Cytotoxicity and apoptosis induction by Fumaria officinalis extracts in leukemia and multiple myeloma cell lines

Publication type:
Journal article
Metadata:
Autoren
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000598059400007&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.jep.2020.113458
eISSN
1872-7573
Externe Identifier
  • Clarivate Analytics Document Solution ID: PE0JI
  • PubMed Identifier: 33039632
ISSN
0378-8741
Zeitschrift
JOURNAL OF ETHNOPHARMACOLOGY
Schlüsselwörter
  • Apoptosis
  • Autophagy
  • Ferroptosis
  • Fumaria officinalis
  • Multiple myeloma
  • Phytotherapy
Artikelnummer
ARTN 113458
Datum der Veröffentlichung
2021
Status
Published
Titel
Cytotoxicity and apoptosis induction by <i>Fumaria officinalis</i> extracts in leukemia and multiple myeloma cell lines
Sub types
  • Article
Ausgabe der Zeitschrift
266

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
DOI
10.1016/j.jep.2020.113458
ISSN
0378-8741
Zeitschrift
Journal of Ethnopharmacology
Sprache
en
Artikelnummer
113458
Paginierung
113458 - 113458
Datum der Veröffentlichung
2021
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.jep.2020.113458
Datum der Datenerfassung
2022
Titel
Cytotoxicity and apoptosis induction by Fumaria officinalis extracts in leukemia and multiple myeloma cell lines
Ausgabe der Zeitschrift
266

Data source: Crossref

Abstract
<h4>Ethnopharmacological relevance</h4>Fumaria officinalis (Fumariaceae) is recorded in the Kurdish ethnobotany for various health problems.<h4>Aim of the study</h4>In this study, the cytotoxic activity of F. officinalis extracts on two leukemia and nine multiple myeloma (MM) cell lines was investigated.<h4>Materials and methods</h4>The cytotoxic and ferroptotic activity were examined by resazurin reduction assay. Flow cytometry, immunoblotting assay and fluorescence microscopy were used to measure cell cycle distribution, apoptosis, induction of reactive oxygen species (ROS), loss integrity of mitochondrial membrane potential (MMP) and autophagy. LC-ESI/MS was used to identify chemical constituents present in F. officinalis.<h4>Results</h4>Chloroform (CF) and ethyl acetate (EF) fractions showed drastic cytotoxic effect on CCRF-CEM and CEM/ADR 5000 cells. NCI-H929 cell line exhibited higher sensitivity against CF, while EF demonstrated its higher cytotoxicity on OPM-2 cells with IC<sub>50</sub> value 14.80 ± 1.70 and 28.13 ± 1.38 μg/mL respectively. Flow cytometric and morphological studies confirmed that CF and EF induced apoptosis in NCI-H929 cells by loss of MMP, generation of ROS and obvious morphological variations. In DNA histograms, up to 50% of the cells were accumulated by CF and 44% by EF in the sub-G0/G1 phase following 72 h treatment. EF induced autophagic cell death, while CF stimulated iron-dependent cell death. Moreover, two isoquinoline alkaloids and four flavonoids were identified in the active fractions.<h4>Conclusion</h4>To our knowledge, this is the first report demonstrating the cytotoxicity of F. officinalis extracts in MM cell lines. CF and EF fractions inhibited MM cell proliferation through various modes of actions.
Addresses
  • Department of Pharmacognosy, College of Pharmacy, Hawler Medical University, Erbil, Kurdistan Region, Iraq; Department of Pharmaceutical Biology, Institute of Pharmaceutical and Biomedical Sciences, Johannes Gutenberg University, Staudinger Weg 5, 55128, Mainz, Germany.
Autoren
DOI
10.1016/j.jep.2020.113458
eISSN
1872-7573
Externe Identifier
  • PubMed Identifier: 33039632
Open access
false
ISSN
0378-8741
Zeitschrift
Journal of ethnopharmacology
Schlüsselwörter
  • Cell Line, Tumor
  • Humans
  • Fumaria
  • Leukemia
  • Multiple Myeloma
  • Reactive Oxygen Species
  • Plant Extracts
  • Antineoplastic Agents, Phytogenic
  • Inhibitory Concentration 50
  • Apoptosis
  • Autophagy
  • Membrane Potential, Mitochondrial
Sprache
eng
Medium
Print-Electronic
Online publication date
2020
Paginierung
113458
Datum der Veröffentlichung
2021
Status
Published
Datum der Datenerfassung
2020
Titel
Cytotoxicity and apoptosis induction by Fumaria officinalis extracts in leukemia and multiple myeloma cell lines.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
266

Data source: Europe PubMed Central

Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Fumaria officinalis (Fumariaceae) is recorded in the Kurdish ethnobotany for various health problems. AIM OF THE STUDY: In this study, the cytotoxic activity of F. officinalis extracts on two leukemia and nine multiple myeloma (MM) cell lines was investigated. MATERIALS AND METHODS: The cytotoxic and ferroptotic activity were examined by resazurin reduction assay. Flow cytometry, immunoblotting assay and fluorescence microscopy were used to measure cell cycle distribution, apoptosis, induction of reactive oxygen species (ROS), loss integrity of mitochondrial membrane potential (MMP) and autophagy. LC-ESI/MS was used to identify chemical constituents present in F. officinalis. RESULTS: Chloroform (CF) and ethyl acetate (EF) fractions showed drastic cytotoxic effect on CCRF-CEM and CEM/ADR 5000 cells. NCI-H929 cell line exhibited higher sensitivity against CF, while EF demonstrated its higher cytotoxicity on OPM-2 cells with IC50 value 14.80 ± 1.70 and 28.13 ± 1.38 μg/mL respectively. Flow cytometric and morphological studies confirmed that CF and EF induced apoptosis in NCI-H929 cells by loss of MMP, generation of ROS and obvious morphological variations. In DNA histograms, up to 50% of the cells were accumulated by CF and 44% by EF in the sub-G0/G1 phase following 72 h treatment. EF induced autophagic cell death, while CF stimulated iron-dependent cell death. Moreover, two isoquinoline alkaloids and four flavonoids were identified in the active fractions. CONCLUSION: To our knowledge, this is the first report demonstrating the cytotoxicity of F. officinalis extracts in MM cell lines. CF and EF fractions inhibited MM cell proliferation through various modes of actions.
Date of acceptance
2020
Autoren
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/33039632
DOI
10.1016/j.jep.2020.113458
eISSN
1872-7573
Zeitschrift
J Ethnopharmacol
Schlüsselwörter
  • Apoptosis
  • Autophagy
  • Ferroptosis
  • Fumaria officinalis
  • Multiple myeloma
  • Phytotherapy
  • Antineoplastic Agents, Phytogenic
  • Apoptosis
  • Autophagy
  • Cell Line, Tumor
  • Fumaria
  • Humans
  • Inhibitory Concentration 50
  • Leukemia
  • Membrane Potential, Mitochondrial
  • Multiple Myeloma
  • Plant Extracts
  • Reactive Oxygen Species
Sprache
eng
Country
Ireland
Paginierung
113458
PII
S0378-8741(20)33344-4
Datum der Veröffentlichung
2021
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2021
Titel
Cytotoxicity and apoptosis induction by Fumaria officinalis extracts in leukemia and multiple myeloma cell lines.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
266

Data source: PubMed

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