In Vivo Cardiotoxicity Induced by Sodium Aescinate in Zebrafish Larvae
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Jinfeng Liang
- Wangdong Jin
- Hongwen Li
- Hongcui Liu
- Yanfeng Huang
- Xiaowen Shan
- Chunqi Li
- Letian Shan
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000373802200133&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3390/molecules21030190
- Externe Identifier
- Clarivate Analytics Document Solution ID: DI9DR
- PubMed Identifier: 26907249
- ISSN
- 1420-3049
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- MOLECULES
- Schlüsselwörter
- sodium aescinate
- zebrafish
- larvae
- cardiotoxicity
- MNLC
- LC10
- Artikelnummer
- ARTN 190
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Titel
- <i>In Vivo</i> Cardiotoxicity Induced by Sodium Aescinate in Zebrafish Larvae
- Sub types
- Article
- Ausgabe der Zeitschrift
- 21
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Jinfeng Liang
- Wangdong Jin
- Hongwen Li
- Hongcui Liu
- Yanfeng Huang
- Xiaowen Shan
- Chunqi Li
- Letian Shan
- Thomas Efferth
- DOI
- 10.3390/molecules21030190
- eISSN
- 1420-3049
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Molecules
- Sprache
- en
- Online publication date
- 2016
- Paginierung
- 190 - 190
- Status
- Published online
- Herausgeber
- MDPI AG
- Herausgeber URL
- http://dx.doi.org/10.3390/molecules21030190
- Datum der Datenerfassung
- 2019
- Titel
- In Vivo Cardiotoxicity Induced by Sodium Aescinate in Zebrafish Larvae
- Ausgabe der Zeitschrift
- 21
Data source: Crossref
- Abstract
- Sodium aescinate (SA) is a widely-applied triterpene saponin product derived from horse chestnut seeds, possessing vasoactive and organ-protective activities with oral or injection administration in the clinic. To date, no toxicity or adverse events in SA have been reported, by using routine models (in vivo or in vitro), which are insufficient to predict all aspects of its pharmacological and toxicological actions. In this study, taking advantage of transparent zebrafish larvae (Danio rerio), we evaluated cardiovascular toxicity of SA at doses of 1/10 MNLC, 1/3 MNLC, MNLC and LC10 by yolk sac microinjection. The qualitative and quantitative cardiotoxicity in zebrafish was assessed at 48 h post-SA treatment, using specific phenotypic endpoints: heart rate, heart rhythm, heart malformation, pericardial edema, circulation abnormalities, thrombosis and hemorrhage. The results showed that SA at 1/10 MNLC and above doses could induce obvious cardiac and pericardial malformations, whilst 1/3 MNLC and above doses could induce significant cardiac malfunctions (heart rate and circulation decrease/absence), as compared to untreated or vehicle-treated control groups. Such cardiotoxic manifestations occurred in more than 50% to 100% of all zebrafish treated with SA at MNLC and LC10. Our findings have uncovered the potential cardiotoxicity of SA for the first time, suggesting more attention to the risk of its clinical application. Such a time- and cost-saving zebrafish cardiotoxicity assay is very valid and reliable for rapid prediction of compound toxicity during drug research and development.
- Addresses
- Zhejiang Center for Drugs and Cosmetics Evaluation, Zhejiang Province Food and Drug Administration, Hangzhou 310012, China. liangwind@sohu.com.
- Autoren
- Jinfeng Liang
- Wangdong Jin
- Hongwen Li
- Hongcui Liu
- Yanfeng Huang
- Xiaowen Shan
- Chunqi Li
- Letian Shan
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.3390/molecules21030190
- eISSN
- 1420-3049
- Externe Identifier
- PubMed Identifier: 26907249
- PubMed Central ID: PMC6273315
- Funding acknowledgements
- Ningbo Natural Science Foundation: 2014A610277
- National Natural Science Foundation of China: 81302989
- Open access
- true
- ISSN
- 1420-3049
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Molecules (Basel, Switzerland)
- Schlüsselwörter
- Heart
- Yolk Sac
- Embryo, Nonmammalian
- Animals
- Zebrafish
- Heart Defects, Congenital
- Thrombosis
- Hemorrhage
- Triterpenes
- Saponins
- Microinjections
- Drug Evaluation, Preclinical
- Toxicity Tests, Chronic
- Larva
- Heart Rate
- Dose-Response Relationship, Drug
- Cardiotoxicity
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2016
- Open access status
- Open Access
- Paginierung
- 190
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2016
- Titel
- In Vivo Cardiotoxicity Induced by Sodium Aescinate in Zebrafish Larvae.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 21
Files
https://www.mdpi.com/1420-3049/21/3/190/pdf?version=1456229672 https://europepmc.org/articles/PMC6273315?pdf=render
Data source: Europe PubMed Central
- Abstract
- Sodium aescinate (SA) is a widely-applied triterpene saponin product derived from horse chestnut seeds, possessing vasoactive and organ-protective activities with oral or injection administration in the clinic. To date, no toxicity or adverse events in SA have been reported, by using routine models (in vivo or in vitro), which are insufficient to predict all aspects of its pharmacological and toxicological actions. In this study, taking advantage of transparent zebrafish larvae (Danio rerio), we evaluated cardiovascular toxicity of SA at doses of 1/10 MNLC, 1/3 MNLC, MNLC and LC10 by yolk sac microinjection. The qualitative and quantitative cardiotoxicity in zebrafish was assessed at 48 h post-SA treatment, using specific phenotypic endpoints: heart rate, heart rhythm, heart malformation, pericardial edema, circulation abnormalities, thrombosis and hemorrhage. The results showed that SA at 1/10 MNLC and above doses could induce obvious cardiac and pericardial malformations, whilst 1/3 MNLC and above doses could induce significant cardiac malfunctions (heart rate and circulation decrease/absence), as compared to untreated or vehicle-treated control groups. Such cardiotoxic manifestations occurred in more than 50% to 100% of all zebrafish treated with SA at MNLC and LC10. Our findings have uncovered the potential cardiotoxicity of SA for the first time, suggesting more attention to the risk of its clinical application. Such a time- and cost-saving zebrafish cardiotoxicity assay is very valid and reliable for rapid prediction of compound toxicity during drug research and development.
- Date of acceptance
- 2016
- Autoren
- Jinfeng Liang
- Wangdong Jin
- Hongwen Li
- Hongcui Liu
- Yanfeng Huang
- Xiaowen Shan
- Chunqi Li
- Letian Shan
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/26907249
- DOI
- 10.3390/molecules21030190
- eISSN
- 1420-3049
- Externe Identifier
- PubMed Central ID: PMC6273315
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Molecules
- Schlüsselwörter
- LC10
- MNLC
- cardiotoxicity
- larvae
- sodium aescinate
- zebrafish
- Animals
- Cardiotoxicity
- Dose-Response Relationship, Drug
- Drug Evaluation, Preclinical
- Embryo, Nonmammalian
- Heart
- Heart Defects, Congenital
- Heart Rate
- Hemorrhage
- Larva
- Microinjections
- Saponins
- Thrombosis
- Toxicity Tests, Chronic
- Triterpenes
- Yolk Sac
- Zebrafish
- Sprache
- eng
- Country
- Switzerland
- Paginierung
- 190
- PII
- molecules21030190
- Datum der Veröffentlichung
- 2016
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2016
- Titel
- In Vivo Cardiotoxicity Induced by Sodium Aescinate in Zebrafish Larvae.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 21
Data source: PubMed
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