Cytotoxicity of a naturally occuring spirostanol saponin, progenin III, towards a broad range of cancer cell lines by induction of apoptosis, autophagy and necroptosis
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Armelle T Mbaveng
- Godloves F Chi
- Gaelle S Nguenang
- Sara Abdelfatah
- Rodrigue V Tchangna Sop
- Bonaventure T Ngadjui
- Victor Kuete
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000541149300009&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.cbi.2020.109141
- eISSN
- 1872-7786
- Externe Identifier
- Clarivate Analytics Document Solution ID: LZ3SV
- PubMed Identifier: 32454006
- ISSN
- 0009-2797
- Zeitschrift
- CHEMICO-BIOLOGICAL INTERACTIONS
- Artikelnummer
- ARTN 109141
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Titel
- Cytotoxicity of a naturally occuring spirostanol saponin, progenin III, towards a broad range of cancer cell lines by induction of apoptosis, autophagy and necroptosis
- Sub types
- Article
- Ausgabe der Zeitschrift
- 326
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Armelle T Mbaveng
- Godloves F Chi
- Gaëlle S Nguenang
- Sara Abdelfatah
- Rodrigue V Tchangna Sop
- Bonaventure T Ngadjui
- Victor Kuete
- Thomas Efferth
- DOI
- 10.1016/j.cbi.2020.109141
- ISSN
- 0009-2797
- Zeitschrift
- Chemico-Biological Interactions
- Sprache
- en
- Artikelnummer
- 109141
- Paginierung
- 109141 - 109141
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.cbi.2020.109141
- Datum der Datenerfassung
- 2023
- Titel
- Cytotoxicity of a naturally occuring spirostanol saponin, progenin III, towards a broad range of cancer cell lines by induction of apoptosis, autophagy and necroptosis
- Ausgabe der Zeitschrift
- 326
Data source: Crossref
- Abstract
- This study was aimed to investigate the cytotoxic potential of a natural compound, progenin III on a broad range of cancer cell lines, including various sensitive and drug-resistant phenotypes. The cytotoxicity, progenin III-induced autophagic, ferroptotic and necroptotic cell death were evaluated by the resazurin reduction assay (RRA). Spectrophotometric analysis of caspases activity was performed using caspase-Glo assay. Flow cytometry was applied for cell cycle analysis (PI staining), apoptosis (annexin V/PI staining), mitochondrial membrane potential (MMP) (JC-1) and reactive oxygen species (ROS) (H<sub>2</sub>DCFH-DA). Progenin III and the reference molecule, doxorubicin exerted cytotoxic effects towards the 18 cancer cell lines tested including animal and human cell lines. The IC<sub>50</sub> values obtained ranged from 1.59 μM (towards CCRF-CEM leukemia cells) to 31.61 μM (against the BRAF-V600E homozygous mutant SKMel-28 melanoma cells) for progenin III. Normal sensitivity was achieved with CEM/ADR5000 cells and HCT116p53<sup>-/-</sup> adenocarcinoma cells respectively compared to their sensitive congeners CCRF-CEM cells and HCT116 p53<sup>+/+</sup> cells. Progenin III induced apoptosis in CCRF-CEM cells mediated by caspases 3/7 activation, MMP alteration and increase ROS production, and otherwise autophagy and necroptosis. Progenin III is a potential anticancer molecule that deserves further investigations to develop a novel drug to combat malignant diseases including refractory cancers.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 5, 55128, Mainz, Germany; Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon. Electronic address: armbatsa@yahoo.fr.
- Autoren
- Armelle T Mbaveng
- Godloves F Chi
- Gaëlle S Nguenang
- Sara Abdelfatah
- Rodrigue V Tchangna Sop
- Bonaventure T Ngadjui
- Victor Kuete
- Thomas Efferth
- DOI
- 10.1016/j.cbi.2020.109141
- eISSN
- 1872-7786
- Externe Identifier
- PubMed Identifier: 32454006
- Open access
- false
- ISSN
- 0009-2797
- Zeitschrift
- Chemico-biological interactions
- Schlüsselwörter
- Cell Line, Tumor
- HCT116 Cells
- Humans
- Melanoma, Experimental
- Reactive Oxygen Species
- Doxorubicin
- Spirostans
- Caspases
- Saponins
- Plant Extracts
- Antineoplastic Agents, Phytogenic
- Cell Cycle
- Cell Death
- Apoptosis
- Drug Resistance, Neoplasm
- Autophagy
- Membrane Potential, Mitochondrial
- Hep G2 Cells
- Necroptosis
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2020
- Paginierung
- 109141
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Datum der Datenerfassung
- 2020
- Titel
- Cytotoxicity of a naturally occuring spirostanol saponin, progenin III, towards a broad range of cancer cell lines by induction of apoptosis, autophagy and necroptosis.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 326
Data source: Europe PubMed Central
- Abstract
- This study was aimed to investigate the cytotoxic potential of a natural compound, progenin III on a broad range of cancer cell lines, including various sensitive and drug-resistant phenotypes. The cytotoxicity, progenin III-induced autophagic, ferroptotic and necroptotic cell death were evaluated by the resazurin reduction assay (RRA). Spectrophotometric analysis of caspases activity was performed using caspase-Glo assay. Flow cytometry was applied for cell cycle analysis (PI staining), apoptosis (annexin V/PI staining), mitochondrial membrane potential (MMP) (JC-1) and reactive oxygen species (ROS) (H2DCFH-DA). Progenin III and the reference molecule, doxorubicin exerted cytotoxic effects towards the 18 cancer cell lines tested including animal and human cell lines. The IC50 values obtained ranged from 1.59 μM (towards CCRF-CEM leukemia cells) to 31.61 μM (against the BRAF-V600E homozygous mutant SKMel-28 melanoma cells) for progenin III. Normal sensitivity was achieved with CEM/ADR5000 cells and HCT116p53-/- adenocarcinoma cells respectively compared to their sensitive congeners CCRF-CEM cells and HCT116 p53+/+ cells. Progenin III induced apoptosis in CCRF-CEM cells mediated by caspases 3/7 activation, MMP alteration and increase ROS production, and otherwise autophagy and necroptosis. Progenin III is a potential anticancer molecule that deserves further investigations to develop a novel drug to combat malignant diseases including refractory cancers.
- Date of acceptance
- 2020
- Autoren
- Armelle T Mbaveng
- Godloves F Chi
- Gaëlle S Nguenang
- Sara Abdelfatah
- Rodrigue V Tchangna Sop
- Bonaventure T Ngadjui
- Victor Kuete
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/32454006
- DOI
- 10.1016/j.cbi.2020.109141
- eISSN
- 1872-7786
- Zeitschrift
- Chem Biol Interact
- Schlüsselwörter
- Cell death
- Multi-drug resistance
- Natural product
- Progenin III
- Steroidal saponin
- Antineoplastic Agents, Phytogenic
- Apoptosis
- Autophagy
- Caspases
- Cell Cycle
- Cell Death
- Cell Line, Tumor
- Doxorubicin
- Drug Resistance, Neoplasm
- HCT116 Cells
- Hep G2 Cells
- Humans
- Melanoma, Experimental
- Membrane Potential, Mitochondrial
- Necroptosis
- Plant Extracts
- Reactive Oxygen Species
- Saponins
- Spirostans
- Sprache
- eng
- Country
- Ireland
- Paginierung
- 109141
- PII
- S0009-2797(20)30593-7
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2020
- Titel
- Cytotoxicity of a naturally occuring spirostanol saponin, progenin III, towards a broad range of cancer cell lines by induction of apoptosis, autophagy and necroptosis.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 326
Data source: PubMed
- Beziehungen:
- Property of