Production of rosmarinic acid and salvianolic acid B from callus culture of Salvia miltiorrhiza with cytotoxicity towards acute lymphoblastic leukemia cells
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Ching-Fen Wu
- Anastasia Karioti
- Doris Rohr
- Anna Rita Bilia
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000369494300040&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.foodchem.2016.01.054
- eISSN
- 1873-7072
- Externe Identifier
- Clarivate Analytics Document Solution ID: DC8UD
- PubMed Identifier: 26868579
- ISSN
- 0308-8146
- Zeitschrift
- FOOD CHEMISTRY
- Schlüsselwörter
- Callus culture
- High performance liquid chromatography (HPLC)
- CCRF-CEM cells
- Secondary metabolites
- Paginierung
- 292 - 297
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Titel
- Production of rosmarinic acid and salvianolic acid B from callus culture of <i>Salvia miltiorrhiza</i> with cytotoxicity towards acute lymphoblastic leukemia cells
- Sub types
- Article
- Ausgabe der Zeitschrift
- 201
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Ching-Fen Wu
- Anastasia Karioti
- Doris Rohr
- Anna Rita Bilia
- Thomas Efferth
- DOI
- 10.1016/j.foodchem.2016.01.054
- ISSN
- 0308-8146
- Zeitschrift
- Food Chemistry
- Sprache
- en
- Paginierung
- 292 - 297
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.foodchem.2016.01.054
- Datum der Datenerfassung
- 2018
- Titel
- Production of rosmarinic acid and salvianolic acid B from callus culture of Salvia miltiorrhiza with cytotoxicity towards acute lymphoblastic leukemia cells
- Ausgabe der Zeitschrift
- 201
Data source: Crossref
- Abstract
- Salvia miltiorrhiza (SM) Bunge is one of the widely-used Chinese medicinal herbs. In this study, the chemical constituents and anticancer potential of SM stems and leaves were examined with those of respective callus cultures. The callus culture for stem and leaf explants was initiated in modified Murashige and Skoog (MS) medium. Active constituents of respective extracts were analyzed by high performance liquid chromatography coupled with DAD and MS (HPLC-DAD-MS). Rosmarinic acid (RA) and salvianolic acid B (Sal B) were determined to be the main phenolic compounds. Quantitative analyses revealed that callus stem extracts produced higher amount of RA and Sal B (stem RA: 1.27±0.38%; stem Sal B: 0.87±0.20%) than callus leaf did (leaf RA: 0.28±0.02%; leaf Sal B: 0.07±0.03%). Stem and leaf callus extracts exerted cytotoxic effects towards CCRF-CEM cells (stem: 13.1±0.90 μg/ml; leaf: 18.1±0.33 μg/ml). As expected, stem extract with higher amount of RA and Sal B showed lower IC50 value than leaf extract. These findings suggest the possibility to isolate bioactive constituents with anticancer properties from in vitro callus cultures of stems and leaves of SM.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Staudingerweg 5, 55128 Mainz, Germany.
- Autoren
- Ching-Fen Wu
- Anastasia Karioti
- Doris Rohr
- Anna Rita Bilia
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1016/j.foodchem.2016.01.054
- eISSN
- 1873-7072
- Externe Identifier
- PubMed Identifier: 26868579
- Funding acknowledgements
- National Science Council Taiwan:
- DAAD:
- Open access
- false
- ISSN
- 1873-7072
- Zeitschrift
- Food chemistry
- Schlüsselwörter
- Salvia miltiorrhiza
- Plant Leaves
- Cinnamates
- Benzofurans
- Plant Extracts
- Drugs, Chinese Herbal
- Chromatography, High Pressure Liquid
- Depsides
- Precursor Cell Lymphoblastic Leukemia-Lymphoma
- Rosmarinic Acid
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2016
- Paginierung
- 292 - 297
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Datum der Datenerfassung
- 2016
- Titel
- Production of rosmarinic acid and salvianolic acid B from callus culture of Salvia miltiorrhiza with cytotoxicity towards acute lymphoblastic leukemia cells.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 201
Data source: Europe PubMed Central
- Abstract
- Salvia miltiorrhiza (SM) Bunge is one of the widely-used Chinese medicinal herbs. In this study, the chemical constituents and anticancer potential of SM stems and leaves were examined with those of respective callus cultures. The callus culture for stem and leaf explants was initiated in modified Murashige and Skoog (MS) medium. Active constituents of respective extracts were analyzed by high performance liquid chromatography coupled with DAD and MS (HPLC-DAD-MS). Rosmarinic acid (RA) and salvianolic acid B (Sal B) were determined to be the main phenolic compounds. Quantitative analyses revealed that callus stem extracts produced higher amount of RA and Sal B (stem RA: 1.27±0.38%; stem Sal B: 0.87±0.20%) than callus leaf did (leaf RA: 0.28±0.02%; leaf Sal B: 0.07±0.03%). Stem and leaf callus extracts exerted cytotoxic effects towards CCRF-CEM cells (stem: 13.1±0.90 μg/ml; leaf: 18.1±0.33 μg/ml). As expected, stem extract with higher amount of RA and Sal B showed lower IC50 value than leaf extract. These findings suggest the possibility to isolate bioactive constituents with anticancer properties from in vitro callus cultures of stems and leaves of SM.
- Date of acceptance
- 2016
- Autoren
- Ching-Fen Wu
- Anastasia Karioti
- Doris Rohr
- Anna Rita Bilia
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/26868579
- DOI
- 10.1016/j.foodchem.2016.01.054
- eISSN
- 1873-7072
- Zeitschrift
- Food Chem
- Schlüsselwörter
- CCRF-CEM cells
- Callus culture
- High performance liquid chromatography (HPLC)
- Secondary metabolites
- Benzofurans
- Chromatography, High Pressure Liquid
- Cinnamates
- Depsides
- Drugs, Chinese Herbal
- Plant Extracts
- Plant Leaves
- Precursor Cell Lymphoblastic Leukemia-Lymphoma
- Salvia miltiorrhiza
- Rosmarinic Acid
- Sprache
- eng
- Country
- England
- Paginierung
- 292 - 297
- PII
- S0308-8146(16)30054-1
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2016
- Titel
- Production of rosmarinic acid and salvianolic acid B from callus culture of Salvia miltiorrhiza with cytotoxicity towards acute lymphoblastic leukemia cells.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 201
Data source: PubMed
- Beziehungen:
- Property of