Cytotoxicity of methanol extracts of 10 Cameroonian medicinal plants towards multi-factorial drug-resistant cancer cell lines
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Victor Kuete
- Cedric F Tchinda
- Flora T Mambe
- Veronique P Beng
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000381563100001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1186/s12906-016-1253-3
- Externe Identifier
- Clarivate Analytics Document Solution ID: DT5ZV
- PubMed Identifier: 27484842
- ISSN
- 1472-6882
- Zeitschrift
- BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
- Schlüsselwörter
- Albizia adiathifolia
- Alchornea cordifolia
- Apoptosis
- Cameroon
- Cancer
- Cytotoxicity
- Medicinal plants
- Multidrug resistance
- Artikelnummer
- ARTN 267
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Titel
- Cytotoxicity of methanol extracts of 10 Cameroonian medicinal plants towards multi-factorial drug-resistant cancer cell lines
- Sub types
- Article
- Ausgabe der Zeitschrift
- 16
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Victor Kuete
- Cedric F Tchinda
- Flora T Mambe
- Veronique P Beng
- Thomas Efferth
- DOI
- 10.1186/s12906-016-1253-3
- eISSN
- 1472-6882
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- BMC Complementary and Alternative Medicine
- Sprache
- en
- Artikelnummer
- 267
- Online publication date
- 2016
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1186/s12906-016-1253-3
- Datum der Datenerfassung
- 2019
- Titel
- Cytotoxicity of methanol extracts of 10 Cameroonian medicinal plants towards multi-factorial drug-resistant cancer cell lines
- Ausgabe der Zeitschrift
- 16
Data source: Crossref
- Abstract
- <h4>Background</h4>Cancer chemotherapy is still hampered by clinical failures due to multi-drug resistance (MDR) of tumor cells. In the present study, we have investigated the cytotoxicity of 20 methanol extracts from 10 medicinal plants against the sensitive leukemia CCRF-CEM cells. The most cytotoxic extracts were then further tested on a panel of 8 human cancer cell lines, including various MDR phenotypes.<h4>Methods</h4>The cytotoxicity of the 20 methanol extracts from 10 Cameroonian medicinal plants was determined using a resazurin reduction assay. Meanwhile, flow cytometry was used to measure cell cycle, apoptosis, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS).<h4>Results</h4>In the preliminary assay using CCRF-CEM cells, 12 extracts from five plants displayed IC50 values below 80 μg/mL, namely Albizia adianthifolia, Alchornea cordifolia, Alchornea laxiflora, Pennisetum purpureum, and Spathodea campanulata. the four best extracts were from two plants: Albizia adianthifolia roots (AAR) and bark (AAB) as well as Alchornea cordifolia leaves (ACL) and bark (ACB) had respective IC50 values of 0.98 μg/mL, 1.45 μg/mL, 8.02 μg/mL and 12.57 μg/mL in CCRF-CEM cells. They were further tested in 8 other cell lines as well as in normal AML12 hepatocytes. IC50 values ranging from 2.71 μg/mL (towards glioblastoma U87MG.ΔEGFR cells) to 10.30 μg/mL (towards breast adenocarcinoma MDA-MB-231-BCRP cells) for AAB, from 3.43 μg/mL (towards U87MG cells) to 10.77 μg/mL (towards colon carcinoma HCT116 (p53 (-/-) ) cells) for AAR and from 0.11 μg/mL (towards CCRF-CEM cells) to 108 μg/mL (towards leukemia CEM/ADR5000 cells) for doxorubicin (as control drug) were obtained. ACL and ACB extracts displayed selective activities. AAR and ACL extracts induced apoptosis in CCRF-CEM cells, through caspases activation and loss of MMP, while apoptotic cell death was mediated by MMP diruption and increase ROS production for ACL.<h4>Conclusion</h4>Some of the tested plants namely Albizia adianthifolia, Alchornea cordifolia, Alchornea laxiflora, Pennisetum purpureum, Spathodea campanulata represent a potential source of novel anticancer drugs. Especially, Albizia adianthifolia and Alchornea cordifolia revealed considerable cytotoxic activities that could be exploited to develop phytomedicines to fight cancers including MDR phenotypes.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, 55128, Mainz, Germany. kuetevictor@yahoo.fr.
- Autoren
- Victor Kuete
- Cedric F Tchinda
- Flora T Mambe
- Veronique P Beng
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1186/s12906-016-1253-3
- eISSN
- 1472-6882
- Externe Identifier
- PubMed Identifier: 27484842
- PubMed Central ID: PMC4970256
- Funding acknowledgements
- Alexander von Humboldt-Stiftung: 2015-2018 Linkage program
- Open access
- true
- ISSN
- 1472-6882
- Zeitschrift
- BMC complementary and alternative medicine
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Plants, Medicinal
- Caspases
- Plant Extracts
- Antineoplastic Agents, Phytogenic
- Drug Resistance, Multiple
- Cell Cycle
- Apoptosis
- Cell Proliferation
- Drug Resistance, Neoplasm
- Cameroon
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2016
- Open access status
- Open Access
- Paginierung
- 267
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2016
- Titel
- Cytotoxicity of methanol extracts of 10 Cameroonian medicinal plants towards multi-factorial drug-resistant cancer cell lines.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 16
Files
https://bmccomplementalternmed.biomedcentral.com/track/pdf/10.1186/s12906-016-1253-3 https://europepmc.org/articles/PMC4970256?pdf=render
Data source: Europe PubMed Central
- Abstract
- BACKGROUND: Cancer chemotherapy is still hampered by clinical failures due to multi-drug resistance (MDR) of tumor cells. In the present study, we have investigated the cytotoxicity of 20 methanol extracts from 10 medicinal plants against the sensitive leukemia CCRF-CEM cells. The most cytotoxic extracts were then further tested on a panel of 8 human cancer cell lines, including various MDR phenotypes. METHODS: The cytotoxicity of the 20 methanol extracts from 10 Cameroonian medicinal plants was determined using a resazurin reduction assay. Meanwhile, flow cytometry was used to measure cell cycle, apoptosis, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS). RESULTS: In the preliminary assay using CCRF-CEM cells, 12 extracts from five plants displayed IC50 values below 80 μg/mL, namely Albizia adianthifolia, Alchornea cordifolia, Alchornea laxiflora, Pennisetum purpureum, and Spathodea campanulata. the four best extracts were from two plants: Albizia adianthifolia roots (AAR) and bark (AAB) as well as Alchornea cordifolia leaves (ACL) and bark (ACB) had respective IC50 values of 0.98 μg/mL, 1.45 μg/mL, 8.02 μg/mL and 12.57 μg/mL in CCRF-CEM cells. They were further tested in 8 other cell lines as well as in normal AML12 hepatocytes. IC50 values ranging from 2.71 μg/mL (towards glioblastoma U87MG.ΔEGFR cells) to 10.30 μg/mL (towards breast adenocarcinoma MDA-MB-231-BCRP cells) for AAB, from 3.43 μg/mL (towards U87MG cells) to 10.77 μg/mL (towards colon carcinoma HCT116 (p53 (-/-) ) cells) for AAR and from 0.11 μg/mL (towards CCRF-CEM cells) to 108 μg/mL (towards leukemia CEM/ADR5000 cells) for doxorubicin (as control drug) were obtained. ACL and ACB extracts displayed selective activities. AAR and ACL extracts induced apoptosis in CCRF-CEM cells, through caspases activation and loss of MMP, while apoptotic cell death was mediated by MMP diruption and increase ROS production for ACL. CONCLUSION: Some of the tested plants namely Albizia adianthifolia, Alchornea cordifolia, Alchornea laxiflora, Pennisetum purpureum, Spathodea campanulata represent a potential source of novel anticancer drugs. Especially, Albizia adianthifolia and Alchornea cordifolia revealed considerable cytotoxic activities that could be exploited to develop phytomedicines to fight cancers including MDR phenotypes.
- Date of acceptance
- 2016
- Autoren
- Victor Kuete
- Cedric F Tchinda
- Flora T Mambe
- Veronique P Beng
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/27484842
- DOI
- 10.1186/s12906-016-1253-3
- eISSN
- 1472-6882
- Externe Identifier
- PubMed Central ID: PMC4970256
- Zeitschrift
- BMC Complement Altern Med
- Schlüsselwörter
- Albizia adiathifolia
- Alchornea cordifolia
- Apoptosis
- Cameroon
- Cancer
- Cytotoxicity
- Medicinal plants
- Multidrug resistance
- Antineoplastic Agents, Phytogenic
- Apoptosis
- Cameroon
- Caspases
- Cell Cycle
- Cell Line, Tumor
- Cell Proliferation
- Drug Resistance, Multiple
- Drug Resistance, Neoplasm
- Humans
- Plant Extracts
- Plants, Medicinal
- Sprache
- eng
- Country
- England
- Paginierung
- 267
- PII
- 10.1186/s12906-016-1253-3
- Datum der Veröffentlichung
- 2016
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2017
- Titel
- Cytotoxicity of methanol extracts of 10 Cameroonian medicinal plants towards multi-factorial drug-resistant cancer cell lines.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 16
Data source: PubMed
- Beziehungen:
- Property of