Pinolenic acid ameliorates oleic acid-induced lipogenesis and oxidative stress via AMPK/SIRT1 signaling pathway in HepG2 cells

Publication type:
Journal article
Metadata:
Autoren
  • Jing Zhang
  • Sun-dong Zhang
  • Peng Wang
  • Na Guo
  • Wei Wang
  • Li-ping Yao
  • Qing Yang
  • Thomas Efferth
  • Jiao Jiao
  • Yu-jie Fu
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000483936600018&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.ejphar.2019.172618
eISSN
1879-0712
Externe Identifier
  • Clarivate Analytics Document Solution ID: IU9YM
  • PubMed Identifier: 31430456
ISSN
0014-2999
Zeitschrift
EUROPEAN JOURNAL OF PHARMACOLOGY
Schlüsselwörter
  • Pinolenic acid
  • Lipogenesis
  • NAFLD
  • AMPK
  • Oxidative stress
Artikelnummer
ARTN 172618
Datum der Veröffentlichung
2019
Status
Published
Titel
Pinolenic acid ameliorates oleic acid-induced lipogenesis and oxidative stress <i>via</i> AMPK/SIRT1 signaling pathway in HepG2 cells
Sub types
  • Article
Ausgabe der Zeitschrift
861

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Jing Zhang
  • Sun-dong Zhang
  • Peng Wang
  • Na Guo
  • Wei Wang
  • Li-ping Yao
  • Qing Yang
  • Thomas Efferth
  • Jiao Jiao
  • Yu-jie Fu
DOI
10.1016/j.ejphar.2019.172618
ISSN
0014-2999
Zeitschrift
European Journal of Pharmacology
Sprache
en
Artikelnummer
172618
Paginierung
172618 - 172618
Datum der Veröffentlichung
2019
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.ejphar.2019.172618
Datum der Datenerfassung
2022
Titel
Pinolenic acid ameliorates oleic acid-induced lipogenesis and oxidative stress via AMPK/SIRT1 signaling pathway in HepG2 cells
Ausgabe der Zeitschrift
861

Data source: Crossref

Abstract
Pinolenic acid (PLA), a natural compound isolated from pine nut oil, has been reported to exert bioactivity against lipid anabolism. Nonetheless, the underlying mechanisms still poorly elucidated. The aim of this study is to comprehensively demonstrate the effects of PLA on oleic acid (OA)-induced non-alcoholic fatty liver disease (NAFLD) and their relationship with the lipid metabolic regulation. The results demonstrated that treatment with PLA dramatically inhibited lipid accumulation, oxidative stress as well as inflammatory responses induced by oleic acid in HepG2 cells. PLA also obviously decreased the levels of cellular triglyceride (TG), total cholesterol (TC), malondialdehyde (MDA), reactive oxygen species (ROS) and nitric oxide (NO). As well as PLA stilled promoted the antioxidant enzymes activity including superoxide dismutase (SOD) and glutathione peroxidase (GPX). Furthermore, PLA could increase the expressions of nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase1 (HO-1) to alleviate oxidative damage. It also could reduce lipogenesis-related transcription factors expression, such as sterol regulatory element-binding protein 1 (SREBP1c), fatty acid synthase (FASN) and stearoyl-CoA desaturase 1 (SCD1). PLA treatment resulted in increasing phosphorylation of AMP-activated protein kinase (AMPK) and peroxisome proliferator-activated receptor-α (PPARα) expression. However, pretreatment with compound C (inhibitor of AMPK) inhibited the effect of PLA on promoting the expression of p-AMPK, SIRT1 and PPARα for lipolysis. Taken together, these results demonstrated that PLA possessed the potential to prevent lipid accumulation in OA-induced HepG2 cells via upregulating the AMPK/SIRT1 signaling pathway, which supported the development of new drug candidate against non-alcoholic steatohepatitis.
Addresses
  • Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, 150040, China.
Autoren
  • Jing Zhang
  • Sun-Dong Zhang
  • Peng Wang
  • Na Guo
  • Wei Wang
  • Li-Ping Yao
  • Qing Yang
  • Thomas Efferth
  • Jiao Jiao
  • Yu-Jie Fu
DOI
10.1016/j.ejphar.2019.172618
eISSN
1879-0712
Externe Identifier
  • PubMed Identifier: 31430456
Funding acknowledgements
  • National Key Research and Development Program of China: 2016YFD0600805
  • Fundamental Research Funds for the Central Universities: 2572017AA10
  • Harbin Medical University:
  • Fundamental Research Funds for the Central Universities: 2572017ET03
  • Ministry of Education of the People&amp;apos;s Republic of China: KF201620
  • Key Laboratory of Myocardial Ischemia, Ministry of Education:
  • Fundamental Research Funds for the Central Universities: 2572017EA03
Open access
false
ISSN
0014-2999
Zeitschrift
European journal of pharmacology
Schlüsselwörter
  • Humans
  • Nitric Oxide
  • Linolenic Acids
  • Oleic Acid
  • PPAR alpha
  • Signal Transduction
  • Gene Expression Regulation
  • Oxidative Stress
  • Lipogenesis
  • AMP-Activated Protein Kinases
  • Sirtuin 1
  • Hep G2 Cells
Sprache
eng
Medium
Print-Electronic
Online publication date
2019
Paginierung
172618
Datum der Veröffentlichung
2019
Status
Published
Datum der Datenerfassung
2019
Titel
Pinolenic acid ameliorates oleic acid-induced lipogenesis and oxidative stress via AMPK/SIRT1 signaling pathway in HepG2 cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
861

Data source: Europe PubMed Central

Abstract
Pinolenic acid (PLA), a natural compound isolated from pine nut oil, has been reported to exert bioactivity against lipid anabolism. Nonetheless, the underlying mechanisms still poorly elucidated. The aim of this study is to comprehensively demonstrate the effects of PLA on oleic acid (OA)-induced non-alcoholic fatty liver disease (NAFLD) and their relationship with the lipid metabolic regulation. The results demonstrated that treatment with PLA dramatically inhibited lipid accumulation, oxidative stress as well as inflammatory responses induced by oleic acid in HepG2 cells. PLA also obviously decreased the levels of cellular triglyceride (TG), total cholesterol (TC), malondialdehyde (MDA), reactive oxygen species (ROS) and nitric oxide (NO). As well as PLA stilled promoted the antioxidant enzymes activity including superoxide dismutase (SOD) and glutathione peroxidase (GPX). Furthermore, PLA could increase the expressions of nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase1 (HO-1) to alleviate oxidative damage. It also could reduce lipogenesis-related transcription factors expression, such as sterol regulatory element-binding protein 1 (SREBP1c), fatty acid synthase (FASN) and stearoyl-CoA desaturase 1 (SCD1). PLA treatment resulted in increasing phosphorylation of AMP-activated protein kinase (AMPK) and peroxisome proliferator-activated receptor-α (PPARα) expression. However, pretreatment with compound C (inhibitor of AMPK) inhibited the effect of PLA on promoting the expression of p-AMPK, SIRT1 and PPARα for lipolysis. Taken together, these results demonstrated that PLA possessed the potential to prevent lipid accumulation in OA-induced HepG2 cells via upregulating the AMPK/SIRT1 signaling pathway, which supported the development of new drug candidate against non-alcoholic steatohepatitis.
Date of acceptance
2019
Autoren
  • Jing Zhang
  • Sun-Dong Zhang
  • Peng Wang
  • Na Guo
  • Wei Wang
  • Li-Ping Yao
  • Qing Yang
  • Thomas Efferth
  • Jiao Jiao
  • Yu-Jie Fu
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/31430456
DOI
10.1016/j.ejphar.2019.172618
eISSN
1879-0712
Zeitschrift
Eur J Pharmacol
Schlüsselwörter
  • AMPK
  • Lipogenesis
  • NAFLD
  • Oxidative stress
  • Pinolenic acid
  • AMP-Activated Protein Kinases
  • Gene Expression Regulation
  • Hep G2 Cells
  • Humans
  • Linolenic Acids
  • Lipogenesis
  • Nitric Oxide
  • Oleic Acid
  • Oxidative Stress
  • PPAR alpha
  • Signal Transduction
  • Sirtuin 1
Sprache
eng
Country
Netherlands
Paginierung
172618
PII
S0014-2999(19)30570-9
Datum der Veröffentlichung
2019
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2020
Titel
Pinolenic acid ameliorates oleic acid-induced lipogenesis and oxidative stress via AMPK/SIRT1 signaling pathway in HepG2 cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
861

Data source: PubMed

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