Cytotoxicity of 18 Cameroonian medicinal plants against drug sensitive and multi-factorial drug resistant cancer cells

Publication type:
Journal article
Metadata:
Autoren
  • Armelle T Mbaveng
  • Hermione T Manekeng
  • Gaelle S Nguenang
  • Joachim K Dzotam
  • Victor Kuete
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000436224700003&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.jep.2018.04.036
Externe Identifier
  • Clarivate Analytics Document Solution ID: GK5OP
  • PubMed Identifier: 29709646
ISSN
0378-8741
Zeitschrift
JOURNAL OF ETHNOPHARMACOLOGY
Schlüsselwörter
  • Apoptosis
  • Cameroon
  • Curcuma longa cytotoxicity
  • Lycopersicon esculentum, Psidium guajava
Paginierung
21 - 33
Datum der Veröffentlichung
2018
Status
Published
Titel
Cytotoxicity of 18 Cameroonian medicinal plants against drug sensitive and multi-factorial drug resistant cancer cells
Sub types
  • Article
Ausgabe der Zeitschrift
222

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Armelle T Mbaveng
  • Hermione T Manekeng
  • Gaelle S Nguenang
  • Joachim K Dzotam
  • Victor Kuete
  • Thomas Efferth
DOI
10.1016/j.jep.2018.04.036
ISSN
0378-8741
Zeitschrift
Journal of Ethnopharmacology
Sprache
en
Paginierung
21 - 33
Datum der Veröffentlichung
2018
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.jep.2018.04.036
Datum der Datenerfassung
2023
Titel
Cytotoxicity of 18 Cameroonian medicinal plants against drug sensitive and multi-factorial drug resistant cancer cells
Ausgabe der Zeitschrift
222

Data source: Crossref

Abstract
<h4>Ethnopharmacological relevance</h4>Recommendations have been made stating that ethnopharmacological usages such as immune and skin disorders, inflammatory, infectious, parasitic and viral diseases should be taken into account if selecting plants for anticancer screening, since these reflect disease states bearing relevance to cancer or cancer-like symptoms. Cameroonian medicinal plants investigated in this work are traditionally used to treat cancer or ailments with relevance to cancer or cancer-like symptoms.<h4>Aim of the study</h4>In this study, 21 methanol extracts from 18 Cameroonian medicinal plants were tested in leukemia CCRF-CEM cells, and the best extracts were further tested on a panel of human cancer cell lines, including various multi-drug-resistant (MDR) phenotypes. Mechanistic studies were performed with the three best extracts.<h4>Materials and methods</h4>Resazurin reduction assay was used to evaluate cytotoxicity and ferroptotic effects of methanol extracts from different plants. Flow cytometry was used to analyze cell cycle, apoptosis, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS) of extracts from Curcuma longa rhizomes (CLR), Lycopersicon esculentum leaves (LEL), and Psidium guajava bark (PGB).<h4>Results</h4>In a pre-screening of all extracts, 13 out of 21 (61.9%) had IC<sub>50</sub> values below 80 µg/mL. Six of these active extracts displayed IC<sub>50</sub> values below 30 µg/mL: Cola pachycarpa leaves (CPL), Curcuma longa rhizomes (CLR), Lycopersicon esculentum leaves, Persea americana bark (PAB), Physalis peruviana twigs (PPT) and Psidium guajava bark (PGB). The best extracts displayed IC<sub>50</sub> values from 6.25 µg/mL (against HCT116 p53<sup>-/-</sup>) to 10.29 µg/mL (towards breast adenocarcinoma MDA-MB-231-BCRP cells) for CLR, from 9.64 µg/mL (against breast adenocarcinoma MDA-MB-231 cells) to 57.74 µg/mL (against HepG2 cells) for LEL and from 1.29 µg/mL (towards CEM/ADR5000 cells) to 62.64 µg/mL (towards MDA-MB-231 cells) for PGB. CLR and PGB induced apoptosis in CCRF-CEM cells via caspases activation, MMP depletion and increase ROS production whilst LEL induced apoptosis mediated by caspases activation and increase ROS production.<h4>Conclusion</h4>The best botanicals tested were CLR and LEL, which are worth to be explored in more detail to fight cancers including MDR phenotypes.
Addresses
  • Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany; Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon. Electronic address: armbatsa@yahoo.fr.
Autoren
  • Armelle T Mbaveng
  • Hermione T Manekeng
  • Gaelle S Nguenang
  • Joachim K Dzotam
  • Victor Kuete
  • Thomas Efferth
DOI
10.1016/j.jep.2018.04.036
eISSN
1872-7573
Externe Identifier
  • PubMed Identifier: 29709646
Funding acknowledgements
  • Alexander von Humboldt Foundation:
Open access
false
ISSN
0378-8741
Zeitschrift
Journal of ethnopharmacology
Schlüsselwörter
  • Cell Line, Tumor
  • Humans
  • Plants, Medicinal
  • Reactive Oxygen Species
  • Caspases
  • Plant Extracts
  • Antineoplastic Agents
  • Drug Resistance, Multiple
  • Apoptosis
  • Cell Survival
  • Drug Resistance, Neoplasm
  • Cameroon
  • Membrane Potential, Mitochondrial
  • Magnoliopsida
Sprache
eng
Medium
Print-Electronic
Online publication date
2018
Paginierung
21 - 33
Datum der Veröffentlichung
2018
Status
Published
Datum der Datenerfassung
2018
Titel
Cytotoxicity of 18 Cameroonian medicinal plants against drug sensitive and multi-factorial drug resistant cancer cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
222

Data source: Europe PubMed Central

Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Recommendations have been made stating that ethnopharmacological usages such as immune and skin disorders, inflammatory, infectious, parasitic and viral diseases should be taken into account if selecting plants for anticancer screening, since these reflect disease states bearing relevance to cancer or cancer-like symptoms. Cameroonian medicinal plants investigated in this work are traditionally used to treat cancer or ailments with relevance to cancer or cancer-like symptoms. AIM OF THE STUDY: In this study, 21 methanol extracts from 18 Cameroonian medicinal plants were tested in leukemia CCRF-CEM cells, and the best extracts were further tested on a panel of human cancer cell lines, including various multi-drug-resistant (MDR) phenotypes. Mechanistic studies were performed with the three best extracts. MATERIALS AND METHODS: Resazurin reduction assay was used to evaluate cytotoxicity and ferroptotic effects of methanol extracts from different plants. Flow cytometry was used to analyze cell cycle, apoptosis, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS) of extracts from Curcuma longa rhizomes (CLR), Lycopersicon esculentum leaves (LEL), and Psidium guajava bark (PGB). RESULTS: In a pre-screening of all extracts, 13 out of 21 (61.9%) had IC50 values below 80 µg/mL. Six of these active extracts displayed IC50 values below 30 µg/mL: Cola pachycarpa leaves (CPL), Curcuma longa rhizomes (CLR), Lycopersicon esculentum leaves, Persea americana bark (PAB), Physalis peruviana twigs (PPT) and Psidium guajava bark (PGB). The best extracts displayed IC50 values from 6.25 µg/mL (against HCT116 p53-/-) to 10.29 µg/mL (towards breast adenocarcinoma MDA-MB-231-BCRP cells) for CLR, from 9.64 µg/mL (against breast adenocarcinoma MDA-MB-231 cells) to 57.74 µg/mL (against HepG2 cells) for LEL and from 1.29 µg/mL (towards CEM/ADR5000 cells) to 62.64 µg/mL (towards MDA-MB-231 cells) for PGB. CLR and PGB induced apoptosis in CCRF-CEM cells via caspases activation, MMP depletion and increase ROS production whilst LEL induced apoptosis mediated by caspases activation and increase ROS production. CONCLUSION: The best botanicals tested were CLR and LEL, which are worth to be explored in more detail to fight cancers including MDR phenotypes.
Date of acceptance
2018
Autoren
  • Armelle T Mbaveng
  • Hermione T Manekeng
  • Gaelle S Nguenang
  • Joachim K Dzotam
  • Victor Kuete
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/29709646
DOI
10.1016/j.jep.2018.04.036
eISSN
1872-7573
Zeitschrift
J Ethnopharmacol
Schlüsselwörter
  • Apoptosis
  • Cameroon
  • Curcuma longa cytotoxicity
  • Deferoxamine
  • Doxorubicin
  • Ferrostatin-1
  • Geneticin
  • Hydrogen peroxide
  • Lycopersicon esculentum, Psidium guajava
  • Valinomycin
  • Antineoplastic Agents
  • Apoptosis
  • Cameroon
  • Caspases
  • Cell Line, Tumor
  • Cell Survival
  • Drug Resistance, Multiple
  • Drug Resistance, Neoplasm
  • Humans
  • Magnoliopsida
  • Membrane Potential, Mitochondrial
  • Plant Extracts
  • Plants, Medicinal
  • Reactive Oxygen Species
Sprache
eng
Country
Ireland
Paginierung
21 - 33
PII
S0378-8741(17)34321-0
Datum der Veröffentlichung
2018
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2018
Titel
Cytotoxicity of 18 Cameroonian medicinal plants against drug sensitive and multi-factorial drug resistant cancer cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
222

Data source: PubMed

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