Effects of anti-inflammatory and adaptogenic herbal extracts on gene expression of eicosanoids signaling pathways in isolated brain cells

Publication type:
Journal article
Metadata:
Autoren
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000485781500003&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.phymed.2019.152881
eISSN
1618-095X
Externe Identifier
  • Clarivate Analytics Document Solution ID: IX6GQ
  • PubMed Identifier: 30987861
ISSN
0944-7113
Zeitschrift
PHYTOMEDICINE
Schlüsselwörter
  • Adaptogen
  • Eicosanoids
  • Inflammaging RNA sequencing
  • Pathway analysis
  • Alzheimer disease
Artikelnummer
ARTN 152881
Datum der Veröffentlichung
2019
Status
Published
Titel
Effects of anti-inflammatory and adaptogenic herbal extracts on gene expression of eicosanoids signaling pathways in isolated brain cells
Sub types
  • Article
Ausgabe der Zeitschrift
60

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
DOI
10.1016/j.phymed.2019.152881
ISSN
0944-7113
Zeitschrift
Phytomedicine
Sprache
en
Artikelnummer
152881
Paginierung
152881 - 152881
Datum der Veröffentlichung
2019
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.phymed.2019.152881
Datum der Datenerfassung
2023
Titel
Effects of anti-inflammatory and adaptogenic herbal extracts on gene expression of eicosanoids signaling pathways in isolated brain cells
Ausgabe der Zeitschrift
60

Data source: Crossref

Abstract
<h4>Introduction</h4>The adaptogens modulate expression of genes playing key roles in development of aging-related disorders, which are considered as low-grade systemic inflammatory conditions characterized by an imbalance between pro-and anti-inflammatory eicosanoids.<h4>Aim of the study</h4>We compared the effects of anti-inflammatory and adaptogenic plant extracts on the expression of genes involved in biosynthesis of eicosanoids with the purpose to find those plants, which selectively upregulated the expression of anti-inflammatory lipoxins signaling pathways and inhibited pro-inflammatory signaling pathways associated with biosynthesis of leukotrienes, prostaglandins and thromboxanes.<h4>Materials and methods</h4>We conducted transcriptome-wide RNA sequencing to profile gene expression alterations in T98G neuroglia cells upon treatment of plant extract and analyzed the relevance of deregulated genes to eicosanoids signaling pathways using in silico models.<h4>Results</h4>For the first time, we demonstrated that Rhodiola rosea, Withania somnifera and Eleutherococcus senticosus downregulate the expression of key genes (ALOX5AP, DPEP2, LTC4S) involved biosynthesis of leukotrienes A, B, C, D and E, resulting in inhibition of leukotriene signaling pathway suggesting their potential benefits in Alzheimer disease. The common feature for all tested anti-inflammatory plants extracts was related to downregulation of ALOX12, which was also associated with neuroprotective action of these medicinal plants as well as their potential benefits in neurodegenerative diseases. None of tested anti-inflammatory and adaptogenic plants selectively activated the ALOX15-mediated signaling pathway, which is associated with generation anti-inflammatory lipoxins. Almost all tested plants upregulated the expression of the prostaglandin E receptor 3 gene (PTGER3) suggesting their potential benefits in the treatment of cancer.<h4>Conclusion</h4>Every single plant tested in this study revealed a specific "signature" on eicosanoid signaling-related gene expression, regardless of their common features as anti-inflammatory or adaptogenic activity. Further studies of the combination of Rhodiola with Withania (Adaptra) for the treatment of Alzheimer disease are required.
Addresses
  • EuroPharma USA Inc., 955 Challenger Dr., Green Bay, WI 54311 USA; Phytomed AB, Vaxtorp, Sweden. Electronic address: apanossian@europharmausa.com.
Autoren
DOI
10.1016/j.phymed.2019.152881
eISSN
1618-095X
Externe Identifier
  • PubMed Identifier: 30987861
Funding acknowledgements
  • Terrence Lemerond: 2014-01
  • EuroPharma USA Inc.:
Open access
false
ISSN
0944-7113
Zeitschrift
Phytomedicine : international journal of phytotherapy and phytopharmacology
Schlüsselwörter
  • Neuroglia
  • Humans
  • Eleutherococcus
  • Rhodiola
  • Withania
  • Plants, Medicinal
  • Alzheimer Disease
  • Eicosanoids
  • Leukotrienes
  • Anti-Inflammatory Agents
  • Plant Extracts
  • Sequence Analysis, RNA
  • Signal Transduction
  • Gene Expression Regulation
  • Transcriptome
Sprache
eng
Medium
Print-Electronic
Online publication date
2019
Paginierung
152881
Datum der Veröffentlichung
2019
Status
Published
Publisher licence
CC BY-NC-ND
Datum der Datenerfassung
2019
Titel
Effects of anti-inflammatory and adaptogenic herbal extracts on gene expression of eicosanoids signaling pathways in isolated brain cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
60

Data source: Europe PubMed Central

Abstract
INTRODUCTION: The adaptogens modulate expression of genes playing key roles in development of aging-related disorders, which are considered as low-grade systemic inflammatory conditions characterized by an imbalance between pro-and anti-inflammatory eicosanoids. AIM OF THE STUDY: We compared the effects of anti-inflammatory and adaptogenic plant extracts on the expression of genes involved in biosynthesis of eicosanoids with the purpose to find those plants, which selectively upregulated the expression of anti-inflammatory lipoxins signaling pathways and inhibited pro-inflammatory signaling pathways associated with biosynthesis of leukotrienes, prostaglandins and thromboxanes. MATERIALS AND METHODS: We conducted transcriptome-wide RNA sequencing to profile gene expression alterations in T98G neuroglia cells upon treatment of plant extract and analyzed the relevance of deregulated genes to eicosanoids signaling pathways using in silico models. RESULTS: For the first time, we demonstrated that Rhodiola rosea, Withania somnifera and Eleutherococcus senticosus downregulate the expression of key genes (ALOX5AP, DPEP2, LTC4S) involved biosynthesis of leukotrienes A, B, C, D and E, resulting in inhibition of leukotriene signaling pathway suggesting their potential benefits in Alzheimer disease. The common feature for all tested anti-inflammatory plants extracts was related to downregulation of ALOX12, which was also associated with neuroprotective action of these medicinal plants as well as their potential benefits in neurodegenerative diseases. None of tested anti-inflammatory and adaptogenic plants selectively activated the ALOX15-mediated signaling pathway, which is associated with generation anti-inflammatory lipoxins. Almost all tested plants upregulated the expression of the prostaglandin E receptor 3 gene (PTGER3) suggesting their potential benefits in the treatment of cancer. CONCLUSION: Every single plant tested in this study revealed a specific "signature" on eicosanoid signaling-related gene expression, regardless of their common features as anti-inflammatory or adaptogenic activity. Further studies of the combination of Rhodiola with Withania (Adaptra) for the treatment of Alzheimer disease are required.
Date of acceptance
2019
Autoren
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/30987861
DOI
10.1016/j.phymed.2019.152881
eISSN
1618-095X
Zeitschrift
Phytomedicine
Schlüsselwörter
  • Adaptogen
  • Alzheimer disease
  • Eicosanoids
  • Inflammaging RNA sequencing
  • Pathway analysis
  • Alzheimer Disease
  • Anti-Inflammatory Agents
  • Eicosanoids
  • Eleutherococcus
  • Gene Expression Regulation
  • Humans
  • Leukotrienes
  • Neuroglia
  • Plant Extracts
  • Plants, Medicinal
  • Rhodiola
  • Sequence Analysis, RNA
  • Signal Transduction
  • Transcriptome
  • Withania
Sprache
eng
Country
Germany
Paginierung
152881
PII
S0944-7113(19)30051-0
Datum der Veröffentlichung
2019
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2019
Titel
Effects of anti-inflammatory and adaptogenic herbal extracts on gene expression of eicosanoids signaling pathways in isolated brain cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
60

Data source: PubMed

Beziehungen:
Property of

Tools