Growth factors-based beneficial effects of platelet lysate on umbilical cord-derived stem cells and their synergistic use in osteoarthritis treatment
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Li Yan
- Li Zhou
- Bo Yan
- Lei Zhang
- Wenxi Du
- Fucun Liu
- Qiang Yuan
- Peijian Tong
- Letian Shan
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000581610800005&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1038/s41419-020-03045-0
- Externe Identifier
- Clarivate Analytics Document Solution ID: OG0UQ
- PubMed Identifier: 33057008
- ISSN
- 2041-4889
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- CELL DEATH & DISEASE
- Artikelnummer
- ARTN 857
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Titel
- Growth factors-based beneficial effects of platelet lysate on umbilical cord-derived stem cells and their synergistic use in osteoarthritis treatment
- Sub types
- Article
- Ausgabe der Zeitschrift
- 11
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:p>Poor viability of mesenchymal stem cells (MSCs) at the transplanted site often hinders the efficacy of MSCs-based therapy. Platelet lysate (PL) contains rich amounts of growth factors, which benefits cell growth. This study aimed to explore how human PL benefits umbilical cord-derived MSCs (huc-MSCs), and whether they have synergistic potential in osteoarthritis (OA) treatment. As quality control, flow cytometry and specific staining were performed to identify huc-MSCs, and ELISA was used to quantify growth factors in PL. CCK-8 and flow cytometry assays were performed to evaluate the effects of PL on the cell viability and cell cycle progression of huc-MSCs. Wound healing and transwell assays were conducted to assess the migration of huc-MSCs. RNA sequencing, real time PCR, and Western blot assays were conducted to explore the growth factors-based mechanism of PL. The in vitro results showed that PL significantly promoted the proliferation, cell cycle, and migration of huc-MSCs by upregulating relevant genes/proteins and activating beclin1-dependent autophagy via the AMPK/mTOR signaling pathway. The main growth factors (PDGF-AA, IGF-1, TGF-β, EGF, and FGF) contributed to the effects of PL in varying degrees. The in vivo data showed that combined PL and huc-MSCs exerted significant synergistic effect against OA. The overall study determined the beneficial effects and mechanism of PL on huc-MSCs and indicated PL as an adjuvant for huc-MSCs in treating OA. This is the first report on the growth factors-based mechanism of PL on huc-MSCs and their synergistic application. It provides novel knowledge of PLʹs roles and offers a promising strategy for stem cell-based OA therapy by combining PL and huc-MSCs.</jats:p>
- Autoren
- Li Yan
- Li Zhou
- Bo Yan
- Lei Zhang
- Wenxi Du
- Fucun Liu
- Qiang Yuan
- Peijian Tong
- Letian Shan
- Thomas Efferth
- DOI
- 10.1038/s41419-020-03045-0
- eISSN
- 2041-4889
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- Cell Death & Disease
- Sprache
- en
- Artikelnummer
- 857
- Online publication date
- 2020
- Status
- Published online
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1038/s41419-020-03045-0
- Datum der Datenerfassung
- 2022
- Titel
- Growth factors-based beneficial effects of platelet lysate on umbilical cord-derived stem cells and their synergistic use in osteoarthritis treatment
- Ausgabe der Zeitschrift
- 11
Data source: Crossref
- Abstract
- Poor viability of mesenchymal stem cells (MSCs) at the transplanted site often hinders the efficacy of MSCs-based therapy. Platelet lysate (PL) contains rich amounts of growth factors, which benefits cell growth. This study aimed to explore how human PL benefits umbilical cord-derived MSCs (huc-MSCs), and whether they have synergistic potential in osteoarthritis (OA) treatment. As quality control, flow cytometry and specific staining were performed to identify huc-MSCs, and ELISA was used to quantify growth factors in PL. CCK-8 and flow cytometry assays were performed to evaluate the effects of PL on the cell viability and cell cycle progression of huc-MSCs. Wound healing and transwell assays were conducted to assess the migration of huc-MSCs. RNA sequencing, real time PCR, and Western blot assays were conducted to explore the growth factors-based mechanism of PL. The in vitro results showed that PL significantly promoted the proliferation, cell cycle, and migration of huc-MSCs by upregulating relevant genes/proteins and activating beclin1-dependent autophagy via the AMPK/mTOR signaling pathway. The main growth factors (PDGF-AA, IGF-1, TGF-β, EGF, and FGF) contributed to the effects of PL in varying degrees. The in vivo data showed that combined PL and huc-MSCs exerted significant synergistic effect against OA. The overall study determined the beneficial effects and mechanism of PL on huc-MSCs and indicated PL as an adjuvant for huc-MSCs in treating OA. This is the first report on the growth factors-based mechanism of PL on huc-MSCs and their synergistic application. It provides novel knowledge of PL's roles and offers a promising strategy for stem cell-based OA therapy by combining PL and huc-MSCs.
- Addresses
- The First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, China.
- Autoren
- Li Yan
- Li Zhou
- Bo Yan
- Lei Zhang
- Wenxi Du
- Fucun Liu
- Qiang Yuan
- Peijian Tong
- Letian Shan
- Thomas Efferth
- DOI
- 10.1038/s41419-020-03045-0
- eISSN
- 2041-4889
- Externe Identifier
- PubMed Identifier: 33057008
- PubMed Central ID: PMC7560841
- Funding acknowledgements
- National Natural Science Foundation of China: 81774331
- National Natural Science Foundation of China: 81873049
- Open access
- true
- ISSN
- 2041-4889
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- Cell death & disease
- Schlüsselwörter
- Blood Platelets
- Stem Cells
- Mesenchymal Stem Cells
- Umbilical Cord
- Humans
- Osteoarthritis
- Intercellular Signaling Peptides and Proteins
- Cord Blood Stem Cell Transplantation
- Autophagy
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2020
- Open access status
- Open Access
- Paginierung
- 857
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2020
- Titel
- Growth factors-based beneficial effects of platelet lysate on umbilical cord-derived stem cells and their synergistic use in osteoarthritis treatment.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 11
Files
https://www.nature.com/articles/s41419-020-03045-0.pdf https://europepmc.org/articles/PMC7560841?pdf=render
Data source: Europe PubMed Central
- Abstract
- Poor viability of mesenchymal stem cells (MSCs) at the transplanted site often hinders the efficacy of MSCs-based therapy. Platelet lysate (PL) contains rich amounts of growth factors, which benefits cell growth. This study aimed to explore how human PL benefits umbilical cord-derived MSCs (huc-MSCs), and whether they have synergistic potential in osteoarthritis (OA) treatment. As quality control, flow cytometry and specific staining were performed to identify huc-MSCs, and ELISA was used to quantify growth factors in PL. CCK-8 and flow cytometry assays were performed to evaluate the effects of PL on the cell viability and cell cycle progression of huc-MSCs. Wound healing and transwell assays were conducted to assess the migration of huc-MSCs. RNA sequencing, real time PCR, and Western blot assays were conducted to explore the growth factors-based mechanism of PL. The in vitro results showed that PL significantly promoted the proliferation, cell cycle, and migration of huc-MSCs by upregulating relevant genes/proteins and activating beclin1-dependent autophagy via the AMPK/mTOR signaling pathway. The main growth factors (PDGF-AA, IGF-1, TGF-β, EGF, and FGF) contributed to the effects of PL in varying degrees. The in vivo data showed that combined PL and huc-MSCs exerted significant synergistic effect against OA. The overall study determined the beneficial effects and mechanism of PL on huc-MSCs and indicated PL as an adjuvant for huc-MSCs in treating OA. This is the first report on the growth factors-based mechanism of PL on huc-MSCs and their synergistic application. It provides novel knowledge of PL's roles and offers a promising strategy for stem cell-based OA therapy by combining PL and huc-MSCs.
- Date of acceptance
- 2020
- Autoren
- Li Yan
- Li Zhou
- Bo Yan
- Lei Zhang
- Wenxi Du
- Fucun Liu
- Qiang Yuan
- Peijian Tong
- Letian Shan
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/33057008
- DOI
- 10.1038/s41419-020-03045-0
- eISSN
- 2041-4889
- Externe Identifier
- PubMed Central ID: PMC7560841
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- Cell Death Dis
- Schlüsselwörter
- Autophagy
- Blood Platelets
- Cord Blood Stem Cell Transplantation
- Humans
- Intercellular Signaling Peptides and Proteins
- Mesenchymal Stem Cells
- Osteoarthritis
- Stem Cells
- Umbilical Cord
- Sprache
- eng
- Country
- England
- Paginierung
- 857
- PII
- 10.1038/s41419-020-03045-0
- Datum der Veröffentlichung
- 2020
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2021
- Titel
- Growth factors-based beneficial effects of platelet lysate on umbilical cord-derived stem cells and their synergistic use in osteoarthritis treatment.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 11
Data source: PubMed
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