Cytotoxicity of epunctanone and four other phytochemicals isolated from the medicinal plants Garcinia epunctata and Ptycholobium contortum towards multi-factorial drug resistant cancer cells

Publication type:
Journal article
Metadata:
Autoren
  • Armelle T Mbaveng
  • Ghislain W Fotso
  • Dominique Ngnintedo
  • Victor Kuete
  • Bonaventure T Ngadjui
  • Felix Keumedjio
  • Kerstin Andrae-Marobela
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000443714600013&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.phymed.2017.12.016
Externe Identifier
  • Clarivate Analytics Document Solution ID: GS5OG
  • PubMed Identifier: 30195869
ISSN
0944-7113
Zeitschrift
PHYTOMEDICINE
Schlüsselwörter
  • Apoptosis
  • Benzophenone
  • Cytotoxicity
  • Multi-drug resistance
  • Ferroptosis
  • Phytochemicals
Paginierung
112 - 119
Datum der Veröffentlichung
2018
Status
Published
Titel
Cytotoxicity of epunctanone and four other phytochemicals isolated from the medicinal plants <i>Garcinia epunctata</i> and <i>Ptycholobium contortum</i> towards multi-factorial drug resistant cancer cells
Sub types
  • Article
Ausgabe der Zeitschrift
48

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Armelle T Mbaveng
  • Ghislain W Fotso
  • Dominique Ngnintedo
  • Victor Kuete
  • Bonaventure T Ngadjui
  • Felix Keumedjio
  • Kerstin Andrae-Marobela
  • Thomas Efferth
DOI
10.1016/j.phymed.2017.12.016
ISSN
0944-7113
Zeitschrift
Phytomedicine
Sprache
en
Paginierung
112 - 119
Datum der Veröffentlichung
2018
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.phymed.2017.12.016
Datum der Datenerfassung
2023
Titel
Cytotoxicity of epunctanone and four other phytochemicals isolated from the medicinal plants Garcinia epunctata and Ptycholobium contortum towards multi-factorial drug resistant cancer cells
Ausgabe der Zeitschrift
48

Data source: Crossref

Abstract
<h4>Introduction</h4>Resistance of cancer cells is a serious impediment to chemotherapy and several phytochemicals are active against multi-drug resistant (MDR) phenotypes. The cytotoxicity of five naturally occurring compounds: betulin (1), mundulea lactone (2), seputhecarpan A (3), seputheisoflavone (4) and epunctanone (5) was evaluated on a panel of 9 cancer cell lines including various sensitive and drug-resistant cell lines. The modes of action of compound 5 were further investigated.<h4>Methods</h4>The resazurin reduction assay was used to evaluate cytotoxicity of samples and ferroptotic cell death induced by compound 5; caspase-Glo assay was used to detect the activation of caspases in CCRF-CEM leukemia cells treated with compound 5. Flow cytometry was used for cell cycle analysis in CCRF-CEM cells treated with compound 5, as well as detection of apoptotic cells by annexin V/PI staining, analysis of mitochondrial membrane potential (MMP) and measurement of reactive oxygen species (ROS).<h4>Results</h4>Compounds 1-5 displayed cytotoxic effects in the 9 studied cancer cell lines with IC<sub>50</sub> values below 70 µM. The IC<sub>50</sub> values varied from 8.20 µM (in HCT116 (p53<sup>-/-</sup>) colon cancer cells) to 35.10 µM (against HepG2 hepatocarcinoma cells) for 1, from 8.84 µM (in CEM/ADR5000 leukemia cells) to 48.99 µM (in MDA-MB-231 breast adenocarcinoma cells) for 2, from 12.17 µM (in CEM/ADR5000 cells) to 65.08 µM (in MDA-MB-231 cells) for 3, from 23.80 µM (in U87MG.ΔEGFR glioblastoma cells) to 68.66 µM (in HCT116 (p53<sup>-/-</sup>) cells) for 4, from 4.84 µM (in HCT116 (p53<sup>-/-</sup>) cells) to 13.12 µM (in HepG2 cells) for 5 and from 0.02 µM (against CCRF-CEM cells) to 122.96 µM (in CEM/ADR5000 cells) for doxorubicin. Compound 5 induced apoptosis in CCRF-CEM cells through alteration of MMP and increase in ROS production. In addition to apoptosis, ferroptosis was also identified as another mode of cell death induced by epunctanone.<h4>Conclusions</h4>Compounds 1-5 are valuable cytotoxic compounds that could be used to combat MDR cancer cells. Benzophenoe 5 is the most active molecule and deserve more investigations to develop new anticancer drugs.
Addresses
  • Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany; Department of Biochemistry, Faculty of Science, University of Dschang, Cameroon.
Autoren
  • Armelle T Mbaveng
  • Ghislain W Fotso
  • Dominique Ngnintedo
  • Victor Kuete
  • Bonaventure T Ngadjui
  • Felix Keumedjio
  • Kerstin Andrae-Marobela
  • Thomas Efferth
DOI
10.1016/j.phymed.2017.12.016
eISSN
1618-095X
Externe Identifier
  • PubMed Identifier: 30195869
Open access
false
ISSN
0944-7113
Zeitschrift
Phytomedicine : international journal of phytotherapy and phytopharmacology
Schlüsselwörter
  • Cell Line, Tumor
  • Humans
  • Fabaceae
  • Garcinia
  • Plants, Medicinal
  • Reactive Oxygen Species
  • Doxorubicin
  • Caspases
  • Plant Extracts
  • Antineoplastic Agents, Phytogenic
  • Drug Resistance, Multiple
  • Apoptosis
  • Molecular Structure
  • Drug Resistance, Neoplasm
  • Membrane Potential, Mitochondrial
  • Hep G2 Cells
  • Phytochemicals
Sprache
eng
Medium
Print-Electronic
Online publication date
2017
Paginierung
112 - 119
Datum der Veröffentlichung
2018
Status
Published
Datum der Datenerfassung
2018
Titel
Cytotoxicity of epunctanone and four other phytochemicals isolated from the medicinal plants Garcinia epunctata and Ptycholobium contortum towards multi-factorial drug resistant cancer cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
48

Data source: Europe PubMed Central

Abstract
INTRODUCTION: Resistance of cancer cells is a serious impediment to chemotherapy and several phytochemicals are active against multi-drug resistant (MDR) phenotypes. The cytotoxicity of five naturally occurring compounds: betulin (1), mundulea lactone (2), seputhecarpan A (3), seputheisoflavone (4) and epunctanone (5) was evaluated on a panel of 9 cancer cell lines including various sensitive and drug-resistant cell lines. The modes of action of compound 5 were further investigated. METHODS: The resazurin reduction assay was used to evaluate cytotoxicity of samples and ferroptotic cell death induced by compound 5; caspase-Glo assay was used to detect the activation of caspases in CCRF-CEM leukemia cells treated with compound 5. Flow cytometry was used for cell cycle analysis in CCRF-CEM cells treated with compound 5, as well as detection of apoptotic cells by annexin V/PI staining, analysis of mitochondrial membrane potential (MMP) and measurement of reactive oxygen species (ROS). RESULTS: Compounds 1-5 displayed cytotoxic effects in the 9 studied cancer cell lines with IC50 values below 70 µM. The IC50 values varied from 8.20 µM (in HCT116 (p53-/-) colon cancer cells) to 35.10 µM (against HepG2 hepatocarcinoma cells) for 1, from 8.84 µM (in CEM/ADR5000 leukemia cells) to 48.99 µM (in MDA-MB-231 breast adenocarcinoma cells) for 2, from 12.17 µM (in CEM/ADR5000 cells) to 65.08 µM (in MDA-MB-231 cells) for 3, from 23.80 µM (in U87MG.ΔEGFR glioblastoma cells) to 68.66 µM (in HCT116 (p53-/-) cells) for 4, from 4.84 µM (in HCT116 (p53-/-) cells) to 13.12 µM (in HepG2 cells) for 5 and from 0.02 µM (against CCRF-CEM cells) to 122.96 µM (in CEM/ADR5000 cells) for doxorubicin. Compound 5 induced apoptosis in CCRF-CEM cells through alteration of MMP and increase in ROS production. In addition to apoptosis, ferroptosis was also identified as another mode of cell death induced by epunctanone. CONCLUSIONS: Compounds 1-5 are valuable cytotoxic compounds that could be used to combat MDR cancer cells. Benzophenoe 5 is the most active molecule and deserve more investigations to develop new anticancer drugs.
Date of acceptance
2017
Autoren
  • Armelle T Mbaveng
  • Ghislain W Fotso
  • Dominique Ngnintedo
  • Victor Kuete
  • Bonaventure T Ngadjui
  • Felix Keumedjio
  • Kerstin Andrae-Marobela
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/30195869
DOI
10.1016/j.phymed.2017.12.016
eISSN
1618-095X
Zeitschrift
Phytomedicine
Schlüsselwörter
  • Apoptosis
  • Benzophenone
  • Cytotoxicity
  • Ferroptosis
  • Multi-drug resistance
  • Phytochemicals
  • Antineoplastic Agents, Phytogenic
  • Apoptosis
  • Caspases
  • Cell Line, Tumor
  • Doxorubicin
  • Drug Resistance, Multiple
  • Drug Resistance, Neoplasm
  • Fabaceae
  • Garcinia
  • Hep G2 Cells
  • Humans
  • Membrane Potential, Mitochondrial
  • Molecular Structure
  • Phytochemicals
  • Plant Extracts
  • Plants, Medicinal
  • Reactive Oxygen Species
Sprache
eng
Country
Germany
Paginierung
112 - 119
PII
S0944-7113(17)30190-3
Datum der Veröffentlichung
2018
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2018
Titel
Cytotoxicity of epunctanone and four other phytochemicals isolated from the medicinal plants Garcinia epunctata and Ptycholobium contortum towards multi-factorial drug resistant cancer cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
48

Data source: PubMed

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