Cytotoxicity of botanicals and isolated phytochemicals from Araliopsis soyauxii Engl. (Rutaceae) towards a panel of human cancer cells

Publication type:
Journal article
Metadata:
Autoren
  • Armelle T Mbaveng
  • Cedric GT Noulala
  • Anne RM Samba
  • Simplice B Tankeo
  • Ghislain W Fotso
  • Emmanuel N Happi
  • Bonaventure T Ngadjui
  • Veronique P Beng
  • Victor Kuete
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000606376800006&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.jep.2020.113535
eISSN
1872-7573
Externe Identifier
  • Clarivate Analytics Document Solution ID: PQ2JV
  • PubMed Identifier: 33166626
ISSN
0378-8741
Zeitschrift
JOURNAL OF ETHNOPHARMACOLOGY
Schlüsselwörter
  • Apoptosis
  • Araliopsis soyauxii
  • Cytotoxicity
  • Multidrug resistance
  • Kihadanin B
  • Rutaceae
Artikelnummer
ARTN 113535
Datum der Veröffentlichung
2021
Status
Published
Titel
Cytotoxicity of botanicals and isolated phytochemicals from <i>Araliopsis soyauxii</i> Engl. (Rutaceae) towards a panel of human cancer cells
Sub types
  • Article
Ausgabe der Zeitschrift
267

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Armelle T Mbaveng
  • Cédric GT Noulala
  • Anne RM Samba
  • Simplice B Tankeo
  • Ghislain W Fotso
  • Emmanuel N Happi
  • Bonaventure T Ngadjui
  • Veronique P Beng
  • Victor Kuete
  • Thomas Efferth
DOI
10.1016/j.jep.2020.113535
ISSN
0378-8741
Zeitschrift
Journal of Ethnopharmacology
Sprache
en
Artikelnummer
113535
Paginierung
113535 - 113535
Datum der Veröffentlichung
2021
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.jep.2020.113535
Datum der Datenerfassung
2023
Titel
Cytotoxicity of botanicals and isolated phytochemicals from Araliopsis soyauxii Engl. (Rutaceae) towards a panel of human cancer cells
Ausgabe der Zeitschrift
267

Data source: Crossref

Abstract
<h4>Ethnopharmacological relevance</h4>Araliopsis soyauxii Engl. (Rutaceae) is a Cameroonian medicinal plant traditionally used to treat lung diseases, malaria, and gonorrhea. It has been demonstrated that infectious disease contribute to about 20% of all human tumours.<h4>Aims of the study</h4>(1) To perform a phytochemical investigation of the dichloromethane-methanol 1:1 extracts of the bark (ASB), roots (ASR), and leaves (ASL) from Araliopsis soyauxii; (2) to evaluate the cytotoxicity of extracts and isolated compounds; (3) to determine the mode of induction of apoptosis of ASB and kihadanin B (12).<h4>Materials and methods</h4>Fourteen constituents of the crude extracts were isolated by column chromatography, while spectroscopic techniques were used for structural elucidation. The resazurin reduction assay (RRA) was applied to determine the cytotoxicity of samples towards a panel of 9 cancer cell lines. For caspases activity, the Caspase-Glo assay was used; flow cytometry was applied to investigate the cell cycle distribution (PI staining), apoptosis (annexin V/PI staining), mitochondrial membrane potential (MMP; JC-1 staining), and the reactive oxygen species (ROS; H<sub>2</sub>DCFH-DA staining).<h4>Results</h4>Phytochemical investigations of botanicals (ASB, ASR, and ASL) led to the isolation of 14 compounds. Extract ASB, obacunone (11), kihadanin B (12) as well as doxorubicin (control drug) revealed cytotoxicity towards the 9 cancer cell lines tested. The IC<sub>50</sub> values ranged from 11.11 μg/mL (against CCRF-CEM leukemia cells) to 28.18 μg/mL (against HCT116 p53<sup>+/+</sup> colon adenocarcinoma cells) for ASB; from 28.25 μM (against MDA-MB-231-pcDNA breast adenocarcinoma cells) to 65.13 μM (against HepG2 hepatocarcinoma cells) for compound 11, and from 5.77 μM (against CCRF-CEM cells) to 43.56 μM (against U87.MGΔEGFR glioblastoma cells) for compound 12. ASB and compound 12 induced apoptosis in CCRF-CEM cells. ASB induced the apoptotic process mediated by MMP alteration and enhanced ROS production, while compound 12 induced apoptosis by caspases activation, MMP alteration, and enhanced ROS production.<h4>Conclusion</h4>This study demonstrated that Araliopsis soyauxii is a potential source of cytotoxic phytochemicals such as kihadanin B and that ASB and compound 12. Extract and compounds will be explored further to develop anticancer drugs.
Addresses
  • Department of Pharmaceutical Biology, Institute of Pharmaceutical and Biomedical Sciences, University of Mainz, Staudinger Weg 5, 55128, Mainz, Germany; Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon. Electronic address: armbatsa@yahoo.fr.
Autoren
  • Armelle T Mbaveng
  • Cédric GT Noulala
  • Anne RM Samba
  • Simplice B Tankeo
  • Ghislain W Fotso
  • Emmanuel N Happi
  • Bonaventure T Ngadjui
  • Veronique P Beng
  • Victor Kuete
  • Thomas Efferth
DOI
10.1016/j.jep.2020.113535
eISSN
1872-7573
Externe Identifier
  • PubMed Identifier: 33166626
Open access
false
ISSN
0378-8741
Zeitschrift
Journal of ethnopharmacology
Schlüsselwörter
  • HCT116 Cells
  • Mitochondria
  • Humans
  • Rutaceae
  • Neoplasms
  • Reactive Oxygen Species
  • Limonins
  • Benzoxepins
  • Plant Extracts
  • Antineoplastic Agents, Phytogenic
  • Inhibitory Concentration 50
  • Signal Transduction
  • Apoptosis
  • Oxidative Stress
  • Dose-Response Relationship, Drug
  • Apoptosis Regulatory Proteins
  • Membrane Potential, Mitochondrial
  • Hep G2 Cells
  • Cell Cycle Checkpoints
  • Phytochemicals
Sprache
eng
Medium
Print-Electronic
Online publication date
2020
Paginierung
113535
Datum der Veröffentlichung
2021
Status
Published
Datum der Datenerfassung
2020
Titel
Cytotoxicity of botanicals and isolated phytochemicals from Araliopsis soyauxii Engl. (Rutaceae) towards a panel of human cancer cells.
Sub types
  • Comparative Study
  • Journal Article
Ausgabe der Zeitschrift
267

Data source: Europe PubMed Central

Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Araliopsis soyauxii Engl. (Rutaceae) is a Cameroonian medicinal plant traditionally used to treat lung diseases, malaria, and gonorrhea. It has been demonstrated that infectious disease contribute to about 20% of all human tumours. AIMS OF THE STUDY: (1) To perform a phytochemical investigation of the dichloromethane-methanol 1:1 extracts of the bark (ASB), roots (ASR), and leaves (ASL) from Araliopsis soyauxii; (2) to evaluate the cytotoxicity of extracts and isolated compounds; (3) to determine the mode of induction of apoptosis of ASB and kihadanin B (12). MATERIALS AND METHODS: Fourteen constituents of the crude extracts were isolated by column chromatography, while spectroscopic techniques were used for structural elucidation. The resazurin reduction assay (RRA) was applied to determine the cytotoxicity of samples towards a panel of 9 cancer cell lines. For caspases activity, the Caspase-Glo assay was used; flow cytometry was applied to investigate the cell cycle distribution (PI staining), apoptosis (annexin V/PI staining), mitochondrial membrane potential (MMP; JC-1 staining), and the reactive oxygen species (ROS; H2DCFH-DA staining). RESULTS: Phytochemical investigations of botanicals (ASB, ASR, and ASL) led to the isolation of 14 compounds. Extract ASB, obacunone (11), kihadanin B (12) as well as doxorubicin (control drug) revealed cytotoxicity towards the 9 cancer cell lines tested. The IC50 values ranged from 11.11 μg/mL (against CCRF-CEM leukemia cells) to 28.18 μg/mL (against HCT116 p53+/+ colon adenocarcinoma cells) for ASB; from 28.25 μM (against MDA-MB-231-pcDNA breast adenocarcinoma cells) to 65.13 μM (against HepG2 hepatocarcinoma cells) for compound 11, and from 5.77 μM (against CCRF-CEM cells) to 43.56 μM (against U87.MGΔEGFR glioblastoma cells) for compound 12. ASB and compound 12 induced apoptosis in CCRF-CEM cells. ASB induced the apoptotic process mediated by MMP alteration and enhanced ROS production, while compound 12 induced apoptosis by caspases activation, MMP alteration, and enhanced ROS production. CONCLUSION: This study demonstrated that Araliopsis soyauxii is a potential source of cytotoxic phytochemicals such as kihadanin B and that ASB and compound 12. Extract and compounds will be explored further to develop anticancer drugs.
Date of acceptance
2020
Autoren
  • Armelle T Mbaveng
  • Cédric GT Noulala
  • Anne RM Samba
  • Simplice B Tankeo
  • Ghislain W Fotso
  • Emmanuel N Happi
  • Bonaventure T Ngadjui
  • Veronique P Beng
  • Victor Kuete
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/33166626
DOI
10.1016/j.jep.2020.113535
eISSN
1872-7573
Zeitschrift
J Ethnopharmacol
Schlüsselwörter
  • Apoptosis
  • Araliopsis soyauxii
  • Cytotoxicity
  • Kihadanin B
  • Multidrug resistance
  • Rutaceae
  • Antineoplastic Agents, Phytogenic
  • Apoptosis
  • Apoptosis Regulatory Proteins
  • Benzoxepins
  • Cell Cycle Checkpoints
  • Dose-Response Relationship, Drug
  • HCT116 Cells
  • Hep G2 Cells
  • Humans
  • Inhibitory Concentration 50
  • Limonins
  • Membrane Potential, Mitochondrial
  • Mitochondria
  • Neoplasms
  • Oxidative Stress
  • Phytochemicals
  • Plant Extracts
  • Reactive Oxygen Species
  • Rutaceae
  • Signal Transduction
Sprache
eng
Country
Ireland
Paginierung
113535
PII
S0378-8741(20)33423-1
Datum der Veröffentlichung
2021
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2021
Titel
Cytotoxicity of botanicals and isolated phytochemicals from Araliopsis soyauxii Engl. (Rutaceae) towards a panel of human cancer cells.
Sub types
  • Comparative Study
  • Journal Article
Ausgabe der Zeitschrift
267

Data source: PubMed

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