Cytotoxicity of anthraquinones from the roots of Pentas schimperi towards multi-factorial drug-resistant cancer cells
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Victor Kuete
- Arno R Nanfack Donfack
- Armelle T Mbaveng
- Maen Zeino
- Pierre Tane
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000357462700008&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1007/s10637-015-0268-9
- eISSN
- 1573-0646
- Externe Identifier
- Clarivate Analytics Document Solution ID: CM1TH
- PubMed Identifier: 26115800
- ISSN
- 0167-6997
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- INVESTIGATIONAL NEW DRUGS
- Schlüsselwörter
- Anthraquinones
- Apoptosis
- Damnacanthal
- Cytotoxicity
- Pentas schimperi
- Rubiaceae
- Paginierung
- 861 - 869
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Titel
- Cytotoxicity of anthraquinones from the roots of <i>Pentas schimperi</i> towards multi-factorial drug-resistant cancer cells
- Sub types
- Article
- Ausgabe der Zeitschrift
- 33
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Victor Kuete
- Arno R Nanfack Donfack
- Armelle T Mbaveng
- Maen Zeino
- Pierre Tane
- Thomas Efferth
- DOI
- 10.1007/s10637-015-0268-9
- eISSN
- 1573-0646
- ISSN
- 0167-6997
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Investigational New Drugs
- Sprache
- en
- Online publication date
- 2015
- Paginierung
- 861 - 869
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1007/s10637-015-0268-9
- Datum der Datenerfassung
- 2023
- Titel
- Cytotoxicity of anthraquinones from the roots of Pentas schimperi towards multi-factorial drug-resistant cancer cells
- Ausgabe der Zeitschrift
- 33
Data source: Crossref
- Abstract
- <h4>Introduction</h4>Multidrug resistance in cancer represents a major problem in chemotherapy. The present study was designed to assess the cytotoxicity of anthraquinones from Pentas schimperi, namely damnacanthal (1), damnacanthol (2), 3-hydroxy-2-hydroxymethyl anthraquinone (3) and schimperiquinone B (4) against nine drug-sensitive and multidrug resistant (MDR) cancer cell lines.<h4>Methods</h4>The resazurin reduction assay was used to evaluate the cytotoxicity of the above compounds, whilst caspase-Glo assay was used to detect the activation of caspases enzymes by compounds 1 and 2. Cell cycle, mitochondrial membrane potential (MMP) and levels of reactive oxygen species were all analyzed via flow cytometry.<h4>Results</h4>Anthraquinones 1 and 2 displayed cytotoxic effects with IC50 values below 81 μM on all the nine tested cancer cell lines whilst 3 and 4 displayed selective activities. The recorded IC50 values for compounds 1 and 2 ranged from 3.12 μM and 12.18 μM (towards leukemia CCRF-CEM cells) and from 30.32 μM and 80.11 μM (towards gliobastoma U87MG.ΔEGFR cells) respectively, and from 0.20 μM (against CCRF-CEM cells) to 195.12 μM (against CEM/ADR5000 cells) for doxorubicin. Compounds 1 and 2 induced apoptosis in CCRF-CEM leukemia cells, mediated by the disruption of the MMP and increase in ROS production.<h4>Conclusions</h4>Anthraquinones from Pentas schimperi and mostly 1 and 2 are potential cytotoxic natural products that deserve more investigations to develop novel antineoplastic drugs against multifactorial drug resistant cancers.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 5, 55128, Mainz, Germany.
- Autoren
- Victor Kuete
- Arno R Nanfack Donfack
- Armelle T Mbaveng
- Maen Zeino
- Pierre Tane
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1007/s10637-015-0268-9
- eISSN
- 1573-0646
- Externe Identifier
- PubMed Identifier: 26115800
- Open access
- false
- ISSN
- 0167-6997
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Investigational new drugs
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Rubiaceae
- Plant Roots
- Reactive Oxygen Species
- Anthraquinones
- Caspases
- Plant Extracts
- Antineoplastic Agents, Phytogenic
- Drug Resistance, Multiple
- Cell Cycle
- Apoptosis
- Drug Resistance, Neoplasm
- Membrane Potential, Mitochondrial
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2015
- Paginierung
- 861 - 869
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Datum der Datenerfassung
- 2015
- Titel
- Cytotoxicity of anthraquinones from the roots of Pentas schimperi towards multi-factorial drug-resistant cancer cells.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 33
Data source: Europe PubMed Central
- Abstract
- INTRODUCTION: Multidrug resistance in cancer represents a major problem in chemotherapy. The present study was designed to assess the cytotoxicity of anthraquinones from Pentas schimperi, namely damnacanthal (1), damnacanthol (2), 3-hydroxy-2-hydroxymethyl anthraquinone (3) and schimperiquinone B (4) against nine drug-sensitive and multidrug resistant (MDR) cancer cell lines. METHODS: The resazurin reduction assay was used to evaluate the cytotoxicity of the above compounds, whilst caspase-Glo assay was used to detect the activation of caspases enzymes by compounds 1 and 2. Cell cycle, mitochondrial membrane potential (MMP) and levels of reactive oxygen species were all analyzed via flow cytometry. RESULTS: Anthraquinones 1 and 2 displayed cytotoxic effects with IC50 values below 81 μM on all the nine tested cancer cell lines whilst 3 and 4 displayed selective activities. The recorded IC50 values for compounds 1 and 2 ranged from 3.12 μM and 12.18 μM (towards leukemia CCRF-CEM cells) and from 30.32 μM and 80.11 μM (towards gliobastoma U87MG.ΔEGFR cells) respectively, and from 0.20 μM (against CCRF-CEM cells) to 195.12 μM (against CEM/ADR5000 cells) for doxorubicin. Compounds 1 and 2 induced apoptosis in CCRF-CEM leukemia cells, mediated by the disruption of the MMP and increase in ROS production. CONCLUSIONS: Anthraquinones from Pentas schimperi and mostly 1 and 2 are potential cytotoxic natural products that deserve more investigations to develop novel antineoplastic drugs against multifactorial drug resistant cancers.
- Date of acceptance
- 2015
- Autoren
- Victor Kuete
- Arno R Nanfack Donfack
- Armelle T Mbaveng
- Maen Zeino
- Pierre Tane
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/26115800
- DOI
- 10.1007/s10637-015-0268-9
- eISSN
- 1573-0646
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Invest New Drugs
- Schlüsselwörter
- Anthraquinones
- Antineoplastic Agents, Phytogenic
- Apoptosis
- Caspases
- Cell Cycle
- Cell Line, Tumor
- Drug Resistance, Multiple
- Drug Resistance, Neoplasm
- Humans
- Membrane Potential, Mitochondrial
- Plant Extracts
- Plant Roots
- Reactive Oxygen Species
- Rubiaceae
- Sprache
- eng
- Country
- United States
- Paginierung
- 861 - 869
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2016
- Titel
- Cytotoxicity of anthraquinones from the roots of Pentas schimperi towards multi-factorial drug-resistant cancer cells.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 33
Data source: PubMed
- Beziehungen:
- Property of