In Vitro Study of the Cytotoxic, Cytostatic, and Antigenotoxic Profile of Hemidesmus indicus (L.) R.Br. (Apocynaceae) Crude Drug Extract on T Lymphoblastic Cells
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Eleonora Turrini
- Cinzia Calcabrini
- Massimo Tacchini
- Thomas Efferth
- Gianni Sacchetti
- Alessandra Guerrini
- Guglielmo Paganetto
- Elena Catanzaro
- Giulia Greco
- Carmela Fimognari
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000427543800022&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3390/toxins10020070
- Externe Identifier
- Clarivate Analytics Document Solution ID: FZ4EK
- PubMed Identifier: 29415441
- ISSN
- 2072-6651
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- TOXINS
- Schlüsselwörter
- Hemidesmus indicus
- cancer cells
- apoptosis
- cell cycle
- genotoxicity
- antigenotoxicity
- Artikelnummer
- ARTN 70
- Datum der Veröffentlichung
- 2018
- Status
- Published
- Titel
- In Vitro Study of the Cytotoxic, Cytostatic, and Antigenotoxic Profile of <i>Hemidesmus indicus</i> (L.) R.Br. (<i>Apocynaceae</i>) Crude Drug Extract on T Lymphoblastic Cells
- Sub types
- Article
- Ausgabe der Zeitschrift
- 10
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Eleonora Turrini
- Cinzia Calcabrini
- Massimo Tacchini
- Thomas Efferth
- Gianni Sacchetti
- Alessandra Guerrini
- Guglielmo Paganetto
- Elena Catanzaro
- Giulia Greco
- Carmela Fimognari
- DOI
- 10.3390/toxins10020070
- eISSN
- 2072-6651
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Toxins
- Sprache
- en
- Online publication date
- 2018
- Paginierung
- 70 - 70
- Status
- Published online
- Herausgeber
- MDPI AG
- Herausgeber URL
- http://dx.doi.org/10.3390/toxins10020070
- Datum der Datenerfassung
- 2018
- Titel
- In Vitro Study of the Cytotoxic, Cytostatic, and Antigenotoxic Profile of Hemidesmus indicus (L.) R.Br. (Apocynaceae) Crude Drug Extract on T Lymphoblastic Cells
- Ausgabe der Zeitschrift
- 10
Data source: Crossref
- Abstract
- In traditional Indian medicine, the crude drug <i>Hemidesmus indicus</i> root-commonly known as Indian sarsaparilla-is used alone or in poly-herbal preparations for the treatment of a wide range of diseases. The present study focuses on the cancer chemopreventive and therapeutic potential of <i>H. indicus</i> extracts on an acute lymphoblastic leukemia cell line (CCRF-CEM). With this aim in mind, we subjected <i>H. indicus</i> roots to two subsequent extractions (hydro-alcoholic extraction and soxhlet extraction). As DNA damage is an important prerequisite for the induction of mutations/cancer by genotoxic carcinogens, cancer chemoprevention may be achieved by preventing genotoxicity. Through an integrated experimental approach, we explored the genoprotective potential of the soxhlet <i>H. indicus</i> extract against different mutagenic compounds and its cytotoxic, proapoptotic, and cytostatic properties. In our experimental conditions, <i>H. indicus</i> induced a cytotoxic effect involving the activation of both intrinsic and extrinsic apoptotic pathways and blocked the cell cycle in the S phase. Moreover, the antigenotoxicity results showed that the extract was able to mitigate DNA damage, an essential mechanism for its applicability as a chemopreventive agent, via either the modulation of extracellular and intracellular events involved in DNA damage. These data add to the growing body of evidence that <i>H. indicus</i> can represent a noteworthy strategy to target early and late stages of cancer.
- Addresses
- Department for Life Quality Studies, Alma Mater Studiorum, University of Bologna, Corso d'Augusto 237, 47921 Rimini, Italy. eleonora.turrini@unibo.it.
- Autoren
- Eleonora Turrini
- Cinzia Calcabrini
- Massimo Tacchini
- Thomas Efferth
- Thomas Efferth
- Gianni Sacchetti
- Alessandra Guerrini
- Guglielmo Paganetto
- Elena Catanzaro
- Giulia Greco
- Carmela Fimognari
- DOI
- 10.3390/toxins10020070
- eISSN
- 2072-6651
- Externe Identifier
- PubMed Identifier: 29415441
- PubMed Central ID: PMC5848171
- Open access
- true
- ISSN
- 2072-6651
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Toxins
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Hemidesmus
- Plant Roots
- DNA Damage
- Plant Extracts
- Antineoplastic Agents, Phytogenic
- Protective Agents
- Cell Survival
- Precursor Cell Lymphoblastic Leukemia-Lymphoma
- Phytochemicals
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2018
- Open access status
- Open Access
- Paginierung
- E70
- Datum der Veröffentlichung
- 2018
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2018
- Titel
- In Vitro Study of the Cytotoxic, Cytostatic, and Antigenotoxic Profile of Hemidesmus indicus (L.) R.Br. (Apocynaceae) Crude Drug Extract on T Lymphoblastic Cells.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 10
Files
https://www.mdpi.com/2072-6651/10/2/70/pdf?version=1517903740 https://europepmc.org/articles/PMC5848171?pdf=render
Data source: Europe PubMed Central
- Abstract
- In traditional Indian medicine, the crude drug Hemidesmus indicus root-commonly known as Indian sarsaparilla-is used alone or in poly-herbal preparations for the treatment of a wide range of diseases. The present study focuses on the cancer chemopreventive and therapeutic potential of H. indicus extracts on an acute lymphoblastic leukemia cell line (CCRF-CEM). With this aim in mind, we subjected H. indicus roots to two subsequent extractions (hydro-alcoholic extraction and soxhlet extraction). As DNA damage is an important prerequisite for the induction of mutations/cancer by genotoxic carcinogens, cancer chemoprevention may be achieved by preventing genotoxicity. Through an integrated experimental approach, we explored the genoprotective potential of the soxhlet H. indicus extract against different mutagenic compounds and its cytotoxic, proapoptotic, and cytostatic properties. In our experimental conditions, H. indicus induced a cytotoxic effect involving the activation of both intrinsic and extrinsic apoptotic pathways and blocked the cell cycle in the S phase. Moreover, the antigenotoxicity results showed that the extract was able to mitigate DNA damage, an essential mechanism for its applicability as a chemopreventive agent, via either the modulation of extracellular and intracellular events involved in DNA damage. These data add to the growing body of evidence that H. indicus can represent a noteworthy strategy to target early and late stages of cancer.
- Date of acceptance
- 2018
- Autoren
- Eleonora Turrini
- Cinzia Calcabrini
- Massimo Tacchini
- Thomas Efferth
- Gianni Sacchetti
- Alessandra Guerrini
- Guglielmo Paganetto
- Elena Catanzaro
- Giulia Greco
- Carmela Fimognari
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/29415441
- DOI
- 10.3390/toxins10020070
- eISSN
- 2072-6651
- Externe Identifier
- PubMed Central ID: PMC5848171
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Toxins (Basel)
- Schlüsselwörter
- Hemidesmus indicus
- antigenotoxicity
- apoptosis
- cancer cells
- cell cycle
- genotoxicity
- Antineoplastic Agents, Phytogenic
- Cell Line, Tumor
- Cell Survival
- DNA Damage
- Hemidesmus
- Humans
- Phytochemicals
- Plant Extracts
- Plant Roots
- Precursor Cell Lymphoblastic Leukemia-Lymphoma
- Protective Agents
- Sprache
- eng
- Country
- Switzerland
- PII
- toxins10020070
- Datum der Veröffentlichung
- 2018
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2019
- Titel
- In Vitro Study of the Cytotoxic, Cytostatic, and Antigenotoxic Profile of Hemidesmus indicus (L.) R.Br. (Apocynaceae) Crude Drug Extract on T Lymphoblastic Cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 10
Data source: PubMed
- Beziehungen:
- Property of