Anti-inflammatory Effects of Herbal Preparations STW5 and STW5-II in Cytokine-Challenged Normal Human Colon Cells

Publication type:
Journal article
Metadata:
Autoren
  • Mathias Schneider
  • Thomas Efferth
  • Heba Abdel-Aziz
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000386168200001&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.3389/fphar.2016.00393
Externe Identifier
  • Clarivate Analytics Document Solution ID: DZ9BM
  • PubMed Identifier: 27833553
ISSN
1663-9812
Zeitschrift
FRONTIERS IN PHARMACOLOGY
Schlüsselwörter
  • Inflammatory bowel disease
  • Phytotherapy
  • Proteomics
Artikelnummer
ARTN 393
Datum der Veröffentlichung
2016
Status
Published
Titel
Anti-inflammatory Effects of Herbal Preparations STW5 and STW5-II in Cytokine-Challenged Normal Human Colon Cells
Sub types
  • Article
Ausgabe der Zeitschrift
7

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Mathias Schneider
  • Thomas Efferth
  • Heba Abdel-Aziz
DOI
10.3389/fphar.2016.00393
eISSN
1663-9812
Zeitschrift
Frontiers in Pharmacology
Online publication date
2016
Status
Published online
Herausgeber
Frontiers Media SA
Herausgeber URL
http://dx.doi.org/10.3389/fphar.2016.00393
Datum der Datenerfassung
2016
Titel
Anti-inflammatory Effects of Herbal Preparations STW5 and STW5-II in Cytokine-Challenged Normal Human Colon Cells
Ausgabe der Zeitschrift
7

Data source: Crossref

Abstract
Inflammatory bowel diseases (IBD) are chronic relapsing intestinal disorders characterized by up-regulation of pro-inflammatory cytokines followed by invasion of immune cells to the intestinal lamina propria. Standard therapies consist of anti-inflammatory or immunosuppressive drugs. Since clinical efficiency is not satisfactory and the established drugs have massive side effects, new strategies to treat IBD are required. Herein, we investigate the protective effect of the fixed combination herbal preparations STW5 and STW5-II and the contribution of the corresponding single components in an <i>in vitro</i> inflammation model. The normal human colon epithelial cell line, NCM460, was treated with STW5, STW5-II or their single components for 4 h followed by experimental conditions comparable to induction of colitis. A pro-inflammatory cytokine cocktail consisting of TNF-α, IL-β, and IFN-γ was used to simulate inflammatory stimuli normally caused by immune cells. The effects on NCM460 cells were investigated by enzyme-linked immunoassay and Proteome Profiler<sup>®</sup>. Levels of IP-10, MCP-1, I-TAC, Groα, and IL-8 were elevated in chemokine-treated cells compared to untreated cells, but significantly reduced upon pretreatment with STW5 or STW5-II. However, the single compounds revealed only little effects on protein expression. Furthermore, we investigated the effect of both combination preparations on pro-inflammatory transcription factors of the STAT family using Western blot. In addition, we tested the effects on upstream MAPK p38. Both, STW5 and STW5-II did not show any effect on MAPK p38, but were effective in reducing phosphorylated levels of STAT1. In conclusion, both combination preparations act in an anti-inflammatory manner by influencing cytokine secretion via reduced activity of the JAK/STAT1 pathway. Relevant differences between STW5 and STW5-II were not found indicating similar efficacies.
Addresses
  • Department of Pharmaceutical Biology, Johannes Gutenberg University, Mainz Germany.
Autoren
DOI
10.3389/fphar.2016.00393
eISSN
1663-9812
Externe Identifier
  • PubMed Identifier: 27833553
  • PubMed Central ID: PMC5080345
Open access
true
ISSN
1663-9812
Zeitschrift
Frontiers in pharmacology
Sprache
eng
Medium
Electronic-eCollection
Online publication date
2016
Open access status
Open Access
Paginierung
393
Datum der Veröffentlichung
2016
Status
Published
Publisher licence
CC BY
Datum der Datenerfassung
2016
Titel
Anti-inflammatory Effects of Herbal Preparations STW5 and STW5-II in Cytokine-Challenged Normal Human Colon Cells.
Sub types
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
7

Files

https://www.frontiersin.org/articles/10.3389/fphar.2016.00393/pdf https://europepmc.org/articles/PMC5080345?pdf=render

Data source: Europe PubMed Central

Abstract
Inflammatory bowel diseases (IBD) are chronic relapsing intestinal disorders characterized by up-regulation of pro-inflammatory cytokines followed by invasion of immune cells to the intestinal lamina propria. Standard therapies consist of anti-inflammatory or immunosuppressive drugs. Since clinical efficiency is not satisfactory and the established drugs have massive side effects, new strategies to treat IBD are required. Herein, we investigate the protective effect of the fixed combination herbal preparations STW5 and STW5-II and the contribution of the corresponding single components in an in vitro inflammation model. The normal human colon epithelial cell line, NCM460, was treated with STW5, STW5-II or their single components for 4 h followed by experimental conditions comparable to induction of colitis. A pro-inflammatory cytokine cocktail consisting of TNF-α, IL-β, and IFN-γ was used to simulate inflammatory stimuli normally caused by immune cells. The effects on NCM460 cells were investigated by enzyme-linked immunoassay and Proteome Profiler®. Levels of IP-10, MCP-1, I-TAC, Groα, and IL-8 were elevated in chemokine-treated cells compared to untreated cells, but significantly reduced upon pretreatment with STW5 or STW5-II. However, the single compounds revealed only little effects on protein expression. Furthermore, we investigated the effect of both combination preparations on pro-inflammatory transcription factors of the STAT family using Western blot. In addition, we tested the effects on upstream MAPK p38. Both, STW5 and STW5-II did not show any effect on MAPK p38, but were effective in reducing phosphorylated levels of STAT1. In conclusion, both combination preparations act in an anti-inflammatory manner by influencing cytokine secretion via reduced activity of the JAK/STAT1 pathway. Relevant differences between STW5 and STW5-II were not found indicating similar efficacies.
Date of acceptance
2016
Autoren
  • Mathias Schneider
  • Thomas Efferth
  • Heba Abdel-Aziz
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/27833553
DOI
10.3389/fphar.2016.00393
Externe Identifier
  • PubMed Central ID: PMC5080345
ISSN
1663-9812
Zeitschrift
Front Pharmacol
Schlüsselwörter
  • Inflammatory bowel disease
  • Phytotherapy
  • Proteomics
Sprache
eng
Country
Switzerland
Paginierung
393
Datum der Veröffentlichung
2016
Status
Published online
Titel
Anti-inflammatory Effects of Herbal Preparations STW5 and STW5-II in Cytokine-Challenged Normal Human Colon Cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
7

Data source: PubMed

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