8,8-bis-(Dihydroconiferyl)-diferulate displayed impressive cytotoxicity towards a panel of human and animal cancer cells
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Armelle T Mbaveng
- Francois Damen
- Michel-Gael F Guefack
- Simplice Beaudelaire Tankeo
- Sara Abdelfatah
- Gabin TM Bitchagno
- Ilhami Celik
- Victor Kuete
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000532046800002&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.phymed.2020.153215
- eISSN
- 1618-095X
- Externe Identifier
- Clarivate Analytics Document Solution ID: LM1XQ
- PubMed Identifier: 32388040
- ISSN
- 0944-7113
- Zeitschrift
- PHYTOMEDICINE
- Schlüsselwörter
- 8, 8-bis-(dihydroconiferyl)-diferulate
- Diferulic acid
- Cell death
- Multi-drug resistance
- Natural product
- Artikelnummer
- ARTN 153215
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Titel
- 8,8-bis-(Dihydroconiferyl)-diferulate displayed impressive cytotoxicity towards a panel of human and animal cancer cells
- Sub types
- Article
- Ausgabe der Zeitschrift
- 70
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Armelle T Mbaveng
- Francois Damen
- Michel-Gael F Guefack
- Simplice Beaudelaire Tankeo
- Sara Abdelfatah
- Gabin TM Bitchagno
- İlhami Çelik
- Victor Kuete
- Thomas Efferth
- DOI
- 10.1016/j.phymed.2020.153215
- ISSN
- 0944-7113
- Zeitschrift
- Phytomedicine
- Sprache
- en
- Artikelnummer
- 153215
- Paginierung
- 153215 - 153215
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.phymed.2020.153215
- Datum der Datenerfassung
- 2023
- Titel
- 8,8-bis-(Dihydroconiferyl)-diferulate displayed impressive cytotoxicity towards a panel of human and animal cancer cells
- Ausgabe der Zeitschrift
- 70
Data source: Crossref
- Abstract
- <h4>Background</h4>Recalcitrant cancers appear as a major obstacle to chemotherapy, prompting scientists to intensify the search for novel drugs to tackle the cell lines expressing multi-drug resistant (MDR) phenotypes.<h4>Purpose</h4>The purpose of this study was to evaluate the antiproliferative potential of a ferrulic acid derivative, 8,8-bis-(dihydroconiferyl)-diferulate (DHCF2) on a panel of 18 cancer cell lines, including various sensitive and drug-resistant phenotypes, belonging to human and animals. The mode of induction of cell death by this compound was further studied.<h4>Methods</h4>The antiproliferative activity, autophagy, ferroptotic and necroptotic cell death were evaluated by the resazurin reduction assay (RRA). CCRF-CEM leukemia cells were used for all mechanistic studies. A caspase-Glo assay was applied to evaluate the activity of caspases. Cell cycle analysis (PI staining), apoptosis (annexin V/PI staining), mitochondrial membrane potential (MMP) (JC-1) and reactive oxygen species (ROS) (H<sub>2</sub>DCFH-DA) were assessed by flow cytometry.<h4>Results</h4>DHCF2 demonstrated impressive cytotoxic effects towards the 18 cancer cell lines tested, with IC<sub>50</sub> values all below 6.5 µM. The obtained IC<sub>50</sub> values were in the range of 1.17 µM (towards CCRF-CEM leukemia cells) to 6.34 µM (towards drug-resistant HCT116 p53<sup>-/-</sup> human colon adenocarcinoma cells) for DHCF2 and from 0.02 µM (against CCRF-CEM cells) to 122.96 µM (against multidrug-resistant CEM/ADR5000 leukemia cells) for the reference drug, doxorubicin. DHCF2 had IC<sub>50</sub> values lower than those of doxorubicin, against CEM/ADR5000 cells and on some melanoma cell lines, such as MaMel-80a cells, Mel-2a cells, MV3 cells and SKMel-505 cells. DHCF2 induced autophagy as well as apoptosis in CCRF-CEM cells though caspases activation, MMP alteration and increase of ROS production.<h4>Conclusion</h4>The studied diferulic acid, DHCF2, is a promising antiproliferative compound. It deserves further indepth investigations with the ultimate aim to develop a novel drug to fight cancer drug resistance.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany; Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
- Autoren
- Armelle T Mbaveng
- Francois Damen
- Michel-Gael F Guefack
- Simplice Beaudelaire Tankeo
- Sara Abdelfatah
- Gabin TM Bitchagno
- İlhami Çelik
- Victor Kuete
- Thomas Efferth
- DOI
- 10.1016/j.phymed.2020.153215
- eISSN
- 1618-095X
- Externe Identifier
- PubMed Identifier: 32388040
- Open access
- false
- ISSN
- 0944-7113
- Zeitschrift
- Phytomedicine : international journal of phytotherapy and phytopharmacology
- Schlüsselwörter
- Cell Line, Tumor
- Animals
- Humans
- Mice
- Leukemia
- Doxorubicin
- Plant Extracts
- Antineoplastic Agents, Phytogenic
- Drug Resistance, Multiple
- Apoptosis
- Drug Resistance, Neoplasm
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2020
- Paginierung
- 153215
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Datum der Datenerfassung
- 2020
- Titel
- 8,8-bis-(Dihydroconiferyl)-diferulate displayed impressive cytotoxicity towards a panel of human and animal cancer cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 70
Data source: Europe PubMed Central
- Abstract
- BACKGROUND: Recalcitrant cancers appear as a major obstacle to chemotherapy, prompting scientists to intensify the search for novel drugs to tackle the cell lines expressing multi-drug resistant (MDR) phenotypes. PURPOSE: The purpose of this study was to evaluate the antiproliferative potential of a ferrulic acid derivative, 8,8-bis-(dihydroconiferyl)-diferulate (DHCF2) on a panel of 18 cancer cell lines, including various sensitive and drug-resistant phenotypes, belonging to human and animals. The mode of induction of cell death by this compound was further studied. METHODS: The antiproliferative activity, autophagy, ferroptotic and necroptotic cell death were evaluated by the resazurin reduction assay (RRA). CCRF-CEM leukemia cells were used for all mechanistic studies. A caspase-Glo assay was applied to evaluate the activity of caspases. Cell cycle analysis (PI staining), apoptosis (annexin V/PI staining), mitochondrial membrane potential (MMP) (JC-1) and reactive oxygen species (ROS) (H2DCFH-DA) were assessed by flow cytometry. RESULTS: DHCF2 demonstrated impressive cytotoxic effects towards the 18 cancer cell lines tested, with IC50 values all below 6.5 µM. The obtained IC50 values were in the range of 1.17 µM (towards CCRF-CEM leukemia cells) to 6.34 µM (towards drug-resistant HCT116 p53-/- human colon adenocarcinoma cells) for DHCF2 and from 0.02 µM (against CCRF-CEM cells) to 122.96 µM (against multidrug-resistant CEM/ADR5000 leukemia cells) for the reference drug, doxorubicin. DHCF2 had IC50 values lower than those of doxorubicin, against CEM/ADR5000 cells and on some melanoma cell lines, such as MaMel-80a cells, Mel-2a cells, MV3 cells and SKMel-505 cells. DHCF2 induced autophagy as well as apoptosis in CCRF-CEM cells though caspases activation, MMP alteration and increase of ROS production. CONCLUSION: The studied diferulic acid, DHCF2, is a promising antiproliferative compound. It deserves further indepth investigations with the ultimate aim to develop a novel drug to fight cancer drug resistance.
- Date of acceptance
- 2020
- Autoren
- Armelle T Mbaveng
- Francois Damen
- Michel-Gael F Guefack
- Simplice Beaudelaire Tankeo
- Sara Abdelfatah
- Gabin TM Bitchagno
- İlhami Çelik
- Victor Kuete
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/32388040
- DOI
- 10.1016/j.phymed.2020.153215
- eISSN
- 1618-095X
- Zeitschrift
- Phytomedicine
- Schlüsselwörter
- 8, 8-bis-(dihydroconiferyl)-diferulate
- Cell death
- Diferulic acid
- Multi-drug resistance
- Natural product
- Animals
- Antineoplastic Agents, Phytogenic
- Apoptosis
- Cell Line, Tumor
- Doxorubicin
- Drug Resistance, Multiple
- Drug Resistance, Neoplasm
- Humans
- Leukemia
- Mice
- Plant Extracts
- Sprache
- eng
- Country
- Germany
- Paginierung
- 153215
- PII
- S0944-7113(20)30047-7
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2020
- Titel
- 8,8-bis-(Dihydroconiferyl)-diferulate displayed impressive cytotoxicity towards a panel of human and animal cancer cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 70
Data source: PubMed
- Beziehungen:
- Property of