Identification of Phlogacantholide C as a Novel ADAM10 Enhancer from Traditional Chinese Medicinal Plants
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Myriam Meineck
- Florian Schuck
- Sara Abdelfatah
- Thomas Efferth
- Kristina Endres
- DOI
- 10.3390/medicines3040030
- eISSN
- 2305-6320
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Medicines
- Sprache
- en
- Online publication date
- 2016
- Paginierung
- 30 - 30
- Status
- Published online
- Herausgeber
- MDPI AG
- Herausgeber URL
- http://dx.doi.org/10.3390/medicines3040030
- Datum der Datenerfassung
- 2019
- Titel
- Identification of Phlogacantholide C as a Novel ADAM10 Enhancer from Traditional Chinese Medicinal Plants
- Ausgabe der Zeitschrift
- 3
Data source: Crossref
- Other metadata sources:
-
- Abstract
- <b>Background:</b> Alzheimer's disease is one of the most prevalent dementias in the elderly population with increasing numbers of patients. One pivotal hallmark of this disorder is the deposition of protein aggregates stemming from neurotoxic amyloid-beta peptides. Synthesis of those peptides has been efficiently prevented in AD model mice by activation of an enzyme called alpha-secretase. Therefore, drugs with the capability to increase the expression of this enzyme, named ADAM10, have been suggested as a valuable therapeutic medication. <b>Methods:</b> We investigated 69 substances from a drug library derived from traditional Chinese medicine by luciferase reporter assay in human neuronal cells for their potential to selectively induce alpha-secretase expression. Western blot analysis was used to confirm results on the protein level. <b>Results:</b> Ten of the 69 investigated compounds led to induction of ADAM10 transcriptional activity while BACE-1 (beta-site APP cleaving enzyme 1) and APP (amyloid precursor protein) expression were not induced. Two of them-Norkurarinol and Phlogacantholide C-showed substantial elevation of ADAM10 protein levels and Phlogacantholide C also increased secretion of the ADAM10-derived cleavage product APPs-alpha. <b>Conclusion:</b> Phlogacantholide C represents a novel ADAM10 gene expression enhancer from traditional Chinese medicinal herbs that may lay the groundwork for evolving potential novel therapeutics in Alzheimer's disease.
- Addresses
- Clinic of Psychiatry and Psychotherapy, University Medical Center of the Johannes Gutenberg-University Mainz, 55131 Mainz, Germany. mmeineck@students.uni-mainz.de.
- Autoren
- Myriam Meineck
- Florian Schuck
- Sara Abdelfatah
- Thomas Efferth
- Kristina Endres
- DOI
- 10.3390/medicines3040030
- eISSN
- 2305-6320
- Externe Identifier
- PubMed Identifier: 28930140
- PubMed Central ID: PMC5456242
- Open access
- true
- ISSN
- 2305-6320
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Medicines (Basel, Switzerland)
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2016
- Open access status
- Open Access
- Paginierung
- E30
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2017
- Titel
- Identification of Phlogacantholide C as a Novel ADAM10 Enhancer from Traditional Chinese Medicinal Plants.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 3
Files
https://www.mdpi.com/2305-6320/3/4/30/pdf?version=1480932418 https://europepmc.org/articles/PMC5456242?pdf=render
Data source: Europe PubMed Central
- Abstract
- Background: Alzheimer's disease is one of the most prevalent dementias in the elderly population with increasing numbers of patients. One pivotal hallmark of this disorder is the deposition of protein aggregates stemming from neurotoxic amyloid-beta peptides. Synthesis of those peptides has been efficiently prevented in AD model mice by activation of an enzyme called alpha-secretase. Therefore, drugs with the capability to increase the expression of this enzyme, named ADAM10, have been suggested as a valuable therapeutic medication. Methods: We investigated 69 substances from a drug library derived from traditional Chinese medicine by luciferase reporter assay in human neuronal cells for their potential to selectively induce alpha-secretase expression. Western blot analysis was used to confirm results on the protein level. Results: Ten of the 69 investigated compounds led to induction of ADAM10 transcriptional activity while BACE-1 (beta-site APP cleaving enzyme 1) and APP (amyloid precursor protein) expression were not induced. Two of them-Norkurarinol and Phlogacantholide C-showed substantial elevation of ADAM10 protein levels and Phlogacantholide C also increased secretion of the ADAM10-derived cleavage product APPs-alpha. Conclusion: Phlogacantholide C represents a novel ADAM10 gene expression enhancer from traditional Chinese medicinal herbs that may lay the groundwork for evolving potential novel therapeutics in Alzheimer's disease.
- Date of acceptance
- 2016
- Autoren
- Myriam Meineck
- Florian Schuck
- Sara Abdelfatah
- Thomas Efferth
- Kristina Endres
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/28930140
- DOI
- 10.3390/medicines3040030
- Externe Identifier
- PubMed Central ID: PMC5456242
- ISSN
- 2305-6320
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Medicines (Basel)
- Schlüsselwörter
- ADAM10
- Alzheimer’s disease
- Amyloid precursor protein
- Norkurarinol
- Phlogacantholide C
- Phlogacanthus curviflorus
- Sophora flavescens
- Sprache
- eng
- Country
- Switzerland
- PII
- medicines3040030
- Datum der Veröffentlichung
- 2016
- Status
- Published online
- Titel
- Identification of Phlogacantholide C as a Novel ADAM10 Enhancer from Traditional Chinese Medicinal Plants.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 3
Data source: PubMed
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- Property of