Cytotoxicity of South-African medicinal plants towards sensitive and multidrug-resistant cancer cells
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Mohamed EM Saeed
- Marion Meyer
- Ahmed Hussein
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000377832000023&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.jep.2016.04.005
- eISSN
- 1872-7573
- Externe Identifier
- Clarivate Analytics Document Solution ID: DO5OI
- PubMed Identifier: 27058630
- ISSN
- 0378-8741
- Zeitschrift
- JOURNAL OF ETHNOPHARMACOLOGY
- Schlüsselwörter
- ABC transporters
- Chemotherapy
- Multidrug resistance
- Natural products
- Pharmacogenomics
- Phytotherapy
- Paginierung
- 209 - 223
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Titel
- Cytotoxicity of South-African medicinal plants towards sensitive and multidrug-resistant cancer cells
- Sub types
- Article
- Ausgabe der Zeitschrift
- 186
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Mohamed EM Saeed
- Marion Meyer
- Ahmed Hussein
- Thomas Efferth
- DOI
- 10.1016/j.jep.2016.04.005
- ISSN
- 0378-8741
- Zeitschrift
- Journal of Ethnopharmacology
- Sprache
- en
- Paginierung
- 209 - 223
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.jep.2016.04.005
- Datum der Datenerfassung
- 2019
- Titel
- Cytotoxicity of South-African medicinal plants towards sensitive and multidrug-resistant cancer cells
- Ausgabe der Zeitschrift
- 186
Data source: Crossref
- Abstract
- <h4>Ethnopharmacological relevance</h4>Traditional medicine plays a major role for primary health care worldwide. Cancer belongs to the leading disease burden in industrialized and developing countries. Successful cancer therapy is hampered by the development of resistance towards established anticancer drugs.<h4>Aim</h4>In the present study, we investigated the cytotoxicity of 29 extracts from 26 medicinal plants of South-Africa against leukemia cell lines, most of which are used traditionally to treat cancer and related symptoms.<h4>Material and methods</h4>We have investigated the plant extracts for their cytotoxic activity towards drug-sensitive parental CCRF-CEM leukemia cells and their multidrug-resistant P-glycoprotein-overexpressing subline, CEM/ADR5000 by means of the resazurin assay. A panel of 60 NCI tumor cell lines have been investigated for correlations between selected phytochemicals from medicinal plants and the expression of resistance-conferring genes (ABC-transporters, oncogenes, tumor suppressor genes).<h4>Results</h4>Seven extracts inhibited both cell lines (Acokanthera oppositifolia, Hypoestes aristata, Laurus nobilis, Leonotis leonurus, Plectranthus barbatus, Plectranthus ciliates, Salvia apiana). CEM/ADR5000 cells exhibited a low degree of cross-resistance (3.35-fold) towards the L. leonurus extract, while no cross-resistance was observed to other plant extracts, although CEM/ADR5000 cells were highly resistant to clinically established drugs. The log10IC50 values for two out of 14 selected phytochemicals from these plants (acovenoside A and ouabain) of 60 tumor cell lines were correlated to the expression of ABC-transporters (ABCB1, ABCB5, ABCC1, ABCG2), oncogenes (EGFR, RAS) and tumor suppressors (TP53). Sensitivity or resistance of the cell lines were not statistically associated with the expression of these genes, indicating that multidrug-resistant, refractory tumors expressing these genes may still respond to acovenoside A and ouabain.<h4>Conclusion</h4>The bioactivity of South African medicinal plants may represent a basis for the development of strategies to treat multidrug-resistant tumors either by phytotherapeutic approaches with whole plant preparations or by classical drug development with isolated compounds such as acovenoside A or ouabain.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Mainz, Germany.
- Autoren
- Mohamed EM Saeed
- Marion Meyer
- Ahmed Hussein
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1016/j.jep.2016.04.005
- eISSN
- 1872-7573
- Externe Identifier
- PubMed Identifier: 27058630
- Open access
- false
- ISSN
- 0378-8741
- Zeitschrift
- Journal of ethnopharmacology
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Plants, Medicinal
- Neoplasms
- Plant Extracts
- Antineoplastic Agents, Phytogenic
- Medicine, African Traditional
- Drug Resistance, Multiple
- Cell Survival
- Drug Resistance, Neoplasm
- South Africa
- Magnoliopsida
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2016
- Paginierung
- 209 - 223
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Datum der Datenerfassung
- 2016
- Titel
- Cytotoxicity of South-African medicinal plants towards sensitive and multidrug-resistant cancer cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 186
Data source: Europe PubMed Central
- Abstract
- ETHNOPHARMACOLOGICAL RELEVANCE: Traditional medicine plays a major role for primary health care worldwide. Cancer belongs to the leading disease burden in industrialized and developing countries. Successful cancer therapy is hampered by the development of resistance towards established anticancer drugs. AIM: In the present study, we investigated the cytotoxicity of 29 extracts from 26 medicinal plants of South-Africa against leukemia cell lines, most of which are used traditionally to treat cancer and related symptoms. MATERIAL AND METHODS: We have investigated the plant extracts for their cytotoxic activity towards drug-sensitive parental CCRF-CEM leukemia cells and their multidrug-resistant P-glycoprotein-overexpressing subline, CEM/ADR5000 by means of the resazurin assay. A panel of 60 NCI tumor cell lines have been investigated for correlations between selected phytochemicals from medicinal plants and the expression of resistance-conferring genes (ABC-transporters, oncogenes, tumor suppressor genes). RESULTS: Seven extracts inhibited both cell lines (Acokanthera oppositifolia, Hypoestes aristata, Laurus nobilis, Leonotis leonurus, Plectranthus barbatus, Plectranthus ciliates, Salvia apiana). CEM/ADR5000 cells exhibited a low degree of cross-resistance (3.35-fold) towards the L. leonurus extract, while no cross-resistance was observed to other plant extracts, although CEM/ADR5000 cells were highly resistant to clinically established drugs. The log10IC50 values for two out of 14 selected phytochemicals from these plants (acovenoside A and ouabain) of 60 tumor cell lines were correlated to the expression of ABC-transporters (ABCB1, ABCB5, ABCC1, ABCG2), oncogenes (EGFR, RAS) and tumor suppressors (TP53). Sensitivity or resistance of the cell lines were not statistically associated with the expression of these genes, indicating that multidrug-resistant, refractory tumors expressing these genes may still respond to acovenoside A and ouabain. CONCLUSION: The bioactivity of South African medicinal plants may represent a basis for the development of strategies to treat multidrug-resistant tumors either by phytotherapeutic approaches with whole plant preparations or by classical drug development with isolated compounds such as acovenoside A or ouabain.
- Date of acceptance
- 2016
- Autoren
- Mohamed EM Saeed
- Marion Meyer
- Ahmed Hussein
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/27058630
- DOI
- 10.1016/j.jep.2016.04.005
- eISSN
- 1872-7573
- Zeitschrift
- J Ethnopharmacol
- Schlüsselwörter
- ABC transporters
- Chemotherapy
- Multidrug resistance
- Natural products
- Pharmacogenomics
- Phytotherapy
- Antineoplastic Agents, Phytogenic
- Cell Line, Tumor
- Cell Survival
- Drug Resistance, Multiple
- Drug Resistance, Neoplasm
- Humans
- Magnoliopsida
- Medicine, African Traditional
- Neoplasms
- Plant Extracts
- Plants, Medicinal
- South Africa
- Sprache
- eng
- Country
- Ireland
- Paginierung
- 209 - 223
- PII
- S0378-8741(16)30195-7
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2017
- Titel
- Cytotoxicity of South-African medicinal plants towards sensitive and multidrug-resistant cancer cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 186
Data source: PubMed
- Beziehungen:
- Property of