Cell cycle arrest and induction of apoptosis by cajanin stilbene acid from Cajanus cajun in breast cancer cells

Publication type:
Journal article
Metadata:
Autoren
  • Yujie Fu
  • Onat Kadioglu
  • Benjamin Wiench
  • Zuofu Wei
  • Chang Gao
  • Meng Luo
  • Chengbo Gu
  • Yuangang Zu
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000353556300006&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.phymed.2015.02.005
eISSN
1618-095X
Externe Identifier
  • Clarivate Analytics Document Solution ID: CG8KL
  • PubMed Identifier: 25925968
ISSN
0944-7113
Ausgabe der Veröffentlichung
4
Zeitschrift
PHYTOMEDICINE
Schlüsselwörter
  • Apoptosis
  • Breast cancer
  • Cell cycle
  • Microarray
  • Pharmacogenomics
  • Fabaceae
Paginierung
462 - 468
Datum der Veröffentlichung
2015
Status
Published
Titel
Cell cycle arrest and induction of apoptosis by cajanin stilbene acid from <i>Cajanus</i> <i>cajun</i> in breast cancer cells
Sub types
  • Article
Ausgabe der Zeitschrift
22

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Yujie Fu
  • Onat Kadioglu
  • Benjamin Wiench
  • Zuofu Wei
  • Chang Gao
  • Meng Luo
  • Chengbo Gu
  • Yuangang Zu
  • Thomas Efferth
DOI
10.1016/j.phymed.2015.02.005
ISSN
0944-7113
Ausgabe der Veröffentlichung
4
Zeitschrift
Phytomedicine
Sprache
en
Paginierung
462 - 468
Datum der Veröffentlichung
2015
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.phymed.2015.02.005
Datum der Datenerfassung
2023
Titel
Cell cycle arrest and induction of apoptosis by cajanin stilbene acid from Cajanus cajan in breast cancer cells
Ausgabe der Zeitschrift
22

Data source: Crossref

Abstract
<h4>Background</h4>The low abundant cajanin stilbene acid (CSA) from Pigeon Pea (Cajanus cajan) has been shown to kill estrogen receptor α positive cancer cells in vitro and in vivo. Downstream effects such as cell cycle and apoptosis-related mechanisms have not been analyzed yet.<h4>Material and methods</h4>We analyzed the activity of CSA by means of flow cytometry (cell cycle distribution, mitochondrial membrane potential, MMP), confocal laser scanning microscopy (MMP), DNA fragmentation assay (apoptosis), Western blotting (Bax and Bcl-2 expression, caspase-3 activation) as well as mRNA microarray hybridization and Ingenuity pathway analysis.<h4>Results</h4>CSA induced G2/M arrest and apoptosis in a concentration-dependent manner from 8.88 to 14.79 µM. The MMP broke down, Bax was upregulated, Bcl-2 downregulated and caspase-3 activated. Microarray profiling revealed that CSA affected BRCA-related DNA damage response and cell cycle-regulated chromosomal replication pathways.<h4>Conclusion</h4>CSA inhibited breast cancer cells by DNA damage and cell cycle-related signaling pathways leading to cell cycle arrest and apoptosis.
Addresses
  • Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin, China.
Autoren
  • Yujie Fu
  • Onat Kadioglu
  • Benjamin Wiench
  • Zuofu Wei
  • Chang Gao
  • Meng Luo
  • Chengbo Gu
  • Yuangang Zu
  • Thomas Efferth
  • Thomas Efferth
DOI
10.1016/j.phymed.2015.02.005
eISSN
1618-095X
Externe Identifier
  • PubMed Identifier: 25925968
Open access
false
ISSN
0944-7113
Ausgabe der Veröffentlichung
4
Zeitschrift
Phytomedicine : international journal of phytotherapy and phytopharmacology
Schlüsselwörter
  • Humans
  • Cajanus
  • DNA Damage
  • Salicylates
  • Stilbenes
  • Proto-Oncogene Proteins c-bcl-2
  • Signal Transduction
  • Apoptosis
  • Gene Expression Regulation, Neoplastic
  • bcl-2-Associated X Protein
  • Caspase 3
  • Membrane Potential, Mitochondrial
  • Cell Cycle Checkpoints
  • MCF-7 Cells
Sprache
eng
Medium
Print-Electronic
Online publication date
2015
Paginierung
462 - 468
Datum der Veröffentlichung
2015
Status
Published
Datum der Datenerfassung
2015
Titel
Cell cycle arrest and induction of apoptosis by cajanin stilbene acid from Cajanus cajan in breast cancer cells.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
22

Data source: Europe PubMed Central

Abstract
BACKGROUND: The low abundant cajanin stilbene acid (CSA) from Pigeon Pea (Cajanus cajan) has been shown to kill estrogen receptor α positive cancer cells in vitro and in vivo. Downstream effects such as cell cycle and apoptosis-related mechanisms have not been analyzed yet. MATERIAL AND METHODS: We analyzed the activity of CSA by means of flow cytometry (cell cycle distribution, mitochondrial membrane potential, MMP), confocal laser scanning microscopy (MMP), DNA fragmentation assay (apoptosis), Western blotting (Bax and Bcl-2 expression, caspase-3 activation) as well as mRNA microarray hybridization and Ingenuity pathway analysis. RESULTS: CSA induced G2/M arrest and apoptosis in a concentration-dependent manner from 8.88 to 14.79 µM. The MMP broke down, Bax was upregulated, Bcl-2 downregulated and caspase-3 activated. Microarray profiling revealed that CSA affected BRCA-related DNA damage response and cell cycle-regulated chromosomal replication pathways. CONCLUSION: CSA inhibited breast cancer cells by DNA damage and cell cycle-related signaling pathways leading to cell cycle arrest and apoptosis.
Date of acceptance
2015
Autoren
  • Yujie Fu
  • Onat Kadioglu
  • Benjamin Wiench
  • Zuofu Wei
  • Chang Gao
  • Meng Luo
  • Chengbo Gu
  • Yuangang Zu
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/25925968
DOI
10.1016/j.phymed.2015.02.005
eISSN
1618-095X
Ausgabe der Veröffentlichung
4
Zeitschrift
Phytomedicine
Schlüsselwörter
  • Apoptosis
  • Breast cancer
  • Cell cycle
  • Fabaceae
  • Microarray
  • Pharmacogenomics
  • Apoptosis
  • Cajanus
  • Caspase 3
  • Cell Cycle Checkpoints
  • DNA Damage
  • Gene Expression Regulation, Neoplastic
  • Humans
  • MCF-7 Cells
  • Membrane Potential, Mitochondrial
  • Proto-Oncogene Proteins c-bcl-2
  • Salicylates
  • Signal Transduction
  • Stilbenes
  • bcl-2-Associated X Protein
Sprache
eng
Country
Germany
Paginierung
462 - 468
PII
S0944-7113(15)00052-5
Datum der Veröffentlichung
2015
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2015
Titel
Cell cycle arrest and induction of apoptosis by cajanin stilbene acid from Cajanus cajan in breast cancer cells.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
22

Data source: PubMed

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