Antiulcer activity of Cyperus alternifolius in relation to its UPLC-MS metabolite fingerprint: A mechanistic study

Publication type:
Journal article
Metadata:
Autoren
  • Abdel Razik H Farrag
  • Heba MI Abdallah
  • Amira R Khattab
  • Abdelsamed I Elshamy
  • Abd El-Nasser G El Gendy
  • Tarik A Mohamed
  • Mohamed A Farag
  • Thomas Efferth
  • Mohamed-Elamir F Hegazy
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000500559500032&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.phymed.2019.152970
eISSN
1618-095X
Externe Identifier
  • Clarivate Analytics Document Solution ID: JS8NV
  • PubMed Identifier: 31181403
ISSN
0944-7113
Zeitschrift
PHYTOMEDICINE
Schlüsselwörter
  • Anti-inflammatory
  • Cyperus alternifolius
  • Cyperaceae
  • Gastric ulcer
  • Metabolomics
  • UPLC-MS
Artikelnummer
ARTN 152970
Datum der Veröffentlichung
2019
Status
Published
Titel
Antiulcer activity of <i>Cyperus alternifolius</i> in relation to its UPLC-MS metabolite fingerprint: A mechanistic study
Sub types
  • Article
Ausgabe der Zeitschrift
62

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Abdel Razik H Farrag
  • Heba MI Abdallah
  • Amira R Khattab
  • Abdelsamed I Elshamy
  • Abd El-Nasser G El Gendy
  • Tarik A Mohamed
  • Mohamed A Farag
  • Thomas Efferth
  • Mohamed-Elamir F Hegazy
DOI
10.1016/j.phymed.2019.152970
ISSN
0944-7113
Zeitschrift
Phytomedicine
Sprache
en
Artikelnummer
152970
Paginierung
152970 - 152970
Datum der Veröffentlichung
2019
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.phymed.2019.152970
Datum der Datenerfassung
2022
Titel
Antiulcer activity of Cyperus alternifolius in relation to its UPLC-MS metabolite fingerprint: A mechanistic study
Ausgabe der Zeitschrift
62

Data source: Crossref

Abstract
<h4>Background</h4>Gastric ulcer is one of the main prevalent gastrointestinal multi-etiological disorders with many associated complications and adverse effects. Our aim was to develop safer antiulcer therapies based on methanol or ethyl acetate extracts of tubers and aerial parts from Cyperus alternifolius.<h4>Methods</h4>Gastric ulceration was experimentally generated by administration of single oral doses of indomethacin (30 mg/kg) to fasted rats. The animals received methanol or ethyl acetate extracts of C. alternifolius tuber and methanol or ethyl acetate extracts of aerial parts at two dose levels (50 or 100 mg/kg). Ranitidine (50 mg/kg) was used as standard anti-ulcer drug. After 4 h, the ulcer number and the total ulcer score were determined and TNF-α was assessed. Also, pathological and histochemical examination for gastric mucosa were performed. The metabolome heterogeneity of the different extracts was explored using (UPLC-MS) aided by supervised pattern recognition, i.e., orthogonal partial least squares discriminate analysis (OPLS-DA). A second OPLS-DA model was employed to link the UPLC-MS derived metabolome of the different extracts to their antiulcer activity to identify activity mediating metabolites.<h4>Results</h4>The extracts significantly reduced ulcer number, total ulcer score and TNF-α content in the stomach. Methanol or ethyl acetate extracts of tubers were most effective even more than ranitidine. In parallel, the histopathological examination showed an improvement of damaged mucosa. A high PAS reaction was observed in the treated groups indicating a relieve of the mucosal layer. A mechanistic clue of the C. alternifolius antiulcer potential was provided by the identification of its bioactive compounds using OPLS-DA. Both methanol extracts of tubers and aerial parts were more enriched in phenolic acids. The ethyl acetate extract of the aerial part was more abundant in two aldehydes. A mechanism of action was postulated based on their reported actions viz. α-carbonic anhydrase inhibition, anti-inflammatory and analgesic activity by its antioxidant activity and downregulation of several inflammatory mediators.<h4>Conclusion</h4>This is the first study to report on the antiulcer activity of C. alternifolius tubers with identification of the key bioactive compounds and the mode of action. Future phytochemical and biological evaluation of the identified bioactive compounds are needed to confirm the plant tubers as safer alternative or adjunct therapy compared to conventional antiulcer drugs.
Addresses
  • Pathology Department; National Research Centre, Dokki, Giza 12622, Egypt.
Autoren
  • Abdel Razik H Farrag
  • Heba MI Abdallah
  • Amira R Khattab
  • Abdelsamed I Elshamy
  • Abd El-Nasser G El Gendy
  • Tarik A Mohamed
  • Mohamed A Farag
  • Thomas Efferth
  • Mohamed-Elamir F Hegazy
DOI
10.1016/j.phymed.2019.152970
eISSN
1618-095X
Externe Identifier
  • PubMed Identifier: 31181403
Funding acknowledgements
  • American University in Cairo:
  • American University: 1 – 18)−2017-Nov-10-16-26-34
  • Takeda Science Foundation:
  • Alexander von Humboldt-Stiftung:
Open access
false
ISSN
0944-7113
Zeitschrift
Phytomedicine : international journal of phytotherapy and phytopharmacology
Schlüsselwörter
  • Gastric Mucosa
  • Animals
  • Rats
  • Rats, Wistar
  • Cyperus
  • Plant Components, Aerial
  • Plant Tubers
  • Stomach Ulcer
  • Methanol
  • Acetic Acid
  • Acetates
  • Indomethacin
  • Anti-Inflammatory Agents
  • Anti-Ulcer Agents
  • Plant Extracts
  • Chromatography, Liquid
  • Male
  • Tandem Mass Spectrometry
  • Metabolomics
  • Metabolome
  • Phytochemicals
Sprache
eng
Medium
Print-Electronic
Online publication date
2019
Paginierung
152970
Datum der Veröffentlichung
2019
Status
Published
Datum der Datenerfassung
2019
Titel
Antiulcer activity of Cyperus alternifolius in relation to its UPLC-MS metabolite fingerprint: A mechanistic study.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
62

Data source: Europe PubMed Central

Abstract
BACKGROUND: Gastric ulcer is one of the main prevalent gastrointestinal multi-etiological disorders with many associated complications and adverse effects. Our aim was to develop safer antiulcer therapies based on methanol or ethyl acetate extracts of tubers and aerial parts from Cyperus alternifolius. METHODS: Gastric ulceration was experimentally generated by administration of single oral doses of indomethacin (30 mg/kg) to fasted rats. The animals received methanol or ethyl acetate extracts of C. alternifolius tuber and methanol or ethyl acetate extracts of aerial parts at two dose levels (50 or 100 mg/kg). Ranitidine (50 mg/kg) was used as standard anti-ulcer drug. After 4 h, the ulcer number and the total ulcer score were determined and TNF-α was assessed. Also, pathological and histochemical examination for gastric mucosa were performed. The metabolome heterogeneity of the different extracts was explored using (UPLC-MS) aided by supervised pattern recognition, i.e., orthogonal partial least squares discriminate analysis (OPLS-DA). A second OPLS-DA model was employed to link the UPLC-MS derived metabolome of the different extracts to their antiulcer activity to identify activity mediating metabolites. RESULTS: The extracts significantly reduced ulcer number, total ulcer score and TNF-α content in the stomach. Methanol or ethyl acetate extracts of tubers were most effective even more than ranitidine. In parallel, the histopathological examination showed an improvement of damaged mucosa. A high PAS reaction was observed in the treated groups indicating a relieve of the mucosal layer. A mechanistic clue of the C. alternifolius antiulcer potential was provided by the identification of its bioactive compounds using OPLS-DA. Both methanol extracts of tubers and aerial parts were more enriched in phenolic acids. The ethyl acetate extract of the aerial part was more abundant in two aldehydes. A mechanism of action was postulated based on their reported actions viz. α-carbonic anhydrase inhibition, anti-inflammatory and analgesic activity by its antioxidant activity and downregulation of several inflammatory mediators. CONCLUSION: This is the first study to report on the antiulcer activity of C. alternifolius tubers with identification of the key bioactive compounds and the mode of action. Future phytochemical and biological evaluation of the identified bioactive compounds are needed to confirm the plant tubers as safer alternative or adjunct therapy compared to conventional antiulcer drugs.
Date of acceptance
2019
Autoren
  • Abdel Razik H Farrag
  • Heba MI Abdallah
  • Amira R Khattab
  • Abdelsamed I Elshamy
  • Abd El-Nasser G El Gendy
  • Tarik A Mohamed
  • Mohamed A Farag
  • Thomas Efferth
  • Mohamed-Elamir F Hegazy
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/31181403
DOI
10.1016/j.phymed.2019.152970
eISSN
1618-095X
Zeitschrift
Phytomedicine
Schlüsselwörter
  • Anti-inflammatory
  • Cyperaceae
  • Cyperus alternifolius
  • Gastric ulcer
  • Metabolomics
  • UPLC-MS
  • Acetates
  • Acetic Acid
  • Animals
  • Anti-Inflammatory Agents
  • Anti-Ulcer Agents
  • Chromatography, Liquid
  • Cyperus
  • Gastric Mucosa
  • Indomethacin
  • Male
  • Metabolome
  • Metabolomics
  • Methanol
  • Phytochemicals
  • Plant Components, Aerial
  • Plant Extracts
  • Plant Tubers
  • Rats
  • Rats, Wistar
  • Stomach Ulcer
  • Tandem Mass Spectrometry
Sprache
eng
Country
Germany
Paginierung
152970
PII
S0944-7113(19)30138-2
Datum der Veröffentlichung
2019
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2020
Titel
Antiulcer activity of Cyperus alternifolius in relation to its UPLC-MS metabolite fingerprint: A mechanistic study.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
62

Data source: PubMed

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