Cytotoxicity of compounds from Xylopia aethiopica towards multi-factorial drug-resistant cancer cells
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Victor Kuete
- Louis P Sandjo
- Armelle T Mbaveng
- Maen Zeino
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000366983400006&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.phymed.2015.10.008
- Externe Identifier
- Clarivate Analytics Document Solution ID: CZ3DA
- PubMed Identifier: 26655407
- ISSN
- 0944-7113
- Ausgabe der Veröffentlichung
- 14
- Zeitschrift
- PHYTOMEDICINE
- Schlüsselwörter
- 3,4,5-trihydroxy-6 '',6 ''-dimethylpyrano[2,3-g]flavone
- Annonaceae
- Cancer
- Cytotoxicity
- Isotetrandrine
- Xylopia aethiopica
- Paginierung
- 1247 - 1254
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Titel
- Cytotoxicity of compounds from <i>Xylopia aethiopica</i> towards multi-factorial drug-resistant cancer cells
- Sub types
- Article
- Ausgabe der Zeitschrift
- 22
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Victor Kuete
- Louis P Sandjo
- Armelle T Mbaveng
- Maen Zeino
- Thomas Efferth
- DOI
- 10.1016/j.phymed.2015.10.008
- ISSN
- 0944-7113
- Ausgabe der Veröffentlichung
- 14
- Zeitschrift
- Phytomedicine
- Sprache
- en
- Paginierung
- 1247 - 1254
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.phymed.2015.10.008
- Datum der Datenerfassung
- 2019
- Titel
- Cytotoxicity of compounds from Xylopia aethiopica towards multi-factorial drug-resistant cancer cells
- Ausgabe der Zeitschrift
- 22
Data source: Crossref
- Abstract
- <h4>Introduction</h4>Multidrug resistance (MDR) in cancer represent a major hurdle in chemotherapy. Previously, the methanol extract of the medicinal spice Xylopia aethiopica displayed considerable cytotoxicity against multidrug resistant (MDR) cancer cell lines.<h4>Methods</h4>The present study was designed to assess the cytotoxicity of compounds, 16α-hydroxy-ent-kauran-19-oic acid (2), 3,4',5-trihydroxy-6″,6″-dimethylpyrano[2,3-g]flavone (3), isotetrandrine (5) and trans-tiliroside (6) derived from the methanol crude extract of Xylopia aethiopica against 9 drug-sensitive and -resistant cancer cell lines. The resazurin reduction assay was used to evaluate the cytotoxicity of these compounds, whilst caspase-Glo assay was used to detect caspase activation. Cell cycle, mitochondrial membrane potential (MMP) and levels of reactive oxygen species (ROS) were all analyzed via flow cytometry.<h4>Results</h4>Flavonoid 3 and alkaloid 5 also displayed IC50 values ranging from 2.61 µM (towards leukemia CCRF-CEM cells) to 18.60 µM (towards gliobastoma multiforme U87MG.ΔEGFR cells) and from 1.45 µM (towards HepG2 cells) to 7.28 µM (towards MDA-MB-231-pcDNA cells), respectively. IC50 values ranged from 0.20 µM (against CCRF-CEM cells) to 195.12 µM (against CEM/ADR5000 cells) for doxorubicin. Compound 3 induced apoptosis in leukemia CCRF-CEM cells mediated by the disruption of the MMP, whilst 5 induced apoptosis mediated by ROS production.<h4>Conclusions</h4>Compounds 2 and 5 represent potential cytotoxic phytochemicals that deserve more investigations to develop novel antineoplastic drugs against multifactorial drug-resistant cancers.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany; Department of Biochemistry, Faculty of Science, University of Dschang, Cameroon.
- Autoren
- Victor Kuete
- Louis P Sandjo
- Armelle T Mbaveng
- Maen Zeino
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1016/j.phymed.2015.10.008
- eISSN
- 1618-095X
- Externe Identifier
- PubMed Identifier: 26655407
- Open access
- false
- ISSN
- 0944-7113
- Ausgabe der Veröffentlichung
- 14
- Zeitschrift
- Phytomedicine : international journal of phytotherapy and phytopharmacology
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Reactive Oxygen Species
- Alkaloids
- Flavonoids
- Flavones
- Doxorubicin
- Caspases
- Plant Extracts
- Antineoplastic Agents
- Antineoplastic Agents, Phytogenic
- Drug Resistance, Multiple
- Cell Cycle
- Apoptosis
- Molecular Structure
- Drug Resistance, Neoplasm
- Membrane Potential, Mitochondrial
- Xylopia
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2015
- Paginierung
- 1247 - 1254
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Datum der Datenerfassung
- 2015
- Titel
- Cytotoxicity of compounds from Xylopia aethiopica towards multi-factorial drug-resistant cancer cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 22
Data source: Europe PubMed Central
- Abstract
- INTRODUCTION: Multidrug resistance (MDR) in cancer represent a major hurdle in chemotherapy. Previously, the methanol extract of the medicinal spice Xylopia aethiopica displayed considerable cytotoxicity against multidrug resistant (MDR) cancer cell lines. METHODS: The present study was designed to assess the cytotoxicity of compounds, 16α-hydroxy-ent-kauran-19-oic acid (2), 3,4',5-trihydroxy-6″,6″-dimethylpyrano[2,3-g]flavone (3), isotetrandrine (5) and trans-tiliroside (6) derived from the methanol crude extract of Xylopia aethiopica against 9 drug-sensitive and -resistant cancer cell lines. The resazurin reduction assay was used to evaluate the cytotoxicity of these compounds, whilst caspase-Glo assay was used to detect caspase activation. Cell cycle, mitochondrial membrane potential (MMP) and levels of reactive oxygen species (ROS) were all analyzed via flow cytometry. RESULTS: Flavonoid 3 and alkaloid 5 also displayed IC50 values ranging from 2.61 µM (towards leukemia CCRF-CEM cells) to 18.60 µM (towards gliobastoma multiforme U87MG.ΔEGFR cells) and from 1.45 µM (towards HepG2 cells) to 7.28 µM (towards MDA-MB-231-pcDNA cells), respectively. IC50 values ranged from 0.20 µM (against CCRF-CEM cells) to 195.12 µM (against CEM/ADR5000 cells) for doxorubicin. Compound 3 induced apoptosis in leukemia CCRF-CEM cells mediated by the disruption of the MMP, whilst 5 induced apoptosis mediated by ROS production. CONCLUSIONS: Compounds 2 and 5 represent potential cytotoxic phytochemicals that deserve more investigations to develop novel antineoplastic drugs against multifactorial drug-resistant cancers.
- Date of acceptance
- 2015
- Autoren
- Victor Kuete
- Louis P Sandjo
- Armelle T Mbaveng
- Maen Zeino
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/26655407
- DOI
- 10.1016/j.phymed.2015.10.008
- eISSN
- 1618-095X
- Ausgabe der Veröffentlichung
- 14
- Zeitschrift
- Phytomedicine
- Schlüsselwörter
- 3,4′,5-trihydroxy-6″,6″-dimethylpyrano[2,3-g]flavone
- Annonaceae
- Cancer
- Cytotoxicity
- Isotetrandrine
- Xylopia aethiopica
- Alkaloids
- Antineoplastic Agents
- Antineoplastic Agents, Phytogenic
- Apoptosis
- Caspases
- Cell Cycle
- Cell Line, Tumor
- Doxorubicin
- Drug Resistance, Multiple
- Drug Resistance, Neoplasm
- Flavones
- Flavonoids
- Humans
- Membrane Potential, Mitochondrial
- Molecular Structure
- Plant Extracts
- Reactive Oxygen Species
- Xylopia
- Sprache
- eng
- Country
- Germany
- Paginierung
- 1247 - 1254
- PII
- S0944-7113(15)00320-7
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2016
- Titel
- Cytotoxicity of compounds from Xylopia aethiopica towards multi-factorial drug-resistant cancer cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 22
Data source: PubMed
- Beziehungen:
- Property of