Cytotoxicity of seven naturally occurring phenolic compounds towards multi-factorial drug-resistant cancer cells
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Victor Kuete
- Armelle T Mbaveng
- Eric CN Nono
- Christophe C Simo
- Maen Zeino
- Augustin E Nkengfack
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000377544000008&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.phymed.2016.04.007
- Externe Identifier
- Clarivate Analytics Document Solution ID: DO1NA
- PubMed Identifier: 27288921
- ISSN
- 0944-7113
- Ausgabe der Veröffentlichung
- 8
- Zeitschrift
- PHYTOMEDICINE
- Schlüsselwörter
- Alpinumisoflavone
- Apoptosis
- Cytotoxicity
- Multi-drug resistance
- Phenolic compounds
- Pycnanthulignene
- Paginierung
- 856 - 863
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Titel
- Cytotoxicity of seven naturally occurring phenolic compounds towards multi-factorial drug-resistant cancer cells
- Sub types
- Article
- Ausgabe der Zeitschrift
- 23
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Victor Kuete
- Armelle T Mbaveng
- Eric CN Nono
- Christophe C Simo
- Maen Zeino
- Augustin E Nkengfack
- Thomas Efferth
- DOI
- 10.1016/j.phymed.2016.04.007
- ISSN
- 0944-7113
- Ausgabe der Veröffentlichung
- 8
- Zeitschrift
- Phytomedicine
- Sprache
- en
- Paginierung
- 856 - 863
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.phymed.2016.04.007
- Datum der Datenerfassung
- 2023
- Titel
- Cytotoxicity of seven naturally occurring phenolic compounds towards multi-factorial drug-resistant cancer cells
- Ausgabe der Zeitschrift
- 23
Data source: Crossref
- Abstract
- <h4>Introduction</h4>In medical oncology, multi-drug resistance (MDR) of cancer cells continues to be a major impediment. We are in quest of novel anti-proliferative agents to overcome drug-resistant tumor cells.<h4>Methods</h4>In the present study, we investigated the cytotoxicity of 7 naturally occurring phenolic compounds including two isoflavonoids alpinumisoflavone (1) and laburnetin (2), one biflavonoid amentoflavone (3), three lignans pycnanthulignene A (4), pycnanthulignene B (5), and syringaresinol (7) and one xanthone, euxanthone (6) against 9 drug-sensitive and MDR cancer cell lines. The resazurin reduction assay was used to evaluate the cytotoxicity of these compounds, whilst caspase-Glo assay was used to detect caspase activation. Cell cycle, mitochondrial membrane potential (MMP) and levels of reactive oxygen species (ROS) were all analyzed via flow cytometry.<h4>Results</h4>The IC50 values for the investigational phenolics ranged from 5.91 µM (towards leukemia CEM/ADR5000 cells) to 65.65 µM (towards drug-resistant breast adenocarcinoma MDA-MB-231-BCRP cells) for 1, 27.63 µM (towards leukemia CCRF-CEM cells) to 107.57 µM (towards MDA-MB-231-pcDNA cells) for 2, from 5.84 µM (towards CEM/ADR5000 cells) to 65.32 µM (towards colon carcinoma HCT116 (p53(-/-)) cells) for 4 and 0.20 µM (towards CCRF-CEM cells) to 195.12 µM (towards leukemia CEM/ADR5000) for doxorubicin. Phenolics 3, 5, 6 and 7 displayed selectivity cytotoxic effects on cancer cells lines. Compounds 1 and 4 induced apoptosis in CCRF-CEM cells, mediated by loss of MMP and increase ROS production.<h4>Conclusions</h4>The studied phenolics and mostly isoflavonoid 1 and lignan 4 are potential cytotoxic natural products that deserve more investigations to develop novel antineoplastic drugs against multifactorial drug-resistant cancers.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Mainz 55128, Germany; Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.
- Autoren
- Victor Kuete
- Armelle T Mbaveng
- Eric CN Nono
- Christophe C Simo
- Maen Zeino
- Augustin E Nkengfack
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1016/j.phymed.2016.04.007
- eISSN
- 1618-095X
- Externe Identifier
- PubMed Identifier: 27288921
- Open access
- false
- ISSN
- 0944-7113
- Ausgabe der Veröffentlichung
- 8
- Zeitschrift
- Phytomedicine : international journal of phytotherapy and phytopharmacology
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Neoplasms
- Reactive Oxygen Species
- Phenols
- Oxazines
- Xanthenes
- Caspases
- Antineoplastic Agents, Phytogenic
- Drug Resistance, Multiple
- Enzyme Activation
- Drug Resistance, Neoplasm
- Membrane Potential, Mitochondrial
- Cell Cycle Checkpoints
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2016
- Paginierung
- 856 - 863
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Datum der Datenerfassung
- 2016
- Titel
- Cytotoxicity of seven naturally occurring phenolic compounds towards multi-factorial drug-resistant cancer cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 23
Data source: Europe PubMed Central
- Abstract
- INTRODUCTION: In medical oncology, multi-drug resistance (MDR) of cancer cells continues to be a major impediment. We are in quest of novel anti-proliferative agents to overcome drug-resistant tumor cells. METHODS: In the present study, we investigated the cytotoxicity of 7 naturally occurring phenolic compounds including two isoflavonoids alpinumisoflavone (1) and laburnetin (2), one biflavonoid amentoflavone (3), three lignans pycnanthulignene A (4), pycnanthulignene B (5), and syringaresinol (7) and one xanthone, euxanthone (6) against 9 drug-sensitive and MDR cancer cell lines. The resazurin reduction assay was used to evaluate the cytotoxicity of these compounds, whilst caspase-Glo assay was used to detect caspase activation. Cell cycle, mitochondrial membrane potential (MMP) and levels of reactive oxygen species (ROS) were all analyzed via flow cytometry. RESULTS: The IC50 values for the investigational phenolics ranged from 5.91 µM (towards leukemia CEM/ADR5000 cells) to 65.65 µM (towards drug-resistant breast adenocarcinoma MDA-MB-231-BCRP cells) for 1, 27.63 µM (towards leukemia CCRF-CEM cells) to 107.57 µM (towards MDA-MB-231-pcDNA cells) for 2, from 5.84 µM (towards CEM/ADR5000 cells) to 65.32 µM (towards colon carcinoma HCT116 (p53(-/-)) cells) for 4 and 0.20 µM (towards CCRF-CEM cells) to 195.12 µM (towards leukemia CEM/ADR5000) for doxorubicin. Phenolics 3, 5, 6 and 7 displayed selectivity cytotoxic effects on cancer cells lines. Compounds 1 and 4 induced apoptosis in CCRF-CEM cells, mediated by loss of MMP and increase ROS production. CONCLUSIONS: The studied phenolics and mostly isoflavonoid 1 and lignan 4 are potential cytotoxic natural products that deserve more investigations to develop novel antineoplastic drugs against multifactorial drug-resistant cancers.
- Date of acceptance
- 2016
- Autoren
- Victor Kuete
- Armelle T Mbaveng
- Eric CN Nono
- Christophe C Simo
- Maen Zeino
- Augustin E Nkengfack
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/27288921
- DOI
- 10.1016/j.phymed.2016.04.007
- eISSN
- 1618-095X
- Ausgabe der Veröffentlichung
- 8
- Zeitschrift
- Phytomedicine
- Schlüsselwörter
- Alpinumisoflavone
- Apoptosis
- Cytotoxicity
- Multi-drug resistance
- Phenolic compounds
- Pycnanthulignene
- Antineoplastic Agents, Phytogenic
- Caspases
- Cell Cycle Checkpoints
- Cell Line, Tumor
- Drug Resistance, Multiple
- Drug Resistance, Neoplasm
- Enzyme Activation
- Humans
- Membrane Potential, Mitochondrial
- Neoplasms
- Oxazines
- Phenols
- Reactive Oxygen Species
- Xanthenes
- Sprache
- eng
- Country
- Germany
- Paginierung
- 856 - 863
- PII
- S0944-7113(16)30045-9
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2017
- Titel
- Cytotoxicity of seven naturally occurring phenolic compounds towards multi-factorial drug-resistant cancer cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 23
Data source: PubMed
- Beziehungen:
- Property of