Induction of Apoptosis, Autophagy and Ferroptosis by Thymus vulgaris and Arctium lappa Extract in Leukemia and Multiple Myeloma Cell Lines
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Aveen N. Adham
- Mohamed Elamir F. Hegazy
- Alaadin M Naqishbandi
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000593442900001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3390/molecules25215016
- eISSN
- 1420-3049
- Externe Identifier
- Clarivate Analytics Document Solution ID: OX3AX
- PubMed Identifier: 33138135
- Ausgabe der Veröffentlichung
- 21
- Zeitschrift
- MOLECULES
- Schlüsselwörter
- apoptosis
- asteraceae
- autophagy
- cell death
- lamiaceae
- ferroptosis
- multiple myeloma
- phytotherapy
- Artikelnummer
- ARTN 5016
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Titel
- Induction of Apoptosis, Autophagy and Ferroptosis by <i>Thymus vulgaris</i> and <i>Arctium lappa</i> Extract in Leukemia and Multiple Myeloma Cell Lines
- Sub types
- Article
- Ausgabe der Zeitschrift
- 25
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:p>Thymus vulgaris and Arctium lappa have been used as a folk remedy in the Iraqi Kurdistan region to deal with different health problems. The aim of the current study is to investigate the cytotoxicity of T. vulgaris and A. lappa in leukemia and multiple myeloma (MM) cell lines and determine the mode of cell death triggered by the most potent cytotoxic fractions of both plants in MM. Resazurin assay was used to evaluate cytotoxic and ferroptosis activity, apoptosis, and modulation in the cell cycle phase were investigated via Annexin V-FITC/PI dual stain and cell-cycle arrest assays. Furthermore, we used western blotting assay for the determination of autophagy cell death. n-Hexane, chloroform, ethyl acetate, and butanol fractions of T. vulgaris and A. lappa exhibited cytotoxicity in CCRF-CEM and CEM/ADR 5000 cell lines at concentration range 0.001–100 μg/mL with potential activity revealed by chloroform and ethyl acetate fractions. NCI-H929 displayed pronounced sensitivity towards T. vulgaris (TCF) and A. lappa (ACF) chloroform fractions with IC50 values of 6.49 ± 1.48 and 21.9 ± 0.69 μg/mL, respectively. TCF induced apoptosis in NCI-H929 cells with a higher ratio (71%), compared to ACF (50%) at 4 × IC50. ACF demonstrated more potent autophagy activity than TCF. TCF and ACF induced cell cycle arrest and ferroptosis. Apigenin and nobiletin were identified in TCF, while nobiletin, ursolic acid, and lupeol were the main compounds identified in ACF. T. vulgaris and A. lappa could be considered as potential herbal drug candidates, which arrest cancer cell proliferation by induction of apoptosis, autophagic, and ferroptosis.</jats:p>
- Autoren
- Aveen N. Adham
- Mohamed Elamir F. Hegazy
- Alaadin M Naqishbandi
- Thomas Efferth
- DOI
- 10.3390/molecules25215016
- eISSN
- 1420-3049
- Ausgabe der Veröffentlichung
- 21
- Zeitschrift
- Molecules
- Sprache
- en
- Online publication date
- 2020
- Paginierung
- 5016 - 5016
- Status
- Published online
- Herausgeber
- MDPI AG
- Herausgeber URL
- http://dx.doi.org/10.3390/molecules25215016
- Datum der Datenerfassung
- 2020
- Titel
- Induction of Apoptosis, Autophagy and Ferroptosis by Thymus vulgaris and Arctium lappa Extract in Leukemia and Multiple Myeloma Cell Lines
- Ausgabe der Zeitschrift
- 25
Data source: Crossref
- Abstract
- <i>Thymus vulgaris</i> and <i>Arctium lappa</i> have been used as a folk remedy in the Iraqi Kurdistan region to deal with different health problems. The aim of the current study is to investigate the cytotoxicity of <i>T. vulgaris</i> and <i>A. lappa</i> in leukemia and multiple myeloma (MM) cell lines and determine the mode of cell death triggered by the most potent cytotoxic fractions of both plants in MM. Resazurin assay was used to evaluate cytotoxic and ferroptosis activity, apoptosis, and modulation in the cell cycle phase were investigated via Annexin V-FITC/PI dual stain and cell-cycle arrest assays. Furthermore, we used western blotting assay for the determination of autophagy cell death. <i>n</i>-Hexane, chloroform, ethyl acetate, and butanol fractions of <i>T. vulgaris</i> and <i>A. lappa</i> exhibited cytotoxicity in CCRF-CEM and CEM/ADR 5000 cell lines at concentration range 0.001-100 μg/mL with potential activity revealed by chloroform and ethyl acetate fractions. NCI-H929 displayed pronounced sensitivity towards <i>T. vulgaris</i> (TCF) and <i>A. lappa</i> (ACF) chloroform fractions with IC<sub>50</sub> values of 6.49 ± 1.48 and 21.9 ± 0.69 μg/mL, respectively. TCF induced apoptosis in NCI-H929 cells with a higher ratio (71%), compared to ACF (50%) at 4 × IC<sub>50</sub>. ACF demonstrated more potent autophagy activity than TCF. TCF and ACF induced cell cycle arrest and ferroptosis. Apigenin and nobiletin were identified in TCF, while nobiletin, ursolic acid, and lupeol were the main compounds identified in ACF. <i>T. vulgaris</i> and <i>A. lappa</i> could be considered as potential herbal drug candidates, which arrest cancer cell proliferation by induction of apoptosis, autophagic, and ferroptosis.
- Addresses
- Department of Pharmacognosy, College of Pharmacy, Hawler Medical University, Erbil 44001, Kurdistan Region, Iraq.
- Autoren
- Aveen N Adham
- Mohamed Elamir F Hegazy
- Alaadin M Naqishbandi
- Thomas Efferth
- DOI
- 10.3390/molecules25215016
- eISSN
- 1420-3049
- Externe Identifier
- PubMed Identifier: 33138135
- PubMed Central ID: PMC7663330
- Open access
- true
- ISSN
- 1420-3049
- Ausgabe der Veröffentlichung
- 21
- Zeitschrift
- Molecules (Basel, Switzerland)
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Arctium
- Thymus Plant
- Plant Leaves
- Leukemia
- Multiple Myeloma
- Plant Extracts
- Antineoplastic Agents, Phytogenic
- Apoptosis
- Autophagy
- Ferroptosis
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2020
- Open access status
- Open Access
- Paginierung
- E5016
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2020
- Titel
- Induction of Apoptosis, Autophagy and Ferroptosis by <i>Thymus vulgaris</i> and <i>Arctium lappa</i> Extract in Leukemia and Multiple Myeloma Cell Lines.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 25
Files
https://www.mdpi.com/1420-3049/25/21/5016/pdf?version=1604025282 https://europepmc.org/articles/PMC7663330?pdf=render
Data source: Europe PubMed Central
- Abstract
- Thymus vulgaris and Arctium lappa have been used as a folk remedy in the Iraqi Kurdistan region to deal with different health problems. The aim of the current study is to investigate the cytotoxicity of T. vulgaris and A. lappa in leukemia and multiple myeloma (MM) cell lines and determine the mode of cell death triggered by the most potent cytotoxic fractions of both plants in MM. Resazurin assay was used to evaluate cytotoxic and ferroptosis activity, apoptosis, and modulation in the cell cycle phase were investigated via Annexin V-FITC/PI dual stain and cell-cycle arrest assays. Furthermore, we used western blotting assay for the determination of autophagy cell death. n-Hexane, chloroform, ethyl acetate, and butanol fractions of T. vulgaris and A. lappa exhibited cytotoxicity in CCRF-CEM and CEM/ADR 5000 cell lines at concentration range 0.001-100 μg/mL with potential activity revealed by chloroform and ethyl acetate fractions. NCI-H929 displayed pronounced sensitivity towards T. vulgaris (TCF) and A. lappa (ACF) chloroform fractions with IC50 values of 6.49 ± 1.48 and 21.9 ± 0.69 μg/mL, respectively. TCF induced apoptosis in NCI-H929 cells with a higher ratio (71%), compared to ACF (50%) at 4 × IC50. ACF demonstrated more potent autophagy activity than TCF. TCF and ACF induced cell cycle arrest and ferroptosis. Apigenin and nobiletin were identified in TCF, while nobiletin, ursolic acid, and lupeol were the main compounds identified in ACF. T. vulgaris and A. lappa could be considered as potential herbal drug candidates, which arrest cancer cell proliferation by induction of apoptosis, autophagic, and ferroptosis.
- Date of acceptance
- 2020
- Autoren
- Aveen N Adham
- Mohamed Elamir F Hegazy
- Alaadin M Naqishbandi
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/33138135
- DOI
- 10.3390/molecules25215016
- eISSN
- 1420-3049
- Externe Identifier
- PubMed Central ID: PMC7663330
- Ausgabe der Veröffentlichung
- 21
- Zeitschrift
- Molecules
- Schlüsselwörter
- apoptosis
- asteraceae
- autophagy
- cell death
- ferroptosis
- lamiaceae
- multiple myeloma
- phytotherapy
- Antineoplastic Agents, Phytogenic
- Apoptosis
- Arctium
- Autophagy
- Cell Line, Tumor
- Ferroptosis
- Humans
- Leukemia
- Multiple Myeloma
- Plant Extracts
- Plant Leaves
- Thymus Plant
- Sprache
- eng
- Country
- Switzerland
- PII
- molecules25215016
- Datum der Veröffentlichung
- 2020
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2021
- Titel
- Induction of Apoptosis, Autophagy and Ferroptosis by Thymus vulgaris and Arctium lappa Extract in Leukemia and Multiple Myeloma Cell Lines.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 25
Data source: PubMed
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- Property of