Intra-Articular Injection of Fructus Ligustri Lucidi Extract Attenuates Pain Behavior and Cartilage Degeneration in Mono-Iodoacetate Induced Osteoarthritic Rats
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Bo Yan
- Li Zhou
- Caiwei Wang
- Rongrong Wang
- Li Yan
- Lingying Yu
- Fucun Liu
- Wenxi Du
- Guangping Yu
- Qiang Yuan
- Peijian Tong
- Letian Shan
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000451094300001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3389/fphar.2018.01360
- Externe Identifier
- Clarivate Analytics Document Solution ID: HB5IT
- PubMed Identifier: 30532708
- ISSN
- 1663-9812
- Zeitschrift
- FRONTIERS IN PHARMACOLOGY
- Schlüsselwörter
- Fructus Ligustri Lucidi
- intra-articular
- chondrocyte
- hypertrophy
- mono-iodoacetate
- Artikelnummer
- ARTN 1360
- Datum der Veröffentlichung
- 2018
- Status
- Published
- Titel
- Intra-Articular Injection of <i>Fructus Ligustri Lucidi</i> Extract Attenuates Pain Behavior and Cartilage Degeneration in Mono-Iodoacetate Induced Osteoarthritic Rats
- Sub types
- Article
- Ausgabe der Zeitschrift
- 9
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Bo Yan
- Li Zhou
- Caiwei Wang
- Rongrong Wang
- Li Yan
- Lingying Yu
- Fucun Liu
- Wenxi Du
- Guangping Yu
- Qiang Yuan
- Peijian Tong
- Letian Shan
- Thomas Efferth
- DOI
- 10.3389/fphar.2018.01360
- eISSN
- 1663-9812
- Zeitschrift
- Frontiers in Pharmacology
- Online publication date
- 2018
- Status
- Published online
- Herausgeber
- Frontiers Media SA
- Herausgeber URL
- http://dx.doi.org/10.3389/fphar.2018.01360
- Datum der Datenerfassung
- 2019
- Titel
- Intra-Articular Injection of Fructus Ligustri Lucidi Extract Attenuates Pain Behavior and Cartilage Degeneration in Mono-Iodoacetate Induced Osteoarthritic Rats
- Ausgabe der Zeitschrift
- 9
Data source: Crossref
- Abstract
- <i>Fructus Ligustri Lucidi</i> (FLL) has been widely used as a traditional Chinese medicine (TCM) for treating soreness and weakness of waist and knees. It has potential for treating OA owing to its kidney-tonifying activity with bone-strengthening effects, but there is so far no report of its anti-OA effect. This study established a rat OA model by intra-articular (IA) injection of mono-iodoacetate (1.5 mg) and weekly treated by IA administration of FLL at 100 μg/mL for 4 weeks. Thermal withdrawal latency, mechanical withdrawal threshold, and spontaneous activity were tested for evaluation of pain behavior, and histopathological (HE, SO, and ABH staining) and immunohistochemical (Col2, Col10, and MMP13) analyses were conducted for observation of cartilage degradation. <i>In vitro</i> effect of FLL on chondrocytes was evaluated by MTT assay and qPCR analysis. Moreover, HPLC analysis was performed to determine its chemoprofile. The pain behavioral data showed that FLL attenuated joint pain hypersensitivity by increasing thresholds of mechanical allodynia and thermal hyperalgesia as well as spontaneous activity. The histopathological result showed that FLL reversed OA cartilage degradation by protecting chondrocytes and extracellular matrix in cartilage, and the immunohistochemical analysis revealed its molecular actions on protein expressions of MMP13, Col2, and Col10 in cartilage. The MTT assay showed its proliferative effects on chondrocytes, and qPCR assay clarified its mechanism associated with gene expressions of <i>Mmp13</i>, <i>Col2</i>, <i>Col10</i>, <i>Adamts5</i>, <i>Aggrecan</i>, and <i>Runx2</i> in TNF-α treated chondrocytes. Our results revealed an anti-OA effect of FLL on pain behavior and cartilage degradation in OA rats and clarified a molecular mechanism in association with the suppression of chondrocyte hypertrophy and catabolism. IA FLL can be regarded as novel and promising option for OA therapy.
- Addresses
- The First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, China.
- Autoren
- Bo Yan
- Li Zhou
- Caiwei Wang
- Rongrong Wang
- Li Yan
- Lingying Yu
- Fucun Liu
- Wenxi Du
- Guangping Yu
- Qiang Yuan
- Peijian Tong
- Letian Shan
- Thomas Efferth
- DOI
- 10.3389/fphar.2018.01360
- eISSN
- 1663-9812
- Externe Identifier
- PubMed Identifier: 30532708
- PubMed Central ID: PMC6265644
- Open access
- true
- ISSN
- 1663-9812
- Zeitschrift
- Frontiers in pharmacology
- Sprache
- eng
- Medium
- Electronic-eCollection
- Online publication date
- 2018
- Open access status
- Open Access
- Paginierung
- 1360
- Datum der Veröffentlichung
- 2018
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2018
- Titel
- Intra-Articular Injection of <i>Fructus Ligustri Lucidi</i> Extract Attenuates Pain Behavior and Cartilage Degeneration in Mono-Iodoacetate Induced Osteoarthritic Rats.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 9
Files
https://www.frontiersin.org/articles/10.3389/fphar.2018.01360/pdf https://europepmc.org/articles/PMC6265644?pdf=render
Data source: Europe PubMed Central
- Abstract
- Fructus Ligustri Lucidi (FLL) has been widely used as a traditional Chinese medicine (TCM) for treating soreness and weakness of waist and knees. It has potential for treating OA owing to its kidney-tonifying activity with bone-strengthening effects, but there is so far no report of its anti-OA effect. This study established a rat OA model by intra-articular (IA) injection of mono-iodoacetate (1.5 mg) and weekly treated by IA administration of FLL at 100 μg/mL for 4 weeks. Thermal withdrawal latency, mechanical withdrawal threshold, and spontaneous activity were tested for evaluation of pain behavior, and histopathological (HE, SO, and ABH staining) and immunohistochemical (Col2, Col10, and MMP13) analyses were conducted for observation of cartilage degradation. In vitro effect of FLL on chondrocytes was evaluated by MTT assay and qPCR analysis. Moreover, HPLC analysis was performed to determine its chemoprofile. The pain behavioral data showed that FLL attenuated joint pain hypersensitivity by increasing thresholds of mechanical allodynia and thermal hyperalgesia as well as spontaneous activity. The histopathological result showed that FLL reversed OA cartilage degradation by protecting chondrocytes and extracellular matrix in cartilage, and the immunohistochemical analysis revealed its molecular actions on protein expressions of MMP13, Col2, and Col10 in cartilage. The MTT assay showed its proliferative effects on chondrocytes, and qPCR assay clarified its mechanism associated with gene expressions of Mmp13, Col2, Col10, Adamts5, Aggrecan, and Runx2 in TNF-α treated chondrocytes. Our results revealed an anti-OA effect of FLL on pain behavior and cartilage degradation in OA rats and clarified a molecular mechanism in association with the suppression of chondrocyte hypertrophy and catabolism. IA FLL can be regarded as novel and promising option for OA therapy.
- Date of acceptance
- 2018
- Autoren
- Bo Yan
- Li Zhou
- Caiwei Wang
- Rongrong Wang
- Li Yan
- Lingying Yu
- Fucun Liu
- Wenxi Du
- Guangping Yu
- Qiang Yuan
- Peijian Tong
- Letian Shan
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/30532708
- DOI
- 10.3389/fphar.2018.01360
- Externe Identifier
- PubMed Central ID: PMC6265644
- ISSN
- 1663-9812
- Zeitschrift
- Front Pharmacol
- Schlüsselwörter
- Fructus Ligustri Lucidi
- chondrocyte
- hypertrophy
- intra-articular
- mono-iodoacetate
- Sprache
- eng
- Country
- Switzerland
- Paginierung
- 1360
- Datum der Veröffentlichung
- 2018
- Status
- Published online
- Titel
- Intra-Articular Injection of Fructus Ligustri Lucidi Extract Attenuates Pain Behavior and Cartilage Degeneration in Mono-Iodoacetate Induced Osteoarthritic Rats.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 9
Data source: PubMed
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