Cytotoxicity of methanol extracts of Annona muricata, Passiflora edulis and nine other Cameroonian medicinal plants towards multi-factorial drug-resistant cancer cell lines
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Victor Kuete
- Joachim K Dzotam
- Igor K Voukeng
- Aime G Fankam
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000391802200004&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1186/s40064-016-3361-4
- Externe Identifier
- Clarivate Analytics Document Solution ID: EH5HC
- PubMed Identifier: 27730025
- ISSN
- 2193-1801
- Zeitschrift
- SPRINGERPLUS
- Schlüsselwörter
- Annona muricata
- Apoptosis
- Cameroon
- Cytotoxicity
- Medicinal plants
- Passiflora edulis
- Artikelnummer
- ARTN 1666
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Titel
- Cytotoxicity of methanol extracts of <i>Annona muricata</i>, <i>Passiflora edulis</i> and nine other Cameroonian medicinal plants towards multi-factorial drug-resistant cancer cell lines
- Sub types
- Article
- Ausgabe der Zeitschrift
- 5
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Victor Kuete
- Joachim K Dzotam
- Igor K Voukeng
- Aimé G Fankam
- Thomas Efferth
- DOI
- 10.1186/s40064-016-3361-4
- eISSN
- 2193-1801
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- SpringerPlus
- Sprache
- en
- Artikelnummer
- 1666
- Online publication date
- 2016
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1186/s40064-016-3361-4
- Datum der Datenerfassung
- 2023
- Titel
- Cytotoxicity of methanol extracts of Annona muricata, Passiflora edulis and nine other Cameroonian medicinal plants towards multi-factorial drug-resistant cancer cell lines
- Ausgabe der Zeitschrift
- 5
Data source: Crossref
- Abstract
- <h4>Background</h4>Cancer cells rapidly acquire resistance leading to treatment failures. In the present study, we have evaluated the cytotoxicity of 17 methanol extracts from 11 Cameroonian medicinal plants against the sensitive leukemia CCRF-CEM cells and the best ones were further tested on a panel of 8 other human cancer cell lines, including various MDR phenotypes as well as against the normal AML12 hepatocytes.<h4>Methods</h4>The cytotoxicity of the extracts was determined using a resazurin reduction assay meanwhile flow cytometry was used to measure cell cycle, apoptosis, mitochondrial membrane potential (MMP), and reactive oxygen species.<h4>Results</h4>In an initial screening using leukemia CCRF-CEM cells, ten extracts from five plants namely <i>Alchornea floribunda</i>, <i>Annona muricata</i>, <i>Euphorbia prostata</i>, <i>Pachypodanthium staudtii</i> and <i>Passiflora edulis</i> displayed IC<sub>50</sub> values below 20 µg/mL. They were further tested in 8 other cell lines as well as in normal AML12 hepatocytes. All selected extracts were active against leukemia CEM/ADR5000 cells with IC<sub>50</sub> value below 40 µg/mL. IC<sub>50</sub> values ranging from 10.13 µg/mL (towards CEM/ADR5000 cells) to 72.01 µg/mL [towards resistant colon carcinoma HCT116 (<i>p53</i><sup>-<i>/</i>-</sup>) cells] for <i>Pachypodanthium staudtii</i> roots and from 0.11 µg/mL (towards CCRF-CEM cells) to 108 µg/mL (towards P-glycoprotein-over-expressing CEM/ADR5000 cells) for doxorubicin were obtained in the eight other cancer cell lines studied. Extracts from <i>Annona muricata</i> leaves (AML) and seeds (AMS), and <i>Passiflora edulis</i> fruit (PEF) had IC<sub>50</sub> values below 1 µg/mL against CCRF-CEM cells and below 10 µg/mL against its MDR subline CEM/ADR5000 cells. AML, AMS and PEF induced MMP-loss-mediated apoptosis in CCRF-CEM cells.<h4>Conclusions</h4>Results of the present study suggest that some of the tested plants namely <i>Alchornea floribunda</i>, <i>Annona muricata</i>, <i>Euphorbia prostata</i>, <i>Pachypodanthium staudtii</i> and <i>Passiflora edulis</i> represent a source of anticancer drugs. <i>Annona muricata</i> and <i>Passiflora edulis</i> are good cytotoxic plants that could be exploited to develop phytomedicine to fight mostly hematological cancers including MDR phenotypes.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, 55128 Mainz, Germany ; Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
- Autoren
- Victor Kuete
- Joachim K Dzotam
- Igor K Voukeng
- Aimé G Fankam
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1186/s40064-016-3361-4
- eISSN
- 2193-1801
- Externe Identifier
- PubMed Identifier: 27730025
- PubMed Central ID: PMC5039145
- Funding acknowledgements
- Alexander von Humboldt-Stiftung: 2015-2018 Linkage program
- Open access
- true
- ISSN
- 2193-1801
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- SpringerPlus
- Sprache
- eng
- Medium
- Electronic-eCollection
- Online publication date
- 2016
- Open access status
- Open Access
- Paginierung
- 1666
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2016
- Titel
- Cytotoxicity of methanol extracts of <i>Annona muricata</i>, <i>Passiflora edulis</i> and nine other Cameroonian medicinal plants towards multi-factorial drug-resistant cancer cell lines.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 5
Files
https://springerplus.springeropen.com/track/pdf/10.1186/s40064-016-3361-4 https://europepmc.org/articles/PMC5039145?pdf=render
Data source: Europe PubMed Central
- Abstract
- BACKGROUND: Cancer cells rapidly acquire resistance leading to treatment failures. In the present study, we have evaluated the cytotoxicity of 17 methanol extracts from 11 Cameroonian medicinal plants against the sensitive leukemia CCRF-CEM cells and the best ones were further tested on a panel of 8 other human cancer cell lines, including various MDR phenotypes as well as against the normal AML12 hepatocytes. METHODS: The cytotoxicity of the extracts was determined using a resazurin reduction assay meanwhile flow cytometry was used to measure cell cycle, apoptosis, mitochondrial membrane potential (MMP), and reactive oxygen species. RESULTS: In an initial screening using leukemia CCRF-CEM cells, ten extracts from five plants namely Alchornea floribunda, Annona muricata, Euphorbia prostata, Pachypodanthium staudtii and Passiflora edulis displayed IC50 values below 20 µg/mL. They were further tested in 8 other cell lines as well as in normal AML12 hepatocytes. All selected extracts were active against leukemia CEM/ADR5000 cells with IC50 value below 40 µg/mL. IC50 values ranging from 10.13 µg/mL (towards CEM/ADR5000 cells) to 72.01 µg/mL [towards resistant colon carcinoma HCT116 (p53-/-) cells] for Pachypodanthium staudtii roots and from 0.11 µg/mL (towards CCRF-CEM cells) to 108 µg/mL (towards P-glycoprotein-over-expressing CEM/ADR5000 cells) for doxorubicin were obtained in the eight other cancer cell lines studied. Extracts from Annona muricata leaves (AML) and seeds (AMS), and Passiflora edulis fruit (PEF) had IC50 values below 1 µg/mL against CCRF-CEM cells and below 10 µg/mL against its MDR subline CEM/ADR5000 cells. AML, AMS and PEF induced MMP-loss-mediated apoptosis in CCRF-CEM cells. CONCLUSIONS: Results of the present study suggest that some of the tested plants namely Alchornea floribunda, Annona muricata, Euphorbia prostata, Pachypodanthium staudtii and Passiflora edulis represent a source of anticancer drugs. Annona muricata and Passiflora edulis are good cytotoxic plants that could be exploited to develop phytomedicine to fight mostly hematological cancers including MDR phenotypes.
- Date of acceptance
- 2016
- Autoren
- Victor Kuete
- Joachim K Dzotam
- Igor K Voukeng
- Aimé G Fankam
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/27730025
- DOI
- 10.1186/s40064-016-3361-4
- Externe Identifier
- PubMed Central ID: PMC5039145
- ISSN
- 2193-1801
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Springerplus
- Schlüsselwörter
- Annona muricata
- Apoptosis
- Cameroon
- Cytotoxicity
- Medicinal plants
- Passiflora edulis
- Sprache
- eng
- Country
- Switzerland
- Paginierung
- 1666
- PII
- 3361
- Datum der Veröffentlichung
- 2016
- Status
- Published online
- Titel
- Cytotoxicity of methanol extracts of Annona muricata, Passiflora edulis and nine other Cameroonian medicinal plants towards multi-factorial drug-resistant cancer cell lines.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 5
Data source: PubMed
- Beziehungen:
- Property of