N-acetylglycoside of oleanolic acid (aridanin) displays promising cytotoxicity towards human and animal cancer cells, inducing apoptotic, ferroptotic and necroptotic cell death
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Armelle T Mbaveng
- Godloves F Chi
- Idrios N Bonsou
- Sara Abdelfatah
- Alfred N Tamfu
- Elisabeth MO Yeboah
- Victor Kuete
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000556569600019&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.phymed.2020.153261
- eISSN
- 1618-095X
- Externe Identifier
- Clarivate Analytics Document Solution ID: MV7YZ
- PubMed Identifier: 32559584
- ISSN
- 0944-7113
- Zeitschrift
- PHYTOMEDICINE
- Schlüsselwörter
- Apoptosis, Fabaceae
- Ferroptosis
- Multidrug resistance, Natural product
- Necroptosis
- Artikelnummer
- ARTN 153261
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Titel
- <i>N</i>-acetylglycoside of oleanolic acid (aridanin) displays promising cytotoxicity towards human and animal cancer cells, inducing apoptotic, ferroptotic and necroptotic cell death
- Sub types
- Article
- Ausgabe der Zeitschrift
- 76
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Armelle T Mbaveng
- Godloves F Chi
- Idrios N Bonsou
- Sara Abdelfatah
- Alfred N Tamfu
- Elisabeth MO Yeboah
- Victor Kuete
- Thomas Efferth
- DOI
- 10.1016/j.phymed.2020.153261
- ISSN
- 0944-7113
- Zeitschrift
- Phytomedicine
- Sprache
- en
- Artikelnummer
- 153261
- Paginierung
- 153261 - 153261
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.phymed.2020.153261
- Datum der Datenerfassung
- 2023
- Titel
- N-acetylglycoside of oleanolic acid (aridanin) displays promising cytotoxicity towards human and animal cancer cells, inducing apoptotic, ferroptotic and necroptotic cell death
- Ausgabe der Zeitschrift
- 76
Data source: Crossref
- Abstract
- <h4>Background</h4>The discovery of novel phytochemicals represents a reasonable approach to fight malignancies, especially those which are resistant to standard chemotherapy.<h4>Purpose</h4>We evaluated the cytotoxic potential of a naturally occurring N-acetylglycoside of oleanolic acid, aridanin, on 18 cancer cell lines, including sensitive and drug-resistant phenotypes mediated by P-glycoprotein, BCRP, p53 knockout, deletion-mutated EGFR, or BRAF mutations. Furthermore, metastasizing B16/F10 cells, HepG2 hepatocarcinoma and normal AML12 hepatocytes were investigated. The mechanisms of aridanin-induced cell death was further investigated.<h4>Methods</h4>The resazurin reduction assay (RRA) was applied to evaluate the cytotoxicity, autophagy, ferroptotic and necroptotic cell death. CCRF-CEM leukemia cells were used for all mechanistic studies. A caspase-Glo assay was applied to evaluate the caspase activities. Flow cytometry was applied for the analyses of cell cycle (PI staining), apoptosis (annexin V/PI staining), mitochondrial membrane potential (MMP; JC-1) and reactive oxygen species (ROS; H<sub>2</sub>DCFH-DA).<h4>Results</h4>Aridanin and doxorubicin (positive control) inhibited the proliferation of all cancer cell lines tested. The IC<sub>50</sub> values for aridanin varied from 3.18 µM (CCRF-CEM cells) to 9.56 µM (HepG2 cells). Aridanin had considerably lower IC<sub>50</sub> values than that of doxorubicin against multidrug-resistant CEM/ADR5000 cells and melanoma cell lines (MaMel-80a, Mel-2a, MV3, and SKMel-505). Aridanin induced apoptosis in CCRF-CEM cells through increase of ROS levels and MMP breakdown, and to a lesser extent via caspases activation. Aridanin also induced ferroptotic and necroptotic cell death.<h4>Conclusion</h4>The present study opens good perpectives for the use of this phytochemical as an anticancer drug to combat multi-facorial resistance to established chemotherapeutics.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany; Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon. Electronic address: armbatsa@yahoo.fr.
- Autoren
- Armelle T Mbaveng
- Godloves F Chi
- Idrios N Bonsou
- Sara Abdelfatah
- Alfred N Tamfu
- Elisabeth MO Yeboah
- Victor Kuete
- Thomas Efferth
- DOI
- 10.1016/j.phymed.2020.153261
- eISSN
- 1618-095X
- Externe Identifier
- PubMed Identifier: 32559584
- Open access
- false
- ISSN
- 0944-7113
- Zeitschrift
- Phytomedicine : international journal of phytotherapy and phytopharmacology
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2020
- Paginierung
- 153261
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Datum der Datenerfassung
- 2020
- Titel
- N-acetylglycoside of oleanolic acid (aridanin) displays promising cytotoxicity towards human and animal cancer cells, inducing apoptotic, ferroptotic and necroptotic cell death.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 76
Data source: Europe PubMed Central
- Abstract
- BACKGROUND: The discovery of novel phytochemicals represents a reasonable approach to fight malignancies, especially those which are resistant to standard chemotherapy. PURPOSE: We evaluated the cytotoxic potential of a naturally occurring N-acetylglycoside of oleanolic acid, aridanin, on 18 cancer cell lines, including sensitive and drug-resistant phenotypes mediated by P-glycoprotein, BCRP, p53 knockout, deletion-mutated EGFR, or BRAF mutations. Furthermore, metastasizing B16/F10 cells, HepG2 hepatocarcinoma and normal AML12 hepatocytes were investigated. The mechanisms of aridanin-induced cell death was further investigated. METHODS: The resazurin reduction assay (RRA) was applied to evaluate the cytotoxicity, autophagy, ferroptotic and necroptotic cell death. CCRF-CEM leukemia cells were used for all mechanistic studies. A caspase-Glo assay was applied to evaluate the caspase activities. Flow cytometry was applied for the analyses of cell cycle (PI staining), apoptosis (annexin V/PI staining), mitochondrial membrane potential (MMP; JC-1) and reactive oxygen species (ROS; H2DCFH-DA). RESULTS: Aridanin and doxorubicin (positive control) inhibited the proliferation of all cancer cell lines tested. The IC50 values for aridanin varied from 3.18 µM (CCRF-CEM cells) to 9.56 µM (HepG2 cells). Aridanin had considerably lower IC50 values than that of doxorubicin against multidrug-resistant CEM/ADR5000 cells and melanoma cell lines (MaMel-80a, Mel-2a, MV3, and SKMel-505). Aridanin induced apoptosis in CCRF-CEM cells through increase of ROS levels and MMP breakdown, and to a lesser extent via caspases activation. Aridanin also induced ferroptotic and necroptotic cell death. CONCLUSION: The present study opens good perpectives for the use of this phytochemical as an anticancer drug to combat multi-facorial resistance to established chemotherapeutics.
- Date of acceptance
- 2020
- Autoren
- Armelle T Mbaveng
- Godloves F Chi
- Idrios N Bonsou
- Sara Abdelfatah
- Alfred N Tamfu
- Elisabeth MO Yeboah
- Victor Kuete
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/32559584
- DOI
- 10.1016/j.phymed.2020.153261
- eISSN
- 1618-095X
- Zeitschrift
- Phytomedicine
- Schlüsselwörter
- Apoptosis, Fabaceae
- Ferroptosis
- Multidrug resistance, Natural product
- Necroptosis
- Sprache
- eng
- Country
- Germany
- Paginierung
- 153261
- PII
- S0944-7113(20)30092-1
- Datum der Veröffentlichung
- 2020
- Status
- Published online
- Titel
- N-acetylglycoside of oleanolic acid (aridanin) displays promising cytotoxicity towards human and animal cancer cells, inducing apoptotic, ferroptotic and necroptotic cell death.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 76
Data source: PubMed
- Beziehungen:
- Property of