Cytotoxicity of two naturally occurring flavonoids (dorsmanin F and poinsettifolin B) towards multi-factorial drug-resistant cancer cells
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Victor Kuete
- Armelle T Mbaveng
- Maen Zeino
- Bathelemy Ngameni
- Gilbert Deccaux WF Kapche
- Simeon E Kouam
- Bonaventure T Ngadjui
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000359278500007&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.phymed.2015.04.007
- eISSN
- 1618-095X
- Externe Identifier
- Clarivate Analytics Document Solution ID: CO6PL
- PubMed Identifier: 26141760
- ISSN
- 0944-7113
- Ausgabe der Veröffentlichung
- 7-8
- Zeitschrift
- PHYTOMEDICINE
- Schlüsselwörter
- Apoptosis
- Cytotoxicity
- Dorsmanin F
- Flavonoids
- Multi-drug resistance
- Poinsettifolin B
- Paginierung
- 737 - 743
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Titel
- Cytotoxicity of two naturally occurring flavonoids (dorsmanin F and poinsettifolin B) towards multi-factorial drug-resistant cancer cells
- Sub types
- Article
- Ausgabe der Zeitschrift
- 22
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Victor Kuete
- Armelle T Mbaveng
- Maen Zeino
- Bathelemy Ngameni
- Gilbert Deccaux WF Kapche
- Simeon F Kouam
- Bonaventure T Ngadjui
- Thomas Efferth
- DOI
- 10.1016/j.phymed.2015.04.007
- ISSN
- 0944-7113
- Ausgabe der Veröffentlichung
- 7-8
- Zeitschrift
- Phytomedicine
- Sprache
- en
- Paginierung
- 737 - 743
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.phymed.2015.04.007
- Datum der Datenerfassung
- 2023
- Titel
- Cytotoxicity of two naturally occurring flavonoids (dorsmanin F and poinsettifolin B) towards multi-factorial drug-resistant cancer cells
- Ausgabe der Zeitschrift
- 22
Data source: Crossref
- Abstract
- <h4>Introduction</h4>The expression of diverse resistance mechanisms in cancer cells is one of the major barriers to successful cancer chemotherapy.<h4>Methods</h4>In the present study, we assessed the cytotoxicity of two naturally occurring flavonoids dorsmanin F (1, a flavanone) and poinsettifolin B (2, a chalcone) against 9 drug-sensitive and multidrug-resistant (MDR) cancer cell lines. The resazurin reduction assay was used to evaluate the cytotoxicity of these compounds, whilst caspase-Glo assay was used to detect caspase activation. Cell cycle, mitochondrial membrane potential (MMP) and levels of reactive oxygen species (ROS) were all analysed via flow cytometry.<h4>Results</h4>Compounds 1 and 2 displayed cytotoxic effects with IC50 values below 34 µM in all the 9 tested cancer cell lines. The IC50 values for flavanone 1 and chalcone 2 ranged from 5.34 µM and 1.94 µM (towards leukaemia CCRF-CEM cells) to 33.30 µM and 28.92 µM (towards MDA-MB-231-BCRP cells), respectively, and from 0.20 µM (against CCRF-CEM cells) to 195.12 µM (against CEM/ADR5000 cells) for doxorubicin. The compounds induced apoptosis in CCRF-CEM leukaemia cells, mediated by MMP disruption and increased ROS production.<h4>Conclusions</h4>Dorsmain F and poinsettifolin B are potential cytotoxic natural products that deserve more investigations to develop novel antineoplastic drugs against multifactorial drug-resistant cancers.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany ; Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.
- Autoren
- Victor Kuete
- Armelle T Mbaveng
- Maen Zeino
- Bathelemy Ngameni
- Gilbert Deccaux WF Kapche
- Simeon F Kouam
- Bonaventure T Ngadjui
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1016/j.phymed.2015.04.007
- eISSN
- 1618-095X
- Externe Identifier
- PubMed Identifier: 26141760
- Funding acknowledgements
- DAAD:
- Open access
- false
- ISSN
- 0944-7113
- Ausgabe der Veröffentlichung
- 7-8
- Zeitschrift
- Phytomedicine : international journal of phytotherapy and phytopharmacology
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Reactive Oxygen Species
- Chalcones
- Flavonoids
- Caspases
- Antineoplastic Agents, Phytogenic
- Inhibitory Concentration 50
- Drug Resistance, Multiple
- Cell Cycle
- Apoptosis
- Molecular Structure
- Drug Resistance, Neoplasm
- Membrane Potential, Mitochondrial
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2015
- Paginierung
- 737 - 743
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Datum der Datenerfassung
- 2015
- Titel
- Cytotoxicity of two naturally occurring flavonoids (dorsmanin F and poinsettifolin B) towards multi-factorial drug-resistant cancer cells.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 22
Data source: Europe PubMed Central
- Abstract
- INTRODUCTION: The expression of diverse resistance mechanisms in cancer cells is one of the major barriers to successful cancer chemotherapy. METHODS: In the present study, we assessed the cytotoxicity of two naturally occurring flavonoids dorsmanin F (1, a flavanone) and poinsettifolin B (2, a chalcone) against 9 drug-sensitive and multidrug-resistant (MDR) cancer cell lines. The resazurin reduction assay was used to evaluate the cytotoxicity of these compounds, whilst caspase-Glo assay was used to detect caspase activation. Cell cycle, mitochondrial membrane potential (MMP) and levels of reactive oxygen species (ROS) were all analysed via flow cytometry. RESULTS: Compounds 1 and 2 displayed cytotoxic effects with IC50 values below 34 µM in all the 9 tested cancer cell lines. The IC50 values for flavanone 1 and chalcone 2 ranged from 5.34 µM and 1.94 µM (towards leukaemia CCRF-CEM cells) to 33.30 µM and 28.92 µM (towards MDA-MB-231-BCRP cells), respectively, and from 0.20 µM (against CCRF-CEM cells) to 195.12 µM (against CEM/ADR5000 cells) for doxorubicin. The compounds induced apoptosis in CCRF-CEM leukaemia cells, mediated by MMP disruption and increased ROS production. CONCLUSIONS: Dorsmain F and poinsettifolin B are potential cytotoxic natural products that deserve more investigations to develop novel antineoplastic drugs against multifactorial drug-resistant cancers.
- Date of acceptance
- 2015
- Autoren
- Victor Kuete
- Armelle T Mbaveng
- Maen Zeino
- Bathelemy Ngameni
- Gilbert Deccaux WF Kapche
- Simeon F Kouam
- Bonaventure T Ngadjui
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/26141760
- DOI
- 10.1016/j.phymed.2015.04.007
- eISSN
- 1618-095X
- Ausgabe der Veröffentlichung
- 7-8
- Zeitschrift
- Phytomedicine
- Schlüsselwörter
- Apoptosis
- Cytotoxicity
- Dorsmanin F
- Flavonoids
- Multi-drug resistance
- Poinsettifolin B
- Antineoplastic Agents, Phytogenic
- Apoptosis
- Caspases
- Cell Cycle
- Cell Line, Tumor
- Chalcones
- Drug Resistance, Multiple
- Drug Resistance, Neoplasm
- Flavonoids
- Humans
- Inhibitory Concentration 50
- Membrane Potential, Mitochondrial
- Molecular Structure
- Reactive Oxygen Species
- Sprache
- eng
- Country
- Germany
- Paginierung
- 737 - 743
- PII
- S0944-7113(15)00114-2
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2015
- Titel
- Cytotoxicity of two naturally occurring flavonoids (dorsmanin F and poinsettifolin B) towards multi-factorial drug-resistant cancer cells.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 22
Data source: PubMed
- Beziehungen:
- Property of