Human ABCB1 confers cells resistance to cytotoxic guanidine alkaloids from Pterogyne nitens

Publication type:
Journal article
Metadata:
Autoren
  • Kazuhiro Satake
  • Megumi Tsukamoto
  • Yuji Mitani
  • Luis Octavio Regasini
  • Vanderlan da Silva Bolzani
  • Thomas Efferth
  • Hiroshi Nakagawa
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000356538400003&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.3233/BME-151282
eISSN
1878-3619
Externe Identifier
  • Clarivate Analytics Document Solution ID: CK9CP
  • PubMed Identifier: 26407111
ISSN
0959-2989
Ausgabe der Veröffentlichung
3
Zeitschrift
BIO-MEDICAL MATERIALS AND ENGINEERING
Schlüsselwörter
  • ABCB1
  • P-glycoprotein
  • ATP-binding cassette (ABC) transporter
  • guanidine alkaloids
Paginierung
249 - 256
Datum der Veröffentlichung
2015
Status
Published
Titel
Human ABCB1 confers cells resistance to cytotoxic guanidine alkaloids from <i>Pterogyne nitens</i>
Sub types
  • Article
Ausgabe der Zeitschrift
25

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Kazuhiro Satake
  • Megumi Tsukamoto
  • Yuji Mitani
  • Luis Octavio Regasini
  • Vanderlan da Silva Bolzani
  • Thomas Efferth
  • Hiroshi Nakagawa
DOI
10.3233/bme-151282
eISSN
1878-3619
ISSN
0959-2989
Ausgabe der Veröffentlichung
3
Zeitschrift
Bio-Medical Materials and Engineering
Paginierung
249 - 256
Datum der Veröffentlichung
2015
Status
Published
Herausgeber
IOS Press
Herausgeber URL
http://dx.doi.org/10.3233/bme-151282
Datum der Datenerfassung
2019
Titel
Human ABCB1 confers cells resistance to cytotoxic guanidine alkaloids from Pterogyne nitens
Ausgabe der Zeitschrift
25

Data source: Crossref

Abstract
Multidrug resistance (MDR) caused by human ABCB1 (P-glycoprotein/MDR1) is one of the major obstacles in chemotherapy. To understand the mechanism of MDR by ABCB1 and circumvent the MDR, in the present study, we established human ABCB1-expressing cells (Flp-In-293/ABCB1 cells) and examined the cytotoxic effects of four guanidine alkaloids from Pterogyne nitens (galegine, nitensidine A, pterogynidine and pterogynine) using Flp-In-293/Mock and Flp-In-293/ABCB1 cells. The activity of ABCB1 in Flp-In-293/ABCB1 cells were confirmed by typical substrates for ABCB1 (taxol and vinblastine) in MTT assay. Flp-In-293/ABCB1 cells were also resistant to the four guanidine alkaloids as well as taxol and vinblastine compared to Flp-In-293/Mock cells although the four guanidine alkaloids exhibited cytotoxicity against the two Flp-In-293 cells. Furthermore, the four guanidine alkaloids were also found to stimulate the ATPase activity of ABCB1 in ATPase assays. These results suggest that ABCB1 can confer the resistance to the cytotoxic guanidine alkaloids by transporting them.
Addresses
  • Department of Applied Biological Chemistry, Graduate School of Bioscience and Biotechnology, Chubu University, Kasugai, Japan.
Autoren
  • Kazuhiro Satake
  • Megumi Tsukamoto
  • Yuji Mitani
  • Luis Octavio Regasini
  • Vanderlan da Silva Bolzani
  • Thomas Efferth
  • Thomas Efferth
  • Hiroshi Nakagawa
DOI
10.3233/bme-151282
eISSN
1878-3619
Externe Identifier
  • PubMed Identifier: 26407111
Open access
false
ISSN
0959-2989
Ausgabe der Veröffentlichung
3
Zeitschrift
Bio-medical materials and engineering
Schlüsselwörter
  • Humans
  • Caesalpinia
  • Guanidines
  • Alkaloids
  • Multidrug Resistance-Associated Proteins
  • Cytotoxins
  • Drug Resistance, Multiple
  • Cell Survival
  • Dose-Response Relationship, Drug
  • HEK293 Cells
  • ATP Binding Cassette Transporter, Subfamily B
Sprache
eng
Medium
Print
Paginierung
249 - 256
Datum der Veröffentlichung
2015
Status
Published
Datum der Datenerfassung
2015
Titel
Human ABCB1 confers cells resistance to cytotoxic guanidine alkaloids from Pterogyne nitens.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
25

Data source: Europe PubMed Central

Abstract
Multidrug resistance (MDR) caused by human ABCB1 (P-glycoprotein/MDR1) is one of the major obstacles in chemotherapy. To understand the mechanism of MDR by ABCB1 and circumvent the MDR, in the present study, we established human ABCB1-expressing cells (Flp-In-293/ABCB1 cells) and examined the cytotoxic effects of four guanidine alkaloids from Pterogyne nitens (galegine, nitensidine A, pterogynidine and pterogynine) using Flp-In-293/Mock and Flp-In-293/ABCB1 cells. The activity of ABCB1 in Flp-In-293/ABCB1 cells were confirmed by typical substrates for ABCB1 (taxol and vinblastine) in MTT assay. Flp-In-293/ABCB1 cells were also resistant to the four guanidine alkaloids as well as taxol and vinblastine compared to Flp-In-293/Mock cells although the four guanidine alkaloids exhibited cytotoxicity against the two Flp-In-293 cells. Furthermore, the four guanidine alkaloids were also found to stimulate the ATPase activity of ABCB1 in ATPase assays. These results suggest that ABCB1 can confer the resistance to the cytotoxic guanidine alkaloids by transporting them.
Autoren
  • Kazuhiro Satake
  • Megumi Tsukamoto
  • Yuji Mitani
  • Luis Octavio Regasini
  • Vanderlan da Silva Bolzani
  • Thomas Efferth
  • Hiroshi Nakagawa
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/26407111
DOI
10.3233/BME-151282
eISSN
1878-3619
Ausgabe der Veröffentlichung
3
Zeitschrift
Biomed Mater Eng
Schlüsselwörter
  • ABCB1
  • ATP-binding cassette (ABC) transporter
  • P-glycoprotein
  • guanidine alkaloids
  • ATP Binding Cassette Transporter, Subfamily B
  • Alkaloids
  • Caesalpinia
  • Cell Survival
  • Cytotoxins
  • Dose-Response Relationship, Drug
  • Drug Resistance, Multiple
  • Guanidines
  • HEK293 Cells
  • Humans
  • Multidrug Resistance-Associated Proteins
Sprache
eng
Country
Netherlands
Paginierung
249 - 256
PII
BME1282
Datum der Veröffentlichung
2015
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2016
Titel
Human ABCB1 confers cells resistance to cytotoxic guanidine alkaloids from Pterogyne nitens.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
25

Data source: PubMed

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