Ziziphora tenuior L. essential oil from Dana Biosphere Reserve (Southern Jordan); Chemical characterization and assessment of biological activities

Publication type:
Journal article
Metadata:
Autoren
  • MS Abu-Darwish
  • C Cabral
  • MJ Goncalves
  • C Cavaleiro
  • MT Cruz
  • M Paoli
  • F Tomi
  • T Efferth
  • L Salgueiro
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000391076800101&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.jep.2016.10.076
Externe Identifier
  • Clarivate Analytics Document Solution ID: EG5IF
  • PubMed Identifier: 27815078
ISSN
0378-8741
Zeitschrift
JOURNAL OF ETHNOPHARMACOLOGY
Schlüsselwörter
  • Ziziphora tenuior
  • Essential oil
  • Chemical composition
  • Antifungal activity
  • Anti-inflammatory activity
  • Cytotoxicity
Paginierung
963 - 970
Datum der Veröffentlichung
2016
Status
Published
Titel
<i>Ziziphora tenuior</i> L. essential oil from Dana Biosphere Reserve (Southern Jordan); Chemical characterization and assessment of biological activities
Sub types
  • Article
Ausgabe der Zeitschrift
194

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • MS Abu-Darwish
  • C Cabral
  • MJ Gonçalves
  • C Cavaleiro
  • MT Cruz
  • M Paoli
  • F Tomi
  • T Efferth
  • L Salgueiro
DOI
10.1016/j.jep.2016.10.076
ISSN
0378-8741
Zeitschrift
Journal of Ethnopharmacology
Sprache
en
Paginierung
963 - 970
Datum der Veröffentlichung
2016
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.jep.2016.10.076
Datum der Datenerfassung
2019
Titel
Ziziphora tenuior L. essential oil from Dana Biosphere Reserve (Southern Jordan); Chemical characterization and assessment of biological activities
Ausgabe der Zeitschrift
194

Data source: Crossref

Abstract
<h4>Ethnopharmacologic relevance</h4>Ziziphora tenuior L. (Lamiaceae) is a medicinal plant in Jordan, which is included in various antimicrobial, antiseptic, expectorant and wound healing preparations. It is used for the treatment of cough, stomach ache, dysentery, fever, uterus infection, gut inflammation and painful menstruation.<h4>Aim of the study</h4>The aim of this study was to assess, for the first time, the chemical composition of the essential oil of Z. tenuior originated from southern Jordan and its antifungal effects against several yeasts. Concomitantly, the mechanisms behind the anti-fungal activity against Candida albicans were also disclosed. Since the Z. tenuior traditional uses are related with inflammatory-associated conditions, the putative anti-inflammatory activity of the oil was also unveiled. Importantly, the potential toxicity of pharmacologically active concentrations was screened in different types of mammalian cells.<h4>Materials and methods</h4>Z. tenuior essential oil, isolated by hydrodistillation, was analyzed by gas chromatography, gas chromatography-mass spectrometry and <sup>13</sup>C nuclear magnetic resonance spectroscopy. Antifungal activity was evaluated against yeasts, dermatophytes and Aspergillus strains. Germ tube inhibition and biofilm formation assays were evaluated using C. albicans. Assessment of cell viability was made by the MTT assay using different types of mammalian cells, including hepatocytes, keratinocytes and macrophages. The in vitro anti-inflammatory potential of the oil was evaluated by measuring nitric oxide production using lipopolysaccharide-stimulated mouse macrophages.<h4>Results</h4>Oxygen-containing monoterpenes are the main oil compounds: pulegone (46.8%), p-menth-3-en-8-ol (12.5%), isomenthone (6.6%) and 8-hydroxymenthone (6.2%). The highest antifungal activity was against Cryptococcus neoformans, with a MIC value of 0.16µL/mL. The oil revealed an important inhibitory effect on germ tube formation with a filamentation inhibition rate higher than 80% at 0.16µL/mL. The amount of the attached biomass was reduced. Importantly, concentrations devoid of toxicity on several mammalian cell types still displayed anti-inflammatory activity (0.16 and 0.32µL/mL).<h4>Conclusions</h4>These findings add significant information to the pharmacological activity of Z. tenuior, thus justifying and reinforcing the use of this plant in traditional medicine. Additionally, the antifungal and anti-inflammatory potential of the oil at non-toxic concentrations, opens new avenues for its further exploitation, for instance in health-care product development.
Addresses
  • Department of Basic and Applied Sciences, Shouback University College/ Maan College, Al-Balqa Applied University, Al-Salt 19117, Jordan; Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Staudinger Weg 5, 55128 Mainz, Germany.
Autoren
  • MS Abu-Darwish
  • C Cabral
  • MJ Gonçalves
  • C Cavaleiro
  • MT Cruz
  • M Paoli
  • F Tomi
  • T Efferth
  • T Efferth
  • L Salgueiro
DOI
10.1016/j.jep.2016.10.076
eISSN
1872-7573
Externe Identifier
  • PubMed Identifier: 27815078
Funding acknowledgements
  • Post-Doc fellowship: SFRH/BPD/68481/2010
  • FCT – Fundação para a Ciência e a Tecnologia, Portugal: C/SAU/LA0001/2013–2014
Open access
false
ISSN
0378-8741
Zeitschrift
Journal of ethnopharmacology
Schlüsselwörter
  • Cell Line
  • Cell Line, Tumor
  • Macrophages
  • Animals
  • Humans
  • Mice
  • Aspergillus
  • Candida albicans
  • Cryptococcus neoformans
  • Lamiaceae
  • Terpenes
  • Monoterpenes
  • Oils, Volatile
  • Plant Oils
  • Anti-Inflammatory Agents
  • Antifungal Agents
  • Microbial Sensitivity Tests
  • Biomass
  • Jordan
  • Hep G2 Cells
  • Cyclohexane Monoterpenes
Sprache
eng
Medium
Print-Electronic
Online publication date
2016
Paginierung
963 - 970
Datum der Veröffentlichung
2016
Status
Published
Datum der Datenerfassung
2016
Titel
Ziziphora tenuior L. essential oil from Dana Biosphere Reserve (Southern Jordan); Chemical characterization and assessment of biological activities.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
194

Data source: Europe PubMed Central

Abstract
ETHNOPHARMACOLOGIC RELEVANCE: Ziziphora tenuior L. (Lamiaceae) is a medicinal plant in Jordan, which is included in various antimicrobial, antiseptic, expectorant and wound healing preparations. It is used for the treatment of cough, stomach ache, dysentery, fever, uterus infection, gut inflammation and painful menstruation. AIM OF THE STUDY: The aim of this study was to assess, for the first time, the chemical composition of the essential oil of Z. tenuior originated from southern Jordan and its antifungal effects against several yeasts. Concomitantly, the mechanisms behind the anti-fungal activity against Candida albicans were also disclosed. Since the Z. tenuior traditional uses are related with inflammatory-associated conditions, the putative anti-inflammatory activity of the oil was also unveiled. Importantly, the potential toxicity of pharmacologically active concentrations was screened in different types of mammalian cells. MATERIALS AND METHODS: Z. tenuior essential oil, isolated by hydrodistillation, was analyzed by gas chromatography, gas chromatography-mass spectrometry and 13C nuclear magnetic resonance spectroscopy. Antifungal activity was evaluated against yeasts, dermatophytes and Aspergillus strains. Germ tube inhibition and biofilm formation assays were evaluated using C. albicans. Assessment of cell viability was made by the MTT assay using different types of mammalian cells, including hepatocytes, keratinocytes and macrophages. The in vitro anti-inflammatory potential of the oil was evaluated by measuring nitric oxide production using lipopolysaccharide-stimulated mouse macrophages. RESULTS: Oxygen-containing monoterpenes are the main oil compounds: pulegone (46.8%), p-menth-3-en-8-ol (12.5%), isomenthone (6.6%) and 8-hydroxymenthone (6.2%). The highest antifungal activity was against Cryptococcus neoformans, with a MIC value of 0.16µL/mL. The oil revealed an important inhibitory effect on germ tube formation with a filamentation inhibition rate higher than 80% at 0.16µL/mL. The amount of the attached biomass was reduced. Importantly, concentrations devoid of toxicity on several mammalian cell types still displayed anti-inflammatory activity (0.16 and 0.32µL/mL). CONCLUSIONS: These findings add significant information to the pharmacological activity of Z. tenuior, thus justifying and reinforcing the use of this plant in traditional medicine. Additionally, the antifungal and anti-inflammatory potential of the oil at non-toxic concentrations, opens new avenues for its further exploitation, for instance in health-care product development.
Date of acceptance
2016
Autoren
  • MS Abu-Darwish
  • C Cabral
  • MJ Gonçalves
  • C Cavaleiro
  • MT Cruz
  • M Paoli
  • F Tomi
  • T Efferth
  • L Salgueiro
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/27815078
DOI
10.1016/j.jep.2016.10.076
eISSN
1872-7573
Zeitschrift
J Ethnopharmacol
Schlüsselwörter
  • Anti-inflammatory activity
  • Antifungal activity
  • Chemical composition
  • Cytotoxicity
  • Essential oil
  • Ziziphora tenuior
  • Animals
  • Anti-Inflammatory Agents
  • Antifungal Agents
  • Aspergillus
  • Biomass
  • Candida albicans
  • Cell Line
  • Cell Line, Tumor
  • Cryptococcus neoformans
  • Cyclohexane Monoterpenes
  • Hep G2 Cells
  • Humans
  • Jordan
  • Lamiaceae
  • Macrophages
  • Mice
  • Microbial Sensitivity Tests
  • Monoterpenes
  • Oils, Volatile
  • Plant Oils
  • Terpenes
Sprache
eng
Country
Ireland
Paginierung
963 - 970
PII
S0378-8741(16)31545-8
Datum der Veröffentlichung
2016
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2017
Titel
Ziziphora tenuior L. essential oil from Dana Biosphere Reserve (Southern Jordan); Chemical characterization and assessment of biological activities.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
194

Data source: PubMed

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