Cytotoxicity of 15 Cameroonian medicinal plants against drug sensitive and multi-drug resistant cancer cells

Publication type:
Journal article
Metadata:
Autoren
  • Victor Kuete
  • Doriane E Djeussi
  • Armelle T Mbaveng
  • Maen Zeino
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000377832000021&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.jep.2016.04.001
Externe Identifier
  • Clarivate Analytics Document Solution ID: DO5OI
  • PubMed Identifier: 27063984
ISSN
0378-8741
Zeitschrift
JOURNAL OF ETHNOPHARMACOLOGY
Schlüsselwörter
  • Apoptosis
  • Cameroon
  • Cytotoxicity
  • Erythrina sigmoidea
  • Medicinal plants
Paginierung
196 - 204
Datum der Veröffentlichung
2016
Status
Published
Titel
Cytotoxicity of 15 Cameroonian medicinal plants against drug sensitive and multi-drug resistant cancer cells
Sub types
  • Article
Ausgabe der Zeitschrift
186

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Victor Kuete
  • Doriane E Djeussi
  • Armelle T Mbaveng
  • Maen Zeino
  • Thomas Efferth
DOI
10.1016/j.jep.2016.04.001
ISSN
0378-8741
Zeitschrift
Journal of Ethnopharmacology
Sprache
en
Paginierung
196 - 204
Datum der Veröffentlichung
2016
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.jep.2016.04.001
Datum der Datenerfassung
2023
Titel
Cytotoxicity of 15 Cameroonian medicinal plants against drug sensitive and multi-drug resistant cancer cells
Ausgabe der Zeitschrift
186

Data source: Crossref

Abstract
<h4>Ethnopharmacological relevance</h4>Cameroonian medicinal plants are traditionally used to treat many ailments, including cancer and related diseases. Cancer is characterized as a condition with complex signs and symptoms. It has been recommended that ethnopharmacological usages such as immune and skin disorders, inflammatory, infectious, parasitic and viral diseases should be taken into account when selecting plants for anticancer screenings, since these reflect disease states bearing relevance to cancer or cancer-like symptoms.<h4>Aim of the study</h4>The present study aims at investigating 20 methanol extracts from 15 Cameroonian medicinal plants on a panel of human cancer cell lines, including various drug-resistant phenotypes. Possible modes of action of the of the most active plant were analyzed.<h4>Materials and methods</h4>Methanol extracts from different plant parts (leaves, bark, roots, fruits or whole plant) were evaluated for their cytotoxicity using resazurin reduction assay on a panel of nine sensitive and multi-drug resistant (MDR) cancer cell lines. Cell cycle, apoptosis, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS) were measured by flow cytometry.<h4>Results</h4>Prescreening of extracts at 80µg/mL showed that 6 extracts out of 20 inhibited more than 50% proliferation of leukemia CCRF-CEM cells; these include extracts from Anthocleista schweinfurthii fruits (ASF; 48.28%), Morus mesozygia bark (MMB; 42.76%), Nauclea latifolia bark (NLB; 38.75%), Tridesmostemon omphalocarpoides bark (TOB; 38.53%), Nauclea latifolia leaves (NLL; 35.17%) and Erythrina sigmoidea bark (ESB; 33.77%). Subsequent investigations revealed IC50 values below or around 20µg/mL for extracts from MMB, NLB, NLL and ESB towards sensitive CCRF-CEM cells and its resistant P-glycoprotein over-expressing subline CEM/ADR5000. The best extract, ESB also displayed IC50 values below 20µg/mL colon carcinoma HCT116 (p53(+/+)) cells with an IC50 value of 19.63µg/mL and it resistant p53 knockout subline HCT116 (p53(-)(/-)) with an IC50 value of 16.22µg/mL.<h4>Conclusion</h4>Erythrina sigmoidea, Anthocleista schweinfurthii, Morus mesozygia, Nauclea latifolia, Tridesmostemon omphalocarpoides used in African traditional medicine are good cytotoxic plants that can be exploited to develop phytomedicine to fight cancers including MDR phenotypes.
Addresses
  • Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany; Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon. Electronic address: kuetevictor@yahoo.fr.
Autoren
  • Victor Kuete
  • Doriane E Djeussi
  • Armelle T Mbaveng
  • Maen Zeino
  • Thomas Efferth
  • Thomas Efferth
DOI
10.1016/j.jep.2016.04.001
eISSN
1872-7573
Externe Identifier
  • PubMed Identifier: 27063984
Open access
false
ISSN
0378-8741
Zeitschrift
Journal of ethnopharmacology
Schlüsselwörter
  • Cell Line, Tumor
  • Humans
  • Plants, Medicinal
  • Neoplasms
  • Reactive Oxygen Species
  • Plant Extracts
  • Antineoplastic Agents, Phytogenic
  • Medicine, African Traditional
  • Phytotherapy
  • Drug Resistance, Multiple
  • Cell Proliferation
  • Drug Resistance, Neoplasm
  • Cameroon
  • Membrane Potential, Mitochondrial
  • Magnoliopsida
Sprache
eng
Medium
Print-Electronic
Online publication date
2016
Paginierung
196 - 204
Datum der Veröffentlichung
2016
Status
Published
Datum der Datenerfassung
2016
Titel
Cytotoxicity of 15 Cameroonian medicinal plants against drug sensitive and multi-drug resistant cancer cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
186

Data source: Europe PubMed Central

Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Cameroonian medicinal plants are traditionally used to treat many ailments, including cancer and related diseases. Cancer is characterized as a condition with complex signs and symptoms. It has been recommended that ethnopharmacological usages such as immune and skin disorders, inflammatory, infectious, parasitic and viral diseases should be taken into account when selecting plants for anticancer screenings, since these reflect disease states bearing relevance to cancer or cancer-like symptoms. AIM OF THE STUDY: The present study aims at investigating 20 methanol extracts from 15 Cameroonian medicinal plants on a panel of human cancer cell lines, including various drug-resistant phenotypes. Possible modes of action of the of the most active plant were analyzed. MATERIALS AND METHODS: Methanol extracts from different plant parts (leaves, bark, roots, fruits or whole plant) were evaluated for their cytotoxicity using resazurin reduction assay on a panel of nine sensitive and multi-drug resistant (MDR) cancer cell lines. Cell cycle, apoptosis, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS) were measured by flow cytometry. RESULTS: Prescreening of extracts at 80µg/mL showed that 6 extracts out of 20 inhibited more than 50% proliferation of leukemia CCRF-CEM cells; these include extracts from Anthocleista schweinfurthii fruits (ASF; 48.28%), Morus mesozygia bark (MMB; 42.76%), Nauclea latifolia bark (NLB; 38.75%), Tridesmostemon omphalocarpoides bark (TOB; 38.53%), Nauclea latifolia leaves (NLL; 35.17%) and Erythrina sigmoidea bark (ESB; 33.77%). Subsequent investigations revealed IC50 values below or around 20µg/mL for extracts from MMB, NLB, NLL and ESB towards sensitive CCRF-CEM cells and its resistant P-glycoprotein over-expressing subline CEM/ADR5000. The best extract, ESB also displayed IC50 values below 20µg/mL colon carcinoma HCT116 (p53(+/+)) cells with an IC50 value of 19.63µg/mL and it resistant p53 knockout subline HCT116 (p53(-)(/-)) with an IC50 value of 16.22µg/mL. CONCLUSION: Erythrina sigmoidea, Anthocleista schweinfurthii, Morus mesozygia, Nauclea latifolia, Tridesmostemon omphalocarpoides used in African traditional medicine are good cytotoxic plants that can be exploited to develop phytomedicine to fight cancers including MDR phenotypes.
Date of acceptance
2016
Autoren
  • Victor Kuete
  • Doriane E Djeussi
  • Armelle T Mbaveng
  • Maen Zeino
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/27063984
DOI
10.1016/j.jep.2016.04.001
eISSN
1872-7573
Zeitschrift
J Ethnopharmacol
Schlüsselwörter
  • Apoptosis
  • Cameroon
  • Cytotoxicity
  • Erythrina sigmoidea
  • Medicinal plants
  • Antineoplastic Agents, Phytogenic
  • Cameroon
  • Cell Line, Tumor
  • Cell Proliferation
  • Drug Resistance, Multiple
  • Drug Resistance, Neoplasm
  • Humans
  • Magnoliopsida
  • Medicine, African Traditional
  • Membrane Potential, Mitochondrial
  • Neoplasms
  • Phytotherapy
  • Plant Extracts
  • Plants, Medicinal
  • Reactive Oxygen Species
Sprache
eng
Country
Ireland
Paginierung
196 - 204
PII
S0378-8741(16)30192-1
Datum der Veröffentlichung
2016
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2017
Titel
Cytotoxicity of 15 Cameroonian medicinal plants against drug sensitive and multi-drug resistant cancer cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
186

Data source: PubMed

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