Efficient extraction and preparative separation of four main isoflavonoids from Dalbergia odorifera T. Chen leaves by deep eutectic solvents-based negative pressure cavitation extraction followed by macroporous resin column chromatography

Autoren

Lu Li
Ju-Zhao Liu
Meng Luo
Wei Wang
Yu-Yan Huang
Thomas Efferth
Hui-Mei Wang
Yu-Jie Fu

DOI

10.1016/j.jchromb.2016.08.005

ISSN

1570-0232

Zeitschrift

Journal of Chromatography B

Sprache

en

Paginierung

40 - 48

Datum der Veröffentlichung

2016

Status

Published

Herausgeber

Elsevier BV

Herausgeber URL

http://dx.doi.org/10.1016/j.jchromb.2016.08.005

Datum der Datenerfassung

2020

Titel

Efficient extraction and preparative separation of four main isoflavonoids from Dalbergia odorifera T. Chen leaves by deep eutectic solvents-based negative pressure cavitation extraction followed by macroporous resin column chromatography

Ausgabe der Zeitschrift

1033-1034

Data source: Crossref

Abstract

In this study, green and efficient deep eutectic solvent-based negative pressure cavitation-assisted extraction (DES-NPCE) followed by macroporous resin column chromatography was developed to extract and separate four main isoflavonoids, i.e. prunetin, tectorigenin, genistein and biochanin A from Dalbergia odorifera T. Chen leaves. The extraction procedure was optimized systematically by single-factor experiments and a Box-Behnken experimental design combined with response surface methodology. The maximum extraction yields of prunetin, tectorigenin, genistein and biochanin A reached 1.204, 1.057, 0.911 and 2.448mg/g dry weight, respectively. Moreover, the direct enrichment and separation of four isoflavonoids in DES extraction solution was successfully achieved by macroporous resin AB-8 with recovery yields of more than 80%. The present study provides a convenient and efficient method for the green extraction and preparative separation of active compounds from plants.

Addresses

Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China.

Autoren

Lu Li
Ju-Zhao Liu
Meng Luo
Wei Wang
Yu-Yan Huang
Thomas Efferth
Thomas Efferth
Hui-Mei Wang
Yu-Jie Fu

DOI

10.1016/j.jchromb.2016.08.005

eISSN

1873-376X

Externe Identifier

PubMed Identifier: 27517524

Funding acknowledgements

Application Technology Research and Development Program of Harbin: 2013AA3BS014
Special Fund of National Natural Science Foundation of China: 31270618
Fundamental Research Funds for the Central Universities: 2572015EA04
National Key Research Development Program of China: 2016YFD0600805

Open access

false

ISSN

1570-0232

Zeitschrift

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Schlüsselwörter

Dalbergia
Plant Leaves
Isoflavones
Plant Extracts
Chromatography, Liquid

Sprache

eng

Medium

Print-Electronic

Online publication date

2016

Paginierung

40 - 48

Datum der Veröffentlichung

2016

Status

Published

Datum der Datenerfassung

2016

Titel

Efficient extraction and preparative separation of four main isoflavonoids from Dalbergia odorifera T. Chen leaves by deep eutectic solvents-based negative pressure cavitation extraction followed by macroporous resin column chromatography.

Sub types

Journal Article

Ausgabe der Zeitschrift

1033-1034

Data source: Europe PubMed Central

Abstract

In this study, green and efficient deep eutectic solvent-based negative pressure cavitation-assisted extraction (DES-NPCE) followed by macroporous resin column chromatography was developed to extract and separate four main isoflavonoids, i.e. prunetin, tectorigenin, genistein and biochanin A from Dalbergia odorifera T. Chen leaves. The extraction procedure was optimized systematically by single-factor experiments and a Box-Behnken experimental design combined with response surface methodology. The maximum extraction yields of prunetin, tectorigenin, genistein and biochanin A reached 1.204, 1.057, 0.911 and 2.448mg/g dry weight, respectively. Moreover, the direct enrichment and separation of four isoflavonoids in DES extraction solution was successfully achieved by macroporous resin AB-8 with recovery yields of more than 80%. The present study provides a convenient and efficient method for the green extraction and preparative separation of active compounds from plants.

Date of acceptance

2016

Autoren

Lu Li
Ju-Zhao Liu
Meng Luo
Wei Wang
Yu-Yan Huang
Thomas Efferth
Hui-Mei Wang
Yu-Jie Fu

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/27517524

DOI

10.1016/j.jchromb.2016.08.005

eISSN

1873-376X

Zeitschrift

J Chromatogr B Analyt Technol Biomed Life Sci

Schlüsselwörter

Dalbergia odorifera T. Chen
Deep eutectic solvent
Isoflavonoids
Macroporous resin column chromatography
Negative pressure cavitation-assisted extraction
Chromatography, Liquid
Dalbergia
Isoflavones
Plant Extracts
Plant Leaves

Sprache

eng

Country

Netherlands

Paginierung

40 - 48

PII

S1570-0232(16)30561-X

Datum der Veröffentlichung

2016

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2017

Titel

Efficient extraction and preparative separation of four main isoflavonoids from Dalbergia odorifera T. Chen leaves by deep eutectic solvents-based negative pressure cavitation extraction followed by macroporous resin column chromatography.

Sub types

Journal Article

Ausgabe der Zeitschrift

1033-1034

Data source: PubMed

Efficient extraction and preparative separation of four main isoflavonoids from Dalbergia odorifera T. Chen leaves by deep eutectic solvents-based negative pressure cavitation extraction followed by macroporous resin column chromatography

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