Bacteria-Derived Compatible Solutes Ectoine and 5α-Hydroxyectoine Act as Intestinal Barrier Stabilizers to Ameliorate Experimental Inflammatory Bowel Disease

Autoren

Heba Abdel-Aziz
Walaa Wadie
Olaf Scherner
Thomas Efferth
Mohamed T Khayyal

DOI

10.1021/acs.jnatprod.5b00115

eISSN

1520-6025

ISSN

0163-3864

Ausgabe der Veröffentlichung

6

Zeitschrift

Journal of Natural Products

Sprache

en

Online publication date

2015

Paginierung

1309 - 1315

Datum der Veröffentlichung

2015

Status

Published

Herausgeber

American Chemical Society (ACS)

Herausgeber URL

http://dx.doi.org/10.1021/acs.jnatprod.5b00115

Datum der Datenerfassung

2023

Titel

Bacteria-Derived Compatible Solutes Ectoine and 5α-Hydroxyectoine Act as Intestinal Barrier Stabilizers to Ameliorate Experimental Inflammatory Bowel Disease

Ausgabe der Zeitschrift

78

Data source: Crossref

Abstract

Earlier studies showed that the compatible solute ectoine (1) given prophylactically before induction of colitis by 2,4,6-trinitrobenzenesulfonic acid (TNBS) in rats prevented histological changes induced in the colon and the associated rise in inflammatory mediators. This study was therefore conducted to investigate whether ectoine (1) and its 5α-hydroxy derivative (2) would also be effective in treating an already established condition. Two days after inducing colitis in rats by instilling TNBS/alcohol in the colon, animals were treated orally once daily for 1 week with either 1 or 2 (50, 100, 300 mg/kg). Twenty-four hours after the last drug administration rats were sacrificed. Ulcerative lesions and colon mass indices were reduced by 1 and 2 in a bell-shaped manner. Best results were obtained with 100 mg/kg ectoine (1) and 50 mg/kg 5α-hydroxyectoine (2). The solutes normalized the rise in myeloperoxidase, TNFα, and IL-1β induced by TNBS but did not affect levels of reduced glutathione or ICAM-1, while reducing the level of fecal calprotectin, an established marker for inflammatory bowel disease. The findings indicate that the naturally occurring compatible solutes ectoine (1) and 5α-hydroxyectoine (2) possess an optimum concentration that affords maximal intestinal barrier stabilization and could therefore prove useful for better management of human inflammatory bowel disease.

Addresses

†Scientific Department, Steigerwald Arzneimittelwerk GmbH, Havelstraße 5, Darmstadt, Germany.

Autoren

Heba Abdel-Aziz
Walaa Wadie
Olaf Scherner
Thomas Efferth
Thomas Efferth
Mohamed T Khayyal

DOI

10.1021/acs.jnatprod.5b00115

eISSN

1520-6025

Externe Identifier

PubMed Identifier: 26020571

Funding acknowledgements

Bitop AG:

Open access

false

ISSN

0163-3864

Ausgabe der Veröffentlichung

6

Zeitschrift

Journal of natural products

Schlüsselwörter

Intestines
Colon
Animals
Humans
Rats
Rats, Wistar
Bacteria
Colitis
Inflammatory Bowel Diseases
Trinitrobenzenesulfonic Acid
Peroxidase
Amino Acids, Diamino
Tumor Necrosis Factor-alpha
Glutathione
Intercellular Adhesion Molecule-1
Solutions
Molecular Structure
Egypt
Male
Interleukin-1beta

Sprache

eng

Medium

Print-Electronic

Online publication date

2015

Paginierung

1309 - 1315

Datum der Veröffentlichung

2015

Status

Published

Datum der Datenerfassung

2015

Titel

Bacteria-Derived Compatible Solutes Ectoine and 5α-Hydroxyectoine Act as Intestinal Barrier Stabilizers to Ameliorate Experimental Inflammatory Bowel Disease.

Sub types

Journal Article

Ausgabe der Zeitschrift

78

Data source: Europe PubMed Central

Abstract

Earlier studies showed that the compatible solute ectoine (1) given prophylactically before induction of colitis by 2,4,6-trinitrobenzenesulfonic acid (TNBS) in rats prevented histological changes induced in the colon and the associated rise in inflammatory mediators. This study was therefore conducted to investigate whether ectoine (1) and its 5α-hydroxy derivative (2) would also be effective in treating an already established condition. Two days after inducing colitis in rats by instilling TNBS/alcohol in the colon, animals were treated orally once daily for 1 week with either 1 or 2 (50, 100, 300 mg/kg). Twenty-four hours after the last drug administration rats were sacrificed. Ulcerative lesions and colon mass indices were reduced by 1 and 2 in a bell-shaped manner. Best results were obtained with 100 mg/kg ectoine (1) and 50 mg/kg 5α-hydroxyectoine (2). The solutes normalized the rise in myeloperoxidase, TNFα, and IL-1β induced by TNBS but did not affect levels of reduced glutathione or ICAM-1, while reducing the level of fecal calprotectin, an established marker for inflammatory bowel disease. The findings indicate that the naturally occurring compatible solutes ectoine (1) and 5α-hydroxyectoine (2) possess an optimum concentration that affords maximal intestinal barrier stabilization and could therefore prove useful for better management of human inflammatory bowel disease.

Autoren

Heba Abdel-Aziz
Walaa Wadie
Olaf Scherner
Thomas Efferth
Mohamed T Khayyal

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/26020571

DOI

10.1021/acs.jnatprod.5b00115

eISSN

1520-6025

Ausgabe der Veröffentlichung

6

Zeitschrift

J Nat Prod

Schlüsselwörter

Amino Acids, Diamino
Animals
Bacteria
Colitis
Colon
Egypt
Glutathione
Humans
Inflammatory Bowel Diseases
Intercellular Adhesion Molecule-1
Interleukin-1beta
Intestines
Male
Molecular Structure
Peroxidase
Rats
Rats, Wistar
Solutions
Trinitrobenzenesulfonic Acid
Tumor Necrosis Factor-alpha

Sprache

eng

Country

United States

Paginierung

1309 - 1315

Datum der Veröffentlichung

2015

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2015

Titel

Bacteria-Derived Compatible Solutes Ectoine and 5α-Hydroxyectoine Act as Intestinal Barrier Stabilizers to Ameliorate Experimental Inflammatory Bowel Disease.

Sub types

Journal Article

Ausgabe der Zeitschrift

78

Data source: PubMed

Bacteria-Derived Compatible Solutes Ectoine and 5α-Hydroxyectoine Act as Intestinal Barrier Stabilizers to Ameliorate Experimental Inflammatory Bowel Disease

Tools