Bisphenolic compounds alter gene expression in MCF-7 cells through interaction with estrogen receptor α

Publication type:
Journal article
Metadata:
Autoren
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000538937600001&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.taap.2020.115030
eISSN
1096-0333
Externe Identifier
  • Clarivate Analytics Document Solution ID: LW1WT
  • PubMed Identifier: 32387340
ISSN
0041-008X
Zeitschrift
TOXICOLOGY AND APPLIED PHARMACOLOGY
Schlüsselwörter
  • Bisphenol
  • Tetramethyl bisphenol A
  • Estrogen receptor
  • Microplastic
  • Next generation sequencing
Artikelnummer
ARTN 115030
Datum der Veröffentlichung
2020
Status
Published
Titel
Bisphenolic compounds alter gene expression in MCF-7 cells through interaction with estrogen receptor α
Sub types
  • Article
Ausgabe der Zeitschrift
399

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
DOI
10.1016/j.taap.2020.115030
ISSN
0041-008X
Zeitschrift
Toxicology and Applied Pharmacology
Sprache
en
Artikelnummer
115030
Paginierung
115030 - 115030
Datum der Veröffentlichung
2020
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.taap.2020.115030
Datum der Datenerfassung
2020
Titel
Bisphenolic compounds alter gene expression in MCF-7 cells through interaction with estrogen receptor α
Ausgabe der Zeitschrift
399

Data source: Crossref

Abstract
Plasticizers released from microplastic are increasingly viewed with concern. While adverse health effects induced by bisphenol A and its analogues on marine animals are well documented in the literature, the endocrine potential of bisphenolic compounds on human health remains elusive. We applied next generation sequencing (NGS) with the estrogen receptor α (ERα) positive human breast cancer cell line MCF-7 treated with 17-β-estradiol (E2), bisphenol A (BPA), bisphenol B (BPB), bisphenol Z (BPZ) and tetramethyl bisphenol A (4MeBPA). We used molecular docking, microscale thermophoresis, ERα activation assay, and cell cycle experiments on MCF-7 and ERα overexpressing HEK293 cells to verify the impact of the compounds on ERα. 14 genes were found upregulated (ADORA1, DDIT4, CELSR2, FOSL2, JUN, HSPA13, IER3, IGF1R, PGR, RUNX2, SLC7A11, SLC7A2, SLC7A5, STC2) and 3 genes were downregulated (BCAS3, PHF19, PRKCD) in almost all samples. These genes are associated with cell growth, invasion, migration, apoptosis and cancer development. We further confirmed the binding, activation and proliferative effect of BPA, BPB, BPZ, and 4MeBPA on ERα. We provide evidence for the endocrine potential of bisphenolic compounds and give insights into their molecular effects in MCF-7 cells.
Addresses
  • Department of Pharmaceutical Biology, Institute of Pharmaceutical and Biomedical Sciences, Johannes Gutenberg University, Mainz, Germany.
Autoren
DOI
10.1016/j.taap.2020.115030
eISSN
1096-0333
Externe Identifier
  • PubMed Identifier: 32387340
Funding acknowledgements
  • Deutsche Forschungsgemeinschaft:
  • Johannes Gutenberg University Mainz:
Open access
false
ISSN
0041-008X
Zeitschrift
Toxicology and applied pharmacology
Schlüsselwörter
  • Cell Line
  • Cell Line, Tumor
  • Humans
  • Breast Neoplasms
  • Benzhydryl Compounds
  • Phenols
  • Estradiol
  • Estrogen Receptor alpha
  • Plasticizers
  • Estrogens
  • Signal Transduction
  • Cell Proliferation
  • Gene Expression
  • Down-Regulation
  • Up-Regulation
  • Female
  • Endocrine Disruptors
  • HEK293 Cells
  • MCF-7 Cells
Sprache
eng
Medium
Print-Electronic
Online publication date
2020
Paginierung
115030
Datum der Veröffentlichung
2020
Status
Published
Datum der Datenerfassung
2020
Titel
Bisphenolic compounds alter gene expression in MCF-7 cells through interaction with estrogen receptor α.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
399

Data source: Europe PubMed Central

Abstract
Plasticizers released from microplastic are increasingly viewed with concern. While adverse health effects induced by bisphenol A and its analogues on marine animals are well documented in the literature, the endocrine potential of bisphenolic compounds on human health remains elusive. We applied next generation sequencing (NGS) with the estrogen receptor α (ERα) positive human breast cancer cell line MCF-7 treated with 17-β-estradiol (E2), bisphenol A (BPA), bisphenol B (BPB), bisphenol Z (BPZ) and tetramethyl bisphenol A (4MeBPA). We used molecular docking, microscale thermophoresis, ERα activation assay, and cell cycle experiments on MCF-7 and ERα overexpressing HEK293 cells to verify the impact of the compounds on ERα. 14 genes were found upregulated (ADORA1, DDIT4, CELSR2, FOSL2, JUN, HSPA13, IER3, IGF1R, PGR, RUNX2, SLC7A11, SLC7A2, SLC7A5, STC2) and 3 genes were downregulated (BCAS3, PHF19, PRKCD) in almost all samples. These genes are associated with cell growth, invasion, migration, apoptosis and cancer development. We further confirmed the binding, activation and proliferative effect of BPA, BPB, BPZ, and 4MeBPA on ERα. We provide evidence for the endocrine potential of bisphenolic compounds and give insights into their molecular effects in MCF-7 cells.
Date of acceptance
2020
Autoren
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/32387340
DOI
10.1016/j.taap.2020.115030
eISSN
1096-0333
Zeitschrift
Toxicol Appl Pharmacol
Schlüsselwörter
  • Bisphenol
  • Estrogen receptor
  • Microplastic
  • Next generation sequencing
  • Tetramethyl bisphenol A
  • Benzhydryl Compounds
  • Breast Neoplasms
  • Cell Line
  • Cell Line, Tumor
  • Cell Proliferation
  • Down-Regulation
  • Endocrine Disruptors
  • Estradiol
  • Estrogen Receptor alpha
  • Estrogens
  • Female
  • Gene Expression
  • HEK293 Cells
  • Humans
  • MCF-7 Cells
  • Phenols
  • Plasticizers
  • Signal Transduction
  • Up-Regulation
Sprache
eng
Country
United States
Paginierung
115030
PII
S0041-008X(20)30154-X
Datum der Veröffentlichung
2020
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2020
Titel
Bisphenolic compounds alter gene expression in MCF-7 cells through interaction with estrogen receptor α.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
399

Data source: PubMed

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