Cytotoxicity of 40 Egyptian plant extracts targeting mechanisms of drug-resistant cancer cells
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Mohamed-Elamir F Hegazy
- Sara Abdelfatan
- Ahmed R Hamed
- Tarik A Mohamed
- Abdelsamed A Elshamy
- Ibrahim A Saleh
- Eman H Reda
- Nahla S Abdel-Azim
- Khaled A Shams
- Mahmoud Sakr
- Yoshikazu Sugimoto
- Paul W Pare
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000473048600008&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.phymed.2018.11.031
- eISSN
- 1618-095X
- Externe Identifier
- Clarivate Analytics Document Solution ID: IF4JW
- PubMed Identifier: 31055230
- ISSN
- 0944-7113
- Zeitschrift
- PHYTOMEDICINE
- Schlüsselwörter
- Cytotoxicity
- Mode of action
- Multidrug resistance
- Wild medicinal plants
- Egypt
- Artikelnummer
- ARTN 152771
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Titel
- Cytotoxicity of 40 Egyptian plant extracts targeting mechanisms of drug-resistant cancer cells
- Sub types
- Article
- Ausgabe der Zeitschrift
- 59
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Mohamed-Elamir F Hegazy
- Sara Abdelfatah
- Ahmed R Hamed
- Tarik A Mohamed
- Abdelsamed A Elshamy
- Ibrahim A Saleh
- Eman H Reda
- Nahla S Abdel-Azim
- Khaled A Shams
- Mahmoud Sakr
- Yoshikazu Sugimoto
- Paul W Paré
- Thomas Efferth
- DOI
- 10.1016/j.phymed.2018.11.031
- ISSN
- 0944-7113
- Zeitschrift
- Phytomedicine
- Sprache
- en
- Artikelnummer
- 152771
- Paginierung
- 152771 - 152771
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.phymed.2018.11.031
- Datum der Datenerfassung
- 2019
- Titel
- Cytotoxicity of 40 Egyptian plant extracts targeting mechanisms of drug-resistant cancer cells
- Ausgabe der Zeitschrift
- 59
Data source: Crossref
- Abstract
- <h4>Background</h4>The multidrug resistance (MDR) phenotype encounters a major challenge to the success of established chemotherapy in cancer patients. We hypothesized that cytotoxic medicinal plants with novel phytochemicals can overcome MDR and kill MDR-cells with similar efficacy as drug sensitive cells.<h4>Purpose</h4>We evaluated plant extracts from an unexplored ecosystem in Egypt with unusual climate and nutrient conditions for their activity against sensitive and multidrug-resistant cancer cell lines.<h4>Material and methods/study design</h4>Methylene chloride: methanol (1:1) and methanol: H<sub>2</sub>O (7:3) extracts of 40 plants were prepared resulting in a sum of 76 fraction containing compounds with varying polarity. The resazurin reduction assay was employed to evaluate the cytotoxicity of these extracts on five matched pairs of drug-sensitive and their drug-resistant cell lines. Flow cytometry and Western blotting was used to determine cell cycle analyses, apoptosis, and autophagy. Reactive oxygen species (ROS) were measured spectrophotometrically.<h4>Results</h4>Extracts derived from Withania obtusifolia (WO), Jasonia candicans (JC), Centaurea lippii (CL), and Pulicaria undulata (PU) were the most active ones among 76 extracts from 40 Egyptian medicinal plants. They showed a significant reduction of cell viability on drug-sensitive CCRF-CEM leukemia cell line with IC<sub>50</sub> values less than 7 µg/ml. Low cross-resistance degrees were observed in multidrug-resistant CEM/ADR5000 cells towards CL (1.82-fold) and JC (6.09-fold). All other drug-resistant cell lines did not reveal cross-resistance to the four extracts. Further mechanistic assessment have been studied for these four extracts.<h4>Conclusion</h4>The methylene chloride: methanol (1:1) fractions of WO, JC, CL, and PU are promising cytotoxic extracts that could be used to combat MDR cancer cells through different cell death pathways.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Staudinger Weg 5, Mainz 55128, Germany; Chemistry of Medicinal Plants Department, National Research Centre, 33 El-Bohouth St., Dokki, Giza 12622, Egypt.
- Autoren
- Mohamed-Elamir F Hegazy
- Sara Abdelfatah
- Ahmed R Hamed
- Tarik A Mohamed
- Abdelsamed A Elshamy
- Ibrahim A Saleh
- Eman H Reda
- Nahla S Abdel-Azim
- Khaled A Shams
- Mahmoud Sakr
- Yoshikazu Sugimoto
- Paul W Paré
- Thomas Efferth
- DOI
- 10.1016/j.phymed.2018.11.031
- eISSN
- 1618-095X
- Externe Identifier
- PubMed Identifier: 31055230
- Open access
- false
- ISSN
- 0944-7113
- Zeitschrift
- Phytomedicine : international journal of phytotherapy and phytopharmacology
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Centaurea
- Withania
- Plants, Medicinal
- Neoplasms
- Reactive Oxygen Species
- Plant Extracts
- Antineoplastic Agents, Phytogenic
- Phytotherapy
- Drug Resistance, Multiple
- Apoptosis
- Cell Survival
- Drug Resistance, Neoplasm
- Egypt
- Membrane Potential, Mitochondrial
- Pulicaria
- Phytochemicals
- Magnoliopsida
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2018
- Paginierung
- 152771
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Datum der Datenerfassung
- 2019
- Titel
- Cytotoxicity of 40 Egyptian plant extracts targeting mechanisms of drug-resistant cancer cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 59
Data source: Europe PubMed Central
- Abstract
- BACKGROUND: The multidrug resistance (MDR) phenotype encounters a major challenge to the success of established chemotherapy in cancer patients. We hypothesized that cytotoxic medicinal plants with novel phytochemicals can overcome MDR and kill MDR-cells with similar efficacy as drug sensitive cells. PURPOSE: We evaluated plant extracts from an unexplored ecosystem in Egypt with unusual climate and nutrient conditions for their activity against sensitive and multidrug-resistant cancer cell lines. MATERIAL AND METHODS/STUDY DESIGN: Methylene chloride: methanol (1:1) and methanol: H2O (7:3) extracts of 40 plants were prepared resulting in a sum of 76 fraction containing compounds with varying polarity. The resazurin reduction assay was employed to evaluate the cytotoxicity of these extracts on five matched pairs of drug-sensitive and their drug-resistant cell lines. Flow cytometry and Western blotting was used to determine cell cycle analyses, apoptosis, and autophagy. Reactive oxygen species (ROS) were measured spectrophotometrically. RESULTS: Extracts derived from Withania obtusifolia (WO), Jasonia candicans (JC), Centaurea lippii (CL), and Pulicaria undulata (PU) were the most active ones among 76 extracts from 40 Egyptian medicinal plants. They showed a significant reduction of cell viability on drug-sensitive CCRF-CEM leukemia cell line with IC50 values less than 7 µg/ml. Low cross-resistance degrees were observed in multidrug-resistant CEM/ADR5000 cells towards CL (1.82-fold) and JC (6.09-fold). All other drug-resistant cell lines did not reveal cross-resistance to the four extracts. Further mechanistic assessment have been studied for these four extracts. CONCLUSION: The methylene chloride: methanol (1:1) fractions of WO, JC, CL, and PU are promising cytotoxic extracts that could be used to combat MDR cancer cells through different cell death pathways.
- Date of acceptance
- 2018
- Autoren
- Mohamed-Elamir F Hegazy
- Sara Abdelfatah
- Ahmed R Hamed
- Tarik A Mohamed
- Abdelsamed A Elshamy
- Ibrahim A Saleh
- Eman H Reda
- Nahla S Abdel-Azim
- Khaled A Shams
- Mahmoud Sakr
- Yoshikazu Sugimoto
- Paul W Paré
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/31055230
- DOI
- 10.1016/j.phymed.2018.11.031
- eISSN
- 1618-095X
- Zeitschrift
- Phytomedicine
- Schlüsselwörter
- Cytotoxicity
- Egypt
- Mode of action
- Multidrug resistance
- Wild medicinal plants
- Antineoplastic Agents, Phytogenic
- Apoptosis
- Cell Line, Tumor
- Cell Survival
- Centaurea
- Drug Resistance, Multiple
- Drug Resistance, Neoplasm
- Egypt
- Humans
- Magnoliopsida
- Membrane Potential, Mitochondrial
- Neoplasms
- Phytochemicals
- Phytotherapy
- Plant Extracts
- Plants, Medicinal
- Pulicaria
- Reactive Oxygen Species
- Withania
- Sprache
- eng
- Country
- Germany
- Paginierung
- 152771
- PII
- S0944-7113(18)30588-9
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2019
- Titel
- Cytotoxicity of 40 Egyptian plant extracts targeting mechanisms of drug-resistant cancer cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 59
Data source: PubMed
- Beziehungen:
- Property of