Cytotoxicity of 91 Kenyan indigenous medicinal plants towards human CCRF-CEM leukemia cells

Publication type:
Journal article
Metadata:
Autoren
  • Leonidah K Omosa
  • Jacob O Midiwo
  • Veronica M Masila
  • Boniface M Gisacho
  • Renee Munayi
  • Francisca-Kamakama
  • Kitur Phylis Chemutai
  • Gihan Elhaboob
  • Mohamed EM Saeed
  • Sami Hamdoun
  • Victor Kuete
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000370884400021&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.jep.2015.12.028
eISSN
1872-7573
Externe Identifier
  • Clarivate Analytics Document Solution ID: DE8KH
  • PubMed Identifier: 26721219
ISSN
0378-8741
Zeitschrift
JOURNAL OF ETHNOPHARMACOLOGY
Schlüsselwörter
  • Albizia schimperiana
  • Solanum aculeastrum
  • Cancer
  • Kenyan flora
  • Leukemia
  • Traditional medicine
Paginierung
177 - 196
Datum der Veröffentlichung
2016
Status
Published
Titel
Cytotoxicity of 91 Kenyan indigenous medicinal plants towards human CCRF-CEM leukemia cells
Sub types
  • Article
Ausgabe der Zeitschrift
179

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Leonidah K Omosa
  • Jacob O Midiwo
  • Veronica M Masila
  • Boniface M Gisacho
  • Renee Munayi
  • Francisca-Kamakama
  • Kitur Phylis Chemutai
  • Gihan Elhaboob
  • Mohamed EM Saeed
  • Sami Hamdoun
  • Victor Kuete
  • Thomas Efferth
DOI
10.1016/j.jep.2015.12.028
ISSN
0378-8741
Zeitschrift
Journal of Ethnopharmacology
Sprache
en
Paginierung
177 - 196
Datum der Veröffentlichung
2016
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.jep.2015.12.028
Datum der Datenerfassung
2023
Titel
Cytotoxicity of 91 Kenyan indigenous medicinal plants towards human CCRF-CEM leukemia cells
Ausgabe der Zeitschrift
179

Data source: Crossref

Abstract
<h4>Ethnopharmacological relevance</h4>Plants from Kenyan flora are traditionally used against many ailments, including cancer and related diseases. Cancer is characterized as a condition with complex signs and symptoms. Recently there are recommendations that ethnopharmacological usages such as immune and skin disorders, inflammatory, infectious, parasitic and viral diseases should be taken into account when selecting plants that treat cancer.<h4>Aim</h4>The present study was aimed at investigating the cytotoxicity of a plethora of 145 plant parts from 91 medicinal plants, most of which are used in the management of cancer and related diseases by different communities in Kenya, against CCRF-CEM leukemia cell line.<h4>Materials and methods</h4>Extracts from different plant parts (leaves, stems, stem bark, roots, root barks, aerial parts and whole herb) were obtained by cold percolation using different solvent systems, such as (1:1v/v) dichloromethane (CH2Cl2) and n-hexane (1), methanol (MeOH) and CH2Cl2 (2); neat MeOH (3), 5% H2O in MeOH (4) and with ethanol (EtOH, 5); their cytotoxicities were determined using the resazurin reduction assay against CCRF-CEM cells.<h4>Results</h4>At a single concentration of 10μg/mL, 12 out of 145 extracts exhibited more than 50% cell inhibition. These include samples from the root bark of Erythrina sacleuxii (extracted with 50% n-hexane-CH2Cl2), the leaves of Albizia gummifera, and Strychnos usambarensis, the stem bark of Zanthoxylum gilletii, Bridelia micrantha, Croton sylvaticus, and Albizia schimperiana; the root bark of Erythrina burttii and E. sacleuxii (extracted with 50% CH2Cl2-MeOH), the stem bark of B. micrantha and Z. gilletii (extracted using 5% MeOH-H2O) and from the berries of Solanum aculeastrum (extracted with neat EtOH). The EtOH extract of the berries of S. aculeastrum and A. schimperiana stem bark extract displayed the highest cytotoxicity towards leukemia CCRF-CEM cells, with IC50 values of 1.36 and 2.97µg/mL, respectively. Other extracts having good activities included the extracts of the stem barks of Z. gilletii and B. micrantha and leaves of S. usambarensis with IC50 values of 9.04, 9.43 and 11.09µg/mL, respectively.<h4>Conclusions</h4>The results of this study provided information related to the possible use of some Kenyam medicinal plants, and mostly S. aculeastrum, A. schimperiana, C. sylvaticus, Z. gilletii, B. micrantha and S. usambarensis in the treatment of leukemia. The reported data helped to authenticate the claimed traditional use of these plants. However, most plants are used in combination as traditional herbal concoctions. Hence, the cytotoxicity of corresponding plant combinations should be tested in vitro to authenticate the traditional medical practitioners actual practices.
Addresses
  • Department of Chemistry, School of Physical Sciences, University of Nairobi, P. O. Box 30197-00100, Nairobi, Kenya; Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Staudinger Weg 5, 55128 Mainz, Germany. Electronic address: lkerubo@uonbi.ac.ke.
Autoren
  • Leonidah K Omosa
  • Jacob O Midiwo
  • Veronica M Masila
  • Boniface M Gisacho
  • Renee Munayi
  • Francisca-Kamakama
  • Kitur Phylis Chemutai
  • Gihan Elhaboob
  • Mohamed EM Saeed
  • Sami Hamdoun
  • Victor Kuete
  • Thomas Efferth
  • Thomas Efferth
DOI
10.1016/j.jep.2015.12.028
eISSN
1872-7573
Externe Identifier
  • PubMed Identifier: 26721219
Funding acknowledgements
  • Uppsala University, Sweden: (ISP)-KEN-02
Open access
false
ISSN
0378-8741
Zeitschrift
Journal of ethnopharmacology
Schlüsselwörter
  • Cell Line, Tumor
  • Humans
  • Plants, Medicinal
  • Leukemia
  • Growth Inhibitors
  • Plant Extracts
  • Antineoplastic Agents, Phytogenic
  • Cell Survival
  • Kenya
Sprache
eng
Medium
Print-Electronic
Online publication date
2015
Paginierung
177 - 196
Datum der Veröffentlichung
2016
Status
Published
Datum der Datenerfassung
2016
Titel
Cytotoxicity of 91 Kenyan indigenous medicinal plants towards human CCRF-CEM leukemia cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
179

Data source: Europe PubMed Central

Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Plants from Kenyan flora are traditionally used against many ailments, including cancer and related diseases. Cancer is characterized as a condition with complex signs and symptoms. Recently there are recommendations that ethnopharmacological usages such as immune and skin disorders, inflammatory, infectious, parasitic and viral diseases should be taken into account when selecting plants that treat cancer. AIM: The present study was aimed at investigating the cytotoxicity of a plethora of 145 plant parts from 91 medicinal plants, most of which are used in the management of cancer and related diseases by different communities in Kenya, against CCRF-CEM leukemia cell line. MATERIALS AND METHODS: Extracts from different plant parts (leaves, stems, stem bark, roots, root barks, aerial parts and whole herb) were obtained by cold percolation using different solvent systems, such as (1:1v/v) dichloromethane (CH2Cl2) and n-hexane (1), methanol (MeOH) and CH2Cl2 (2); neat MeOH (3), 5% H2O in MeOH (4) and with ethanol (EtOH, 5); their cytotoxicities were determined using the resazurin reduction assay against CCRF-CEM cells. RESULTS: At a single concentration of 10μg/mL, 12 out of 145 extracts exhibited more than 50% cell inhibition. These include samples from the root bark of Erythrina sacleuxii (extracted with 50% n-hexane-CH2Cl2), the leaves of Albizia gummifera, and Strychnos usambarensis, the stem bark of Zanthoxylum gilletii, Bridelia micrantha, Croton sylvaticus, and Albizia schimperiana; the root bark of Erythrina burttii and E. sacleuxii (extracted with 50% CH2Cl2-MeOH), the stem bark of B. micrantha and Z. gilletii (extracted using 5% MeOH-H2O) and from the berries of Solanum aculeastrum (extracted with neat EtOH). The EtOH extract of the berries of S. aculeastrum and A. schimperiana stem bark extract displayed the highest cytotoxicity towards leukemia CCRF-CEM cells, with IC50 values of 1.36 and 2.97µg/mL, respectively. Other extracts having good activities included the extracts of the stem barks of Z. gilletii and B. micrantha and leaves of S. usambarensis with IC50 values of 9.04, 9.43 and 11.09µg/mL, respectively. CONCLUSIONS: The results of this study provided information related to the possible use of some Kenyam medicinal plants, and mostly S. aculeastrum, A. schimperiana, C. sylvaticus, Z. gilletii, B. micrantha and S. usambarensis in the treatment of leukemia. The reported data helped to authenticate the claimed traditional use of these plants. However, most plants are used in combination as traditional herbal concoctions. Hence, the cytotoxicity of corresponding plant combinations should be tested in vitro to authenticate the traditional medical practitioners actual practices.
Date of acceptance
2015
Autoren
  • Leonidah K Omosa
  • Jacob O Midiwo
  • Veronica M Masila
  • Boniface M Gisacho
  • Renee Munayi
  • Francisca-Kamakama
  • Kitur Phylis Chemutai
  • Gihan Elhaboob
  • Mohamed EM Saeed
  • Sami Hamdoun
  • Victor Kuete
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/26721219
DOI
10.1016/j.jep.2015.12.028
eISSN
1872-7573
Zeitschrift
J Ethnopharmacol
Schlüsselwörter
  • Albizia schimperiana
  • Cancer
  • Kenyan flora
  • Leukemia
  • Solanum aculeastrum
  • Traditional medicine
  • Antineoplastic Agents, Phytogenic
  • Cell Line, Tumor
  • Cell Survival
  • Growth Inhibitors
  • Humans
  • Kenya
  • Leukemia
  • Plant Extracts
  • Plants, Medicinal
Sprache
eng
Country
Ireland
Paginierung
177 - 196
PII
S0378-8741(15)30279-8
Datum der Veröffentlichung
2016
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2016
Titel
Cytotoxicity of 91 Kenyan indigenous medicinal plants towards human CCRF-CEM leukemia cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
179

Data source: PubMed

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