Cytotoxicity and mode of action of a naturally occurring naphthoquinone, 2-acetyl-7-methoxynaphtho[2,3-b]furan-4,9-quinone towards multi-factorial drug-resistant cancer cells
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Victor Kuete
- Armelle T Mbaveng
- Louis P Sandjo
- Maen Zeino
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000410650900009&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.phymed.2017.07.010
- eISSN
- 1618-095X
- Externe Identifier
- Clarivate Analytics Document Solution ID: FG8BN
- PubMed Identifier: 28887921
- ISSN
- 0944-7113
- Zeitschrift
- PHYTOMEDICINE
- Schlüsselwörter
- 2-acetyl-7-methoxynaphtho[2,3-b]furan-4,9-quinone
- Cancer
- Cytotoxicity
- Milletia versicolor
- Multidrug-resistance
- Naphthoquinone
- Paginierung
- 62 - 68
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Titel
- Cytotoxicity and mode of action of a naturally occurring naphthoquinone, 2-acetyl-7-methoxynaphtho[2,3-b]furan-4,9-quinone towards multi-factorial drug-resistant cancer cells
- Sub types
- Article
- Ausgabe der Zeitschrift
- 33
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Victor Kuete
- Armelle T Mbaveng
- Louis P Sandjo
- Maen Zeino
- Thomas Efferth
- DOI
- 10.1016/j.phymed.2017.07.010
- ISSN
- 0944-7113
- Zeitschrift
- Phytomedicine
- Sprache
- en
- Paginierung
- 62 - 68
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.phymed.2017.07.010
- Datum der Datenerfassung
- 2023
- Titel
- Cytotoxicity and mode of action of a naturally occurring naphthoquinone, 2-acetyl-7-methoxynaphtho[2,3-b]furan-4,9-quinone towards multi-factorial drug-resistant cancer cells
- Ausgabe der Zeitschrift
- 33
Data source: Crossref
- Abstract
- <h4>Introduction</h4>Malignacies are still a major public concern worldwide and despite the intensive search of new chemotherapeutic agents, treatment still remains a challenging issue. The present study was designed to evaluate the cytotoxicity of 2-acetyl-7-methoxynaphtho[2,3-b]furan-4,9-quinone (AMNQ) isolated from the bark of Milletia versicolor towards a panel of drug-sensitive and multidrug-resistant (MDR) cancer cell lines.<h4>Methods</h4>The resazurin reduction assay was used to evaluate the cytotoxicity of AMNQ against 9 drug-sensitive and multidrug-resistant (MDR) cancer cell lines. Cell cycle, mitochondrial membrane potential (MMP) and levels of reactive oxygen species were all analyzed by flow cytometry.<h4>Results</h4>Following resazurin assay, the naphthoquinone AMNQ displayed IC<sub>50</sub> values ranging from 0.79 µM (against HepG2 hepatocarcinoma cells) to 3.26 µM (against MDA-MB231/BCRP breast cancer cells) on 9 tested cancer cell lines, whilst doxorubicin showed IC<sub>50</sub> values ranging from 0.40 µM (against CCRF-CEM leukemia cells) to 91.37 µM (against CEM/ADR5000 leukemia cells). IC<sub>50</sub> values below 1 µM were recorded with AMNQ towards CCRF-CEM cells (0.57 µM), U87MG.ΔEGFR gliobastoma multiforme cells (0.96 µM cells) and HepG2 cells (0.76 µM). Compared to its corresponding sensitive cell lines U87MG, sensitivity was observed in epidermal growth factor receptor-transfected U87MG.ΔEGFR cells to AMNQ. MMP was found to be the main mode of action of induction of apoptosis by AMNQ.<h4>Conclusions</h4>The results of this work demonstrate the cytotoxicity of AMNQ towards various types of cancer cell lines, including MDR phenotypes. AMNQ is a potential antiproliferative natural compound that deserves more investigations to develop novel cytotoxic drugs against sensitive and MDR cancers.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany; Department of Biochemistry, Faculty of Science, University of Dschang, Cameroon.
- Autoren
- Victor Kuete
- Armelle T Mbaveng
- Louis P Sandjo
- Maen Zeino
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1016/j.phymed.2017.07.010
- eISSN
- 1618-095X
- Externe Identifier
- PubMed Identifier: 28887921
- Open access
- false
- ISSN
- 0944-7113
- Zeitschrift
- Phytomedicine : international journal of phytotherapy and phytopharmacology
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Reactive Oxygen Species
- Naphthoquinones
- Doxorubicin
- Caspases
- Plant Extracts
- Antineoplastic Agents, Phytogenic
- Drug Resistance, Multiple
- Cell Cycle
- Apoptosis
- Drug Resistance, Neoplasm
- Membrane Potential, Mitochondrial
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2017
- Paginierung
- 62 - 68
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Datum der Datenerfassung
- 2017
- Titel
- Cytotoxicity and mode of action of a naturally occurring naphthoquinone, 2-acetyl-7-methoxynaphtho[2,3-b]furan-4,9-quinone towards multi-factorial drug-resistant cancer cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 33
Data source: Europe PubMed Central
- Abstract
- INTRODUCTION: Malignacies are still a major public concern worldwide and despite the intensive search of new chemotherapeutic agents, treatment still remains a challenging issue. The present study was designed to evaluate the cytotoxicity of 2-acetyl-7-methoxynaphtho[2,3-b]furan-4,9-quinone (AMNQ) isolated from the bark of Milletia versicolor towards a panel of drug-sensitive and multidrug-resistant (MDR) cancer cell lines. METHODS: The resazurin reduction assay was used to evaluate the cytotoxicity of AMNQ against 9 drug-sensitive and multidrug-resistant (MDR) cancer cell lines. Cell cycle, mitochondrial membrane potential (MMP) and levels of reactive oxygen species were all analyzed by flow cytometry. RESULTS: Following resazurin assay, the naphthoquinone AMNQ displayed IC50 values ranging from 0.79 µM (against HepG2 hepatocarcinoma cells) to 3.26 µM (against MDA-MB231/BCRP breast cancer cells) on 9 tested cancer cell lines, whilst doxorubicin showed IC50 values ranging from 0.40 µM (against CCRF-CEM leukemia cells) to 91.37 µM (against CEM/ADR5000 leukemia cells). IC50 values below 1 µM were recorded with AMNQ towards CCRF-CEM cells (0.57 µM), U87MG.ΔEGFR gliobastoma multiforme cells (0.96 µM cells) and HepG2 cells (0.76 µM). Compared to its corresponding sensitive cell lines U87MG, sensitivity was observed in epidermal growth factor receptor-transfected U87MG.ΔEGFR cells to AMNQ. MMP was found to be the main mode of action of induction of apoptosis by AMNQ. CONCLUSIONS: The results of this work demonstrate the cytotoxicity of AMNQ towards various types of cancer cell lines, including MDR phenotypes. AMNQ is a potential antiproliferative natural compound that deserves more investigations to develop novel cytotoxic drugs against sensitive and MDR cancers.
- Date of acceptance
- 2017
- Autoren
- Victor Kuete
- Armelle T Mbaveng
- Louis P Sandjo
- Maen Zeino
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/28887921
- DOI
- 10.1016/j.phymed.2017.07.010
- eISSN
- 1618-095X
- Zeitschrift
- Phytomedicine
- Schlüsselwörter
- 2-acetyl-7-methoxynaphtho[2,3-b]furan-4,9-quinone
- Cancer
- Cytotoxicity
- Milletia versicolor
- Multidrug-resistance
- Naphthoquinone
- Antineoplastic Agents, Phytogenic
- Apoptosis
- Caspases
- Cell Cycle
- Cell Line, Tumor
- Doxorubicin
- Drug Resistance, Multiple
- Drug Resistance, Neoplasm
- Humans
- Membrane Potential, Mitochondrial
- Naphthoquinones
- Plant Extracts
- Reactive Oxygen Species
- Sprache
- eng
- Country
- Germany
- Paginierung
- 62 - 68
- PII
- S0944-7113(17)30088-0
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2017
- Titel
- Cytotoxicity and mode of action of a naturally occurring naphthoquinone, 2-acetyl-7-methoxynaphtho[2,3-b]furan-4,9-quinone towards multi-factorial drug-resistant cancer cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 33
Data source: PubMed
- Beziehungen:
- Property of