Cytotoxicity of 35 medicinal plants from Sudan towards sensitive and multidrug-resistant cancer cells
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Mohamed EM Saeed
- Haider Abdelgadir
- Yoshikazu Sugimoto
- Hassan E Khand
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000365050900064&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.jep.2015.07.005
- eISSN
- 1872-7573
- Externe Identifier
- Clarivate Analytics Document Solution ID: CW5QG
- PubMed Identifier: 26165828
- ISSN
- 0378-8741
- Zeitschrift
- JOURNAL OF ETHNOPHARMACOLOGY
- Schlüsselwörter
- Cytotoxicity
- Chemotherapy
- Multidrug resistance
- Sudanese medicinal plants
- Phytotherapy
- Microarray analysis
- Paginierung
- 644 - 658
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Titel
- Cytotoxicity of 35 medicinal plants from Sudan towards sensitive and multidrug-resistant cancer cells
- Sub types
- Article
- Ausgabe der Zeitschrift
- 174
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Mohamed EM Saeed
- Haider Abdelgadir
- Yoshikazu Sugimoto
- Hassan E Khalid
- Thomas Efferth
- DOI
- 10.1016/j.jep.2015.07.005
- ISSN
- 0378-8741
- Zeitschrift
- Journal of Ethnopharmacology
- Sprache
- en
- Paginierung
- 644 - 658
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.jep.2015.07.005
- Datum der Datenerfassung
- 2019
- Titel
- Cytotoxicity of 35 medicinal plants from Sudan towards sensitive and multidrug-resistant cancer cells
- Ausgabe der Zeitschrift
- 174
Data source: Crossref
- Abstract
- <h4>Background</h4>Cancer is a complex disease with multiple genetic and epigenetic alterations. Since decades, the hallmark of cancer therapy is chemotherapy. Cytotoxic drugs erase rapidly dividing cells without sufficient differentiation between normal and cancerous cells resulting in severe side effects in normal tissues. Recently, strategies for cancer treatment focused on targeting specific proteins involved in tumor growth and progression. The present study was designed to investigate the cytotoxicity of 65 crude extracts from 35 Sudanese medicinal plants towards various cancer cell lines expressing molecular mechanisms of resistance towards classical chemotherapeutics (two ATP-binding cassette transporters, ABCB1 (P-glycoprotein) and ABCB5, tumor suppressor p53, epidermal growth factors receptor (EGFR). And the aim was to identify plant extracts and isolated compounds thereof with activity towards otherwise drug-resistant tumor cells.<h4>Methods</h4>Cold maceration was performed to obtain crude extracts from the plants. The resazurin assay was used to determine cytotoxicity of the plant extracts. Microarray-based mRNA expression profiling, COMPARE, and hierarchical cluster analyses were applied to identify, which genes correlate with sensitivity or resistance to ambrosin, the main constituent of the most active extract Ambrosia maritima.<h4>Results</h4>The results of the resazurin assay on different tumors showed that Lawsonia inermis, Trigonella foenum-graecum and Ambrosia maritma were the most active crude extracts. Ambrosin was selected as one active principle of A. maritima for microarray-based expression profiling. Genes from various functional groups (transcriptional regulators, signal transduction, membrane transporters, cytoskeleton organization, chaperones, immune system development and DNA repair) were significantly correlated with response of tumor cell lines to ambrosin.<h4>Conclusion</h4>The results revealed cytotoxicity and pharmacogenomics studies of Sudanese medicinal plants provide an attractive strategy for the development of novel cancer therapeutics with activity towards cell lines that resistance to established anticancer agents.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Mainz, Germany.
- Autoren
- Mohamed EM Saeed
- Haider Abdelgadir
- Yoshikazu Sugimoto
- Hassan E Khalid
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1016/j.jep.2015.07.005
- eISSN
- 1872-7573
- Externe Identifier
- PubMed Identifier: 26165828
- Open access
- false
- ISSN
- 0378-8741
- Zeitschrift
- Journal of ethnopharmacology
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Plants, Medicinal
- Sesquiterpenes
- Sesquiterpenes, Guaiane
- Oxazines
- Xanthenes
- Antineoplastic Agents, Phytogenic
- Indicators and Reagents
- Computational Biology
- Pharmacogenetics
- Drug Resistance, Multiple
- Cell Survival
- Drug Resistance, Neoplasm
- Sudan
- Tumor Suppressor Protein p53
- ATP Binding Cassette Transporter, Subfamily B
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2015
- Paginierung
- 644 - 658
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Datum der Datenerfassung
- 2015
- Titel
- Cytotoxicity of 35 medicinal plants from Sudan towards sensitive and multidrug-resistant cancer cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 174
Data source: Europe PubMed Central
- Abstract
- BACKGROUND: Cancer is a complex disease with multiple genetic and epigenetic alterations. Since decades, the hallmark of cancer therapy is chemotherapy. Cytotoxic drugs erase rapidly dividing cells without sufficient differentiation between normal and cancerous cells resulting in severe side effects in normal tissues. Recently, strategies for cancer treatment focused on targeting specific proteins involved in tumor growth and progression. The present study was designed to investigate the cytotoxicity of 65 crude extracts from 35 Sudanese medicinal plants towards various cancer cell lines expressing molecular mechanisms of resistance towards classical chemotherapeutics (two ATP-binding cassette transporters, ABCB1 (P-glycoprotein) and ABCB5, tumor suppressor p53, epidermal growth factors receptor (EGFR). And the aim was to identify plant extracts and isolated compounds thereof with activity towards otherwise drug-resistant tumor cells. METHODS: Cold maceration was performed to obtain crude extracts from the plants. The resazurin assay was used to determine cytotoxicity of the plant extracts. Microarray-based mRNA expression profiling, COMPARE, and hierarchical cluster analyses were applied to identify, which genes correlate with sensitivity or resistance to ambrosin, the main constituent of the most active extract Ambrosia maritima. RESULTS: The results of the resazurin assay on different tumors showed that Lawsonia inermis, Trigonella foenum-graecum and Ambrosia maritma were the most active crude extracts. Ambrosin was selected as one active principle of A. maritima for microarray-based expression profiling. Genes from various functional groups (transcriptional regulators, signal transduction, membrane transporters, cytoskeleton organization, chaperones, immune system development and DNA repair) were significantly correlated with response of tumor cell lines to ambrosin. CONCLUSION: The results revealed cytotoxicity and pharmacogenomics studies of Sudanese medicinal plants provide an attractive strategy for the development of novel cancer therapeutics with activity towards cell lines that resistance to established anticancer agents.
- Date of acceptance
- 2015
- Autoren
- Mohamed EM Saeed
- Haider Abdelgadir
- Yoshikazu Sugimoto
- Hassan E Khalid
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/26165828
- DOI
- 10.1016/j.jep.2015.07.005
- eISSN
- 1872-7573
- Zeitschrift
- J Ethnopharmacol
- Schlüsselwörter
- Chemotherapy
- Cytotoxicity
- Microarray analysis
- Multidrug resistance
- Phytotherapy
- Sudanese medicinal plants
- ATP Binding Cassette Transporter, Subfamily B
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Antineoplastic Agents, Phytogenic
- Cell Line, Tumor
- Cell Survival
- Computational Biology
- Drug Resistance, Multiple
- Drug Resistance, Neoplasm
- Humans
- Indicators and Reagents
- Oxazines
- Pharmacogenetics
- Plants, Medicinal
- Sesquiterpenes
- Sesquiterpenes, Guaiane
- Sudan
- Tumor Suppressor Protein p53
- Xanthenes
- Sprache
- eng
- Country
- Ireland
- Paginierung
- 644 - 658
- PII
- S0378-8741(15)30022-2
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2016
- Titel
- Cytotoxicity of 35 medicinal plants from Sudan towards sensitive and multidrug-resistant cancer cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 174
Data source: PubMed
- Beziehungen:
- Property of