Pharmacogenomic Characterization and Isobologram Analysis of the Combination of Ascorbic Acid and Curcumin-Two Main Metabolites of Curcuma longa-in cancer Cells
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Edna Ooko
- Onat Kadioglu
- Henry J Greten
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000393216000001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3389/fphar.2017.00038
- Externe Identifier
- Clarivate Analytics Document Solution ID: EJ4VU
- PubMed Identifier: 28210221
- ISSN
- 1663-9812
- Zeitschrift
- FRONTIERS IN PHARMACOLOGY
- Schlüsselwörter
- druginteraction
- isobologram analysis
- pharmacogenomics
- phytotherapy
- synergism
- Artikelnummer
- ARTN 38
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Titel
- Pharmacogenomic Characterization and Isobologram Analysis of the Combination of Ascorbic Acid and Curcumin-Two Main Metabolites of Curcuma longa-in cancer Cells
- Sub types
- Article
- Ausgabe der Zeitschrift
- 8
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Edna Ooko
- Onat Kadioglu
- Henry J Greten
- Thomas Efferth
- DOI
- 10.3389/fphar.2017.00038
- eISSN
- 1663-9812
- Zeitschrift
- Frontiers in Pharmacology
- Online publication date
- 2017
- Status
- Published online
- Herausgeber
- Frontiers Media SA
- Herausgeber URL
- http://dx.doi.org/10.3389/fphar.2017.00038
- Datum der Datenerfassung
- 2023
- Titel
- Pharmacogenomic Characterization and Isobologram Analysis of the Combination of Ascorbic Acid and Curcumin—Two Main Metabolites of Curcuma longa—in Cancer Cells
- Ausgabe der Zeitschrift
- 8
Data source: Crossref
- Abstract
- <i>Curcuma longa</i> has long been used in China and India as anti-inflammatory agent to treat a wide variety of conditions and also as a spice for varied curry preparations. The chemoprofile of the <i>Curcuma</i> species exhibits the presence of varied phytochemicals with curcumin being present in all three species but AA only being shown in <i>C. longa</i>. This study explored the effect of a curcumin/AA combination on human cancer cell lines. The curcumin/AA combination was assessed by isobologram analysis using the Loewe additivity drug interaction model. The drug combination showed additive cytotoxicity toward CCRF-CEM and CEM/ADR5000 leukemia cell lines and HCT116p53<sup>+/+</sup> and HCT116p53<sup>-/-</sup> colon cancer cell line, while the glioblastoma cell lines U87MG and U87MG.ΔEGFR showed additive to supra-additive cytotoxicity. Gene expression profiles predicting sensitivity and resistance of tumor cells to induction by curcumin and AA were determined by microarray-based mRNA expressions, COMPARE, and hierarchical cluster analyses. Numerous genes involved in transcription (<i>TFAM, TCERG1, RGS13, C11orf31</i>), apoptosis-regulation (<i>CRADD, CDK7, CDK19, CD81, TOM1</i>) signal transduction (<i>NR1D2, HMGN1, ABCA1, DE4ND4B, TRIM27</i>) DNA repair (<i>TOPBP1, RPA2</i>), mRNA metabolism (<i>RBBP4, HNRNPR, SRSF4, NR2F2, PDK1, TGM2</i>), and transporter genes (<i>ABCA1</i>) correlated with cellular responsiveness to curcumin and ascorbic acid. In conclusion, this study shows the effect of the curcumin/AA combination and identifies several candidate genes that may regulate the response of varied cancer cells to curcumin and AA.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University Mainz, Germany.
- Autoren
- Edna Ooko
- Onat Kadioglu
- Henry J Greten
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.3389/fphar.2017.00038
- eISSN
- 1663-9812
- Externe Identifier
- PubMed Identifier: 28210221
- PubMed Central ID: PMC5288649
- Open access
- true
- ISSN
- 1663-9812
- Zeitschrift
- Frontiers in pharmacology
- Sprache
- eng
- Medium
- Electronic-eCollection
- Online publication date
- 2017
- Open access status
- Open Access
- Paginierung
- 38
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2017
- Titel
- Pharmacogenomic Characterization and Isobologram Analysis of the Combination of Ascorbic Acid and Curcumin-Two Main Metabolites of <i>Curcuma longa</i>-in Cancer Cells.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 8
Files
https://www.frontiersin.org/articles/10.3389/fphar.2017.00038/pdf https://europepmc.org/articles/PMC5288649?pdf=render
Data source: Europe PubMed Central
- Abstract
- Curcuma longa has long been used in China and India as anti-inflammatory agent to treat a wide variety of conditions and also as a spice for varied curry preparations. The chemoprofile of the Curcuma species exhibits the presence of varied phytochemicals with curcumin being present in all three species but AA only being shown in C. longa. This study explored the effect of a curcumin/AA combination on human cancer cell lines. The curcumin/AA combination was assessed by isobologram analysis using the Loewe additivity drug interaction model. The drug combination showed additive cytotoxicity toward CCRF-CEM and CEM/ADR5000 leukemia cell lines and HCT116p53+/+ and HCT116p53-/- colon cancer cell line, while the glioblastoma cell lines U87MG and U87MG.ΔEGFR showed additive to supra-additive cytotoxicity. Gene expression profiles predicting sensitivity and resistance of tumor cells to induction by curcumin and AA were determined by microarray-based mRNA expressions, COMPARE, and hierarchical cluster analyses. Numerous genes involved in transcription (TFAM, TCERG1, RGS13, C11orf31), apoptosis-regulation (CRADD, CDK7, CDK19, CD81, TOM1) signal transduction (NR1D2, HMGN1, ABCA1, DE4ND4B, TRIM27) DNA repair (TOPBP1, RPA2), mRNA metabolism (RBBP4, HNRNPR, SRSF4, NR2F2, PDK1, TGM2), and transporter genes (ABCA1) correlated with cellular responsiveness to curcumin and ascorbic acid. In conclusion, this study shows the effect of the curcumin/AA combination and identifies several candidate genes that may regulate the response of varied cancer cells to curcumin and AA.
- Date of acceptance
- 2017
- Autoren
- Edna Ooko
- Onat Kadioglu
- Henry J Greten
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/28210221
- DOI
- 10.3389/fphar.2017.00038
- Externe Identifier
- PubMed Central ID: PMC5288649
- ISSN
- 1663-9812
- Zeitschrift
- Front Pharmacol
- Schlüsselwörter
- drug interaction
- isobologram analysis
- pharmacogenomics
- phytotherapy
- synergism
- Sprache
- eng
- Country
- Switzerland
- Paginierung
- 38
- Datum der Veröffentlichung
- 2017
- Status
- Published online
- Titel
- Pharmacogenomic Characterization and Isobologram Analysis of the Combination of Ascorbic Acid and Curcumin-Two Main Metabolites of Curcuma longa-in Cancer Cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 8
Data source: PubMed
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