The alkaloid, soyauxinium chloride, displays remarkable cytotoxic effects towards a panel of cancer cells, inducing apoptosis, ferroptosis and necroptosis
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Armelle T Mbaveng
- Cedric GT Noulala
- Anne RM Samba
- Simplice B Tankeo
- Sara Abdelfatah
- Ghislain W Fotso
- Emmanuel N Happi
- Bonaventure T Ngadjui
- Veronique P Beng
- Victor Kuete
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000600832900008&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.cbi.2020.109334
- eISSN
- 1872-7786
- Externe Identifier
- Clarivate Analytics Document Solution ID: PI1BB
- PubMed Identifier: 33245930
- ISSN
- 0009-2797
- Zeitschrift
- CHEMICO-BIOLOGICAL INTERACTIONS
- Schlüsselwörter
- Cancer
- Cell death
- Indoloquinazoline
- Multidrug resistance
- Natural product
- Artikelnummer
- ARTN 109334
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Titel
- The alkaloid, soyauxinium chloride, displays remarkable cytotoxic effects towards a panel of cancer cells, inducing apoptosis, ferroptosis and necroptosis
- Sub types
- Article
- Ausgabe der Zeitschrift
- 333
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Armelle T Mbaveng
- Cédric GT Noulala
- Anne RM Samba
- Simplice B Tankeo
- Sara Abdelfatah
- Ghislain W Fotso
- Emmanuel N Happi
- Bonaventure T Ngadjui
- Veronique P Beng
- Victor Kuete
- Thomas Efferth
- DOI
- 10.1016/j.cbi.2020.109334
- ISSN
- 0009-2797
- Zeitschrift
- Chemico-Biological Interactions
- Sprache
- en
- Artikelnummer
- 109334
- Paginierung
- 109334 - 109334
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.cbi.2020.109334
- Datum der Datenerfassung
- 2023
- Titel
- The alkaloid, soyauxinium chloride, displays remarkable cytotoxic effects towards a panel of cancer cells, inducing apoptosis, ferroptosis and necroptosis
- Ausgabe der Zeitschrift
- 333
Data source: Crossref
- Abstract
- The cytotoxic potential of a naturally occurring indoloquinazoline alkaloid, soyauxinium chloride (SCHL), was determined on a broad panel of animal and human cancer cell lines, including various sensitive and drug-resistant phenotypes. The cytotoxicity, SCHL-induced autophagic, ferroptotic, and necroptotic cell death were evaluated by the resazurin reduction assay (RRA). Caspase-Glo assay was used to detect the activity of caspases using spectrophotometric analysis. Flow cytometry was applied for cell cycle analysis (PI staining), apoptosis (annexin V/PI staining), mitochondrial membrane potential (MMP) (JC-1) and reactive oxygen species (ROS) (H<sub>2</sub>DCFH-DA). SCHL and doxorubicin (reference molecule) exhibited cytotoxic effects towards the 18 cancer cell lines tested. The IC<sub>50</sub> values obtained ranged from 3.64 μM (towards CCRF-CEM leukemia cells) to 16.86 μM (against the BRAF-wildtype SKMel-505 melanoma cells for SCHL). Collateral sensitivity of the resistant HCT116 p53<sup>-/-</sup> colon adenocarcinoma cells to SCHL was observed as well as the normal sensitivity of CEM/ADR5000 leukemia cells, MDA-MB-231-BCRP breast adenocarcinoma cells and U87. MGΔEGFR glioblastoma cells. SCHL induced apoptosis in CCRF-CEM cells via caspases 3/7-, 8- and 9-activation, MMP alteration and increased ROS production, and otherwise ferroptosis and necroptosis. SCHL is a prominent cytotoxic alkaloid that should be further studied to develop a novel drug to combat cancers including refractory phenotypes.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmaceutical and Biomedical Sciences, University of Mainz, Staudinger Weg 5, 55128, Mainz, Germany; Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon. Electronic address: armbatsa@yahoo.fr.
- Autoren
- Armelle T Mbaveng
- Cédric GT Noulala
- Anne RM Samba
- Simplice B Tankeo
- Sara Abdelfatah
- Ghislain W Fotso
- Emmanuel N Happi
- Bonaventure T Ngadjui
- Veronique P Beng
- Victor Kuete
- Thomas Efferth
- DOI
- 10.1016/j.cbi.2020.109334
- eISSN
- 1872-7786
- Externe Identifier
- PubMed Identifier: 33245930
- Funding acknowledgements
- Alexander von Humboldt-Stiftung:
- Open access
- false
- ISSN
- 0009-2797
- Zeitschrift
- Chemico-biological interactions
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Reactive Oxygen Species
- Antineoplastic Agents
- Cell Cycle
- Apoptosis
- Mitochondrial Membranes
- Necroptosis
- Ferroptosis
- Regulated Cell Death
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2020
- Paginierung
- 109334
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Datum der Datenerfassung
- 2020
- Titel
- The alkaloid, soyauxinium chloride, displays remarkable cytotoxic effects towards a panel of cancer cells, inducing apoptosis, ferroptosis and necroptosis.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 333
Data source: Europe PubMed Central
- Abstract
- The cytotoxic potential of a naturally occurring indoloquinazoline alkaloid, soyauxinium chloride (SCHL), was determined on a broad panel of animal and human cancer cell lines, including various sensitive and drug-resistant phenotypes. The cytotoxicity, SCHL-induced autophagic, ferroptotic, and necroptotic cell death were evaluated by the resazurin reduction assay (RRA). Caspase-Glo assay was used to detect the activity of caspases using spectrophotometric analysis. Flow cytometry was applied for cell cycle analysis (PI staining), apoptosis (annexin V/PI staining), mitochondrial membrane potential (MMP) (JC-1) and reactive oxygen species (ROS) (H2DCFH-DA). SCHL and doxorubicin (reference molecule) exhibited cytotoxic effects towards the 18 cancer cell lines tested. The IC50 values obtained ranged from 3.64 μM (towards CCRF-CEM leukemia cells) to 16.86 μM (against the BRAF-wildtype SKMel-505 melanoma cells for SCHL). Collateral sensitivity of the resistant HCT116 p53-/- colon adenocarcinoma cells to SCHL was observed as well as the normal sensitivity of CEM/ADR5000 leukemia cells, MDA-MB-231-BCRP breast adenocarcinoma cells and U87. MGΔEGFR glioblastoma cells. SCHL induced apoptosis in CCRF-CEM cells via caspases 3/7-, 8- and 9-activation, MMP alteration and increased ROS production, and otherwise ferroptosis and necroptosis. SCHL is a prominent cytotoxic alkaloid that should be further studied to develop a novel drug to combat cancers including refractory phenotypes.
- Date of acceptance
- 2020
- Autoren
- Armelle T Mbaveng
- Cédric GT Noulala
- Anne RM Samba
- Simplice B Tankeo
- Sara Abdelfatah
- Ghislain W Fotso
- Emmanuel N Happi
- Bonaventure T Ngadjui
- Veronique P Beng
- Victor Kuete
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/33245930
- DOI
- 10.1016/j.cbi.2020.109334
- eISSN
- 1872-7786
- Zeitschrift
- Chem Biol Interact
- Schlüsselwörter
- Cancer
- Cell death
- Indoloquinazoline
- Multidrug resistance
- Natural product
- Antineoplastic Agents
- Apoptosis
- Cell Cycle
- Cell Line, Tumor
- Ferroptosis
- Humans
- Mitochondrial Membranes
- Necroptosis
- Reactive Oxygen Species
- Regulated Cell Death
- Sprache
- eng
- Country
- Ireland
- Paginierung
- 109334
- PII
- S0009-2797(20)31410-1
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2020
- Titel
- The alkaloid, soyauxinium chloride, displays remarkable cytotoxic effects towards a panel of cancer cells, inducing apoptosis, ferroptosis and necroptosis.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 333
Data source: PubMed
- Beziehungen:
- Property of