy to target inflammatory disorders and cancer. MATERIALS AND METHODS: In the present investigation, kaempferol was investigated in terms of its effect on NF-κB activity with a SEAP-driven reporter cell line, NF-κB DNA binding with electromobility shift assay (EMSA) and translocation of NF-κB-p65 from cytosol to the nucleus with western blot in Jurkat cells. RESULTS: Kaempferol revealed anti-inflammatory activity, as shown in vitro and in silico. Molecular docking studies of kaempferol revealed comparable binding energies and similar docking poses on target proteins such as MG-132, a known NF-κB inhibitor. CONCLUSION: We conclude that kaempferol possesses anti-inflammatory activity.
Autoren
  • Onat Kadioglu
  • Janine Nass
  • Mohamed EM Saeed
  • Barbara Schuler
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/25964540
eISSN
1791-7530
Ausgabe der Veröffentlichung
5
Zeitschrift
Anticancer Res
Schlüsselwörter
  • NF-κB pathway
  • inflammation
  • leukemia
  • molecular docking
  • Apoptosis
  • Carcinogenesis
  • Cell Proliferation
  • Humans
  • I-kappa B Kinase
  • Inflammation
  • Jurkat Cells
  • Kaempferols
  • Leukemia
  • Molecular Docking Simulation
  • NF-kappa B
  • Phosphorylation
  • Signal Transduction
Sprache
eng
Country
Greece
Paginierung
2645 - 2650
PII
35/5/2645
Datum der Veröffentlichung
2015
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2015
Titel
Kaempferol Is an Anti-Inflammatory Compound with Activity towards NF-κB Pathway Proteins.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
35

Data source: PubMed

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