Total coumarins of Hedyotis diffusa induces apoptosis of myelodysplastic syndrome SKM-1 cells by activation of caspases and inhibition of PI3K/Akt pathway proteins
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Jianping Jiang
- Bo Wang
- Jing Li
- Baodong Ye
- Shengyun Lin
- Wenbin Qian
- Letian Shan
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000393529900027&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.jep.2016.12.012
- Externe Identifier
- Clarivate Analytics Document Solution ID: EJ9ER
- PubMed Identifier: 27988397
- ISSN
- 0378-8741
- Zeitschrift
- JOURNAL OF ETHNOPHARMACOLOGY
- Schlüsselwörter
- Apoptosis
- Traditional Chinese medicine
- Hedyotis diffuse
- Total coumarins
- Myelodysplastic syndromes
- SKM-1
- Paginierung
- 253 - 260
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Titel
- Total coumarins of <i>Hedyotis diffusa</i> induces apoptosis of myelodysplastic syndrome SKM-1 cells by activation of caspases and inhibition of PI3K/Akt pathway proteins
- Sub types
- Article
- Ausgabe der Zeitschrift
- 196
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Jianping Jiang
- Bo Wang
- Jing Li
- Baodong Ye
- Shengyun Lin
- Wenbin Qian
- Letian Shan
- Thomas Efferth
- DOI
- 10.1016/j.jep.2016.12.012
- ISSN
- 0378-8741
- Zeitschrift
- Journal of Ethnopharmacology
- Sprache
- en
- Paginierung
- 253 - 260
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.jep.2016.12.012
- Datum der Datenerfassung
- 2019
- Titel
- Total coumarins of Hedyotis diffusa induces apoptosis of myelodysplastic syndrome SKM-1 cells by activation of caspases and inhibition of PI3K/Akt pathway proteins
- Ausgabe der Zeitschrift
- 196
Data source: Crossref
- Abstract
- <h4>Ethnopharmacological relevance</h4>Hedyotis diffusa is an ethno-medicine used for anti-cancer treatment in the clinic of traditional Chinese medicine (TCM). The total coumarins of Hedyotis diffusa (TCHD) was a selected extract with observed antiproliferative activity, which has not been tested in treatment of myelodysplastic syndromes (MDS) or acute myeloid leukemia (AML).<h4>Aim of the study</h4>This study aimed to evaluate the apoptosis-inducing effect of TCHD on human MDS cell line (SKM-1) and explore its action mechanism in association with caspase family and PI3K/Akt signaling pathway.<h4>Materials and methods</h4>The chemical constituents and total coumarins content of TCHD were determined by High Performance Liquid Chromatography-tandem mass spectrometry (HPLC-MS/MS) and UV-vis spectrophotometry, respectively. MTT assay, Hoechst 33258 staining, and Annexin V-FITC/PI double labeling were applied to evaluate TCHD's efficacy on SKM-1 cells. Western blot analysis was also used to clarify the action mechanism of TCHD on protein expression level.<h4>Results</h4>Two compounds, p-coumaric acid and E-6-O-p-coumaroyl scandoside methyl ester, were identified in TCHD, and its total coumarins content reached 87.4%. By MTT assay, apoptosis-inducing effect of TCHD on SKM-1 cells was found in a dose-dependent manner after 24-48h treatment, with IC<sub>50</sub> values of 104.48μg/ml and 100.66μg/ml, respectively. Morphological and flow cytometry observation also confirmed such effect of TCHD. Western blot analysis clarified its action mechanism associating with the activation of caspases and inhibition of PI3K/Akt pathway proteins.<h4>Conclusions</h4>This is the first report regarding the apoptosis-inducing efficacy and mechanism of TCHD on SKM-1 cells, providing a promising candidate of TCM for MDS and AML therapy with fewer side effects.
- Addresses
- The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, PR China; Zhejiang Chinese Medical University, Hangzhou 310053, PR China.
- Autoren
- Jianping Jiang
- Bo Wang
- Jing Li
- Baodong Ye
- Shengyun Lin
- Wenbin Qian
- Letian Shan
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1016/j.jep.2016.12.012
- eISSN
- 1872-7573
- Externe Identifier
- PubMed Identifier: 27988397
- Funding acknowledgements
- Zhejiang Provincial Natural Science Foundation of China: LY13H290011
- Zhejiang Provincial Natural Science Foundation of China: LY14H080003
- Doctoral Program of Higher Education of China: 20133322120006
- National Natural Science Foundation of China: 81302989
- Zhejiang Provincial Natural Science Foundation of China: Y15H270031
- Hangzhou Medical Scientific Research Project: 20150633B59
- Zhejiang Provincial Science and Technology Project of Traditional Chinese Medicine of China: 2013ZQ007, 2015ZA074 and 2016ZZ011
- Open access
- false
- ISSN
- 0378-8741
- Zeitschrift
- Journal of ethnopharmacology
- Schlüsselwörter
- Cells, Cultured
- Cell Line
- Mesenchymal Stem Cells
- Humans
- Hedyotis
- Myelodysplastic Syndromes
- Coumarins
- Caspases
- Apoptosis
- Cell Proliferation
- Proto-Oncogene Proteins c-akt
- Phosphatidylinositol 3-Kinases
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2016
- Paginierung
- 253 - 260
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Datum der Datenerfassung
- 2016
- Titel
- Total coumarins of Hedyotis diffusa induces apoptosis of myelodysplastic syndrome SKM-1 cells by activation of caspases and inhibition of PI3K/Akt pathway proteins.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 196
Data source: Europe PubMed Central
- Abstract
- ETHNOPHARMACOLOGICAL RELEVANCE: Hedyotis diffusa is an ethno-medicine used for anti-cancer treatment in the clinic of traditional Chinese medicine (TCM). The total coumarins of Hedyotis diffusa (TCHD) was a selected extract with observed antiproliferative activity, which has not been tested in treatment of myelodysplastic syndromes (MDS) or acute myeloid leukemia (AML). AIM OF THE STUDY: This study aimed to evaluate the apoptosis-inducing effect of TCHD on human MDS cell line (SKM-1) and explore its action mechanism in association with caspase family and PI3K/Akt signaling pathway. MATERIALS AND METHODS: The chemical constituents and total coumarins content of TCHD were determined by High Performance Liquid Chromatography-tandem mass spectrometry (HPLC-MS/MS) and UV-vis spectrophotometry, respectively. MTT assay, Hoechst 33258 staining, and Annexin V-FITC/PI double labeling were applied to evaluate TCHD's efficacy on SKM-1 cells. Western blot analysis was also used to clarify the action mechanism of TCHD on protein expression level. RESULTS: Two compounds, p-coumaric acid and E-6-O-p-coumaroyl scandoside methyl ester, were identified in TCHD, and its total coumarins content reached 87.4%. By MTT assay, apoptosis-inducing effect of TCHD on SKM-1 cells was found in a dose-dependent manner after 24-48h treatment, with IC50 values of 104.48μg/ml and 100.66μg/ml, respectively. Morphological and flow cytometry observation also confirmed such effect of TCHD. Western blot analysis clarified its action mechanism associating with the activation of caspases and inhibition of PI3K/Akt pathway proteins. CONCLUSIONS: This is the first report regarding the apoptosis-inducing efficacy and mechanism of TCHD on SKM-1 cells, providing a promising candidate of TCM for MDS and AML therapy with fewer side effects.
- Date of acceptance
- 2016
- Autoren
- Jianping Jiang
- Bo Wang
- Jing Li
- Baodong Ye
- Shengyun Lin
- Wenbin Qian
- Letian Shan
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/27988397
- DOI
- 10.1016/j.jep.2016.12.012
- eISSN
- 1872-7573
- Zeitschrift
- J Ethnopharmacol
- Schlüsselwörter
- Apoptosis
- Hedyotis diffusa
- Myelodysplastic syndromes
- SKM-1
- Total coumarins
- Traditional Chinese medicine
- Apoptosis
- Caspases
- Cell Line
- Cell Proliferation
- Cells, Cultured
- Coumarins
- Hedyotis
- Humans
- Mesenchymal Stem Cells
- Myelodysplastic Syndromes
- Phosphatidylinositol 3-Kinases
- Proto-Oncogene Proteins c-akt
- Sprache
- eng
- Country
- Ireland
- Paginierung
- 253 - 260
- PII
- S0378-8741(16)32326-1
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2017
- Titel
- Total coumarins of Hedyotis diffusa induces apoptosis of myelodysplastic syndrome SKM-1 cells by activation of caspases and inhibition of PI3K/Akt pathway proteins.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 196
Data source: PubMed
- Beziehungen:
- Property of