Cytotoxic phytochemicals from the crude extract of Tetrapleura tetraptera fruits towards multi-factorial drug resistant cancer cells

Publication type:
Journal article
Metadata:
Autoren
  • Armelle T Mbaveng
  • Godloves F Chi
  • Idrios N Bonsou
  • Japheth O Ombito
  • Samuel O Yeboah
  • Victor Kuete
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000606360900004&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.jep.2020.113632
eISSN
1872-7573
Externe Identifier
  • Clarivate Analytics Document Solution ID: PQ2DT
  • PubMed Identifier: 33253828
ISSN
0378-8741
Zeitschrift
JOURNAL OF ETHNOPHARMACOLOGY
Schlüsselwörter
  • Apoptosis
  • Cancer
  • Cytotoxicity
  • Fabaceae
  • Multi-drug resistance
  • Tetrapleura tetraptera
Artikelnummer
ARTN 113632
Datum der Veröffentlichung
2021
Status
Published
Titel
Cytotoxic phytochemicals from the crude extract of <i>Tetrapleura tetraptera</i> fruits towards multi-factorial drug resistant cancer cells
Sub types
  • Article
Ausgabe der Zeitschrift
267

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Armelle T Mbaveng
  • Godloves F Chi
  • Idrios N Bonsou
  • Japheth O Ombito
  • Samuel O Yeboah
  • Victor Kuete
  • Thomas Efferth
DOI
10.1016/j.jep.2020.113632
ISSN
0378-8741
Zeitschrift
Journal of Ethnopharmacology
Sprache
en
Artikelnummer
113632
Paginierung
113632 - 113632
Datum der Veröffentlichung
2021
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.jep.2020.113632
Datum der Datenerfassung
2023
Titel
Cytotoxic phytochemicals from the crude extract of Tetrapleura tetraptera fruits towards multi-factorial drug resistant cancer cells
Ausgabe der Zeitschrift
267

Data source: Crossref

Abstract
<h4>Ethnopharmacological relevance</h4>Tetrapleura tetraptera is an African medicinal spice used in traditional medicine to treat several ailments including cancer.<h4>Aim of the study</h4>The present study was designed to evaluate the cytotoxicity of the dichloromethane-methanol (1:1) extract of the fruits of Tetrapleura tetraptera (TTF) and its constituents: (3R, 4S)-3,4-dimethyloxetan-2-one (1), luteolin (2), stigmasterol (4), 3-O-[6'-O-undecanoyl-β-<sub>D</sub>-glucopyranosyl]stigmasterol (6), olean-12-en-3-β-O-<sub>D</sub>-glucopyranoside (7), 3-O-β-<sub>D</sub>-glucopyranosyl-(1 → 6)-β-<sub>D</sub>-glucopyranosylurs-12-en-28-oic acid (8), 3-O-β-<sub>D</sub>-glucopyranosyl-(1 → 3)-β-<sub>D</sub>-glucopyranosyl-27-hydroxyolean-12-ene-28-oic acid (9), methyl-O-β-<sub>D</sub>-glucopyranoside (10), β-<sub>D</sub>-fructofuranosyl-(2 → 1)-β-<sub>D</sub>-glucopyranoside (11) towards a panel of cancer cell lines including MDR phenotypes. The cellular mode of induction of apoptosis by TTF and compound 7 was further investigated.<h4>Materials and methods</h4>The resazurin reduction assay (RRA) was applied to determine the cytotoxicity of the studied samples. The cell cycle (PI staining), apoptosis (annexin V/PI staining), mitochondrial membrane potential (MMP; JC-1) and reactive oxygen species (ROS; H<sub>2</sub>DCFH-DA) were measured by flow cytometry. Column chromatography was used for the purification of TTF, whilst nuclear magnetic resonance (NMR) spectroscopic analysis was applied for structural elucidation.<h4>Results</h4>The botanical, TTF and the phytochemicals, 2, 7, 8 and 9 as well as doxorubicin exerted cytotoxicity against 9 cancer cell lines including drug-sensitive and drug resistant phenotypes. TTF, compound 7 and doxorubicin were the most active samples, and displayed IC<sub>50</sub> values ranging from 10.27 μg/mL (in CCRF-CEM leukemia cells) to 23.61 μg/mL (against HCT116 p53<sup>-/-</sup> colon adenocarcinoma cells) for TTF, from 4.76 μM (against CCRF-CEM cells) to 12.92 μM (against HepG2 hepatocarcinoma cells) for compound 7, and from 0.02 μM (against CCRF-CEM cells) to 122.96 μM (against CEM/ADR5000 cells) for doxorubicin. TTF induced apoptosis in CCRF-CEM cells through MMP alteration and increased ROS production while compound 7 induced apoptosis mediated by caspases activation, MMP alteration and increased ROS production.<h4>Conclusion</h4>Tetrapleura tetraptera and some of its constituents, mostly compound 7 are good cytotoxic natural products that should be explored in depth to develop new drugs to fight cancers.
Addresses
  • Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 5, 55128, Mainz, Germany; Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon. Electronic address: armbatsa@yahoo.fr.
Autoren
  • Armelle T Mbaveng
  • Godloves F Chi
  • Idrios N Bonsou
  • Japheth O Ombito
  • Samuel O Yeboah
  • Victor Kuete
  • Thomas Efferth
DOI
10.1016/j.jep.2020.113632
eISSN
1872-7573
Externe Identifier
  • PubMed Identifier: 33253828
Open access
false
ISSN
0378-8741
Zeitschrift
Journal of ethnopharmacology
Schlüsselwörter
  • HCT116 Cells
  • Humans
  • Tetrapleura
  • Fruit
  • Neoplasms
  • Reactive Oxygen Species
  • Caspases
  • Plant Extracts
  • Antineoplastic Agents, Phytogenic
  • Inhibitory Concentration 50
  • Drug Resistance, Multiple
  • Signal Transduction
  • Apoptosis
  • Oxidative Stress
  • Dose-Response Relationship, Drug
  • Drug Resistance, Neoplasm
  • Membrane Potential, Mitochondrial
  • Hep G2 Cells
  • Phytochemicals
Sprache
eng
Medium
Print-Electronic
Online publication date
2020
Paginierung
113632
Datum der Veröffentlichung
2021
Status
Published
Datum der Datenerfassung
2020
Titel
Cytotoxic phytochemicals from the crude extract of Tetrapleura tetraptera fruits towards multi-factorial drug resistant cancer cells.
Sub types
  • Comparative Study
  • Journal Article
Ausgabe der Zeitschrift
267

Data source: Europe PubMed Central

Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Tetrapleura tetraptera is an African medicinal spice used in traditional medicine to treat several ailments including cancer. AIM OF THE STUDY: The present study was designed to evaluate the cytotoxicity of the dichloromethane-methanol (1:1) extract of the fruits of Tetrapleura tetraptera (TTF) and its constituents: (3R, 4S)-3,4-dimethyloxetan-2-one (1), luteolin (2), stigmasterol (4), 3-O-[6'-O-undecanoyl-β-D-glucopyranosyl]stigmasterol (6), olean-12-en-3-β-O-D-glucopyranoside (7), 3-O-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranosylurs-12-en-28-oic acid (8), 3-O-β-D-glucopyranosyl-(1 → 3)-β-D-glucopyranosyl-27-hydroxyolean-12-ene-28-oic acid (9), methyl-O-β-D-glucopyranoside (10), β-D-fructofuranosyl-(2 → 1)-β-D-glucopyranoside (11) towards a panel of cancer cell lines including MDR phenotypes. The cellular mode of induction of apoptosis by TTF and compound 7 was further investigated. MATERIALS AND METHODS: The resazurin reduction assay (RRA) was applied to determine the cytotoxicity of the studied samples. The cell cycle (PI staining), apoptosis (annexin V/PI staining), mitochondrial membrane potential (MMP; JC-1) and reactive oxygen species (ROS; H2DCFH-DA) were measured by flow cytometry. Column chromatography was used for the purification of TTF, whilst nuclear magnetic resonance (NMR) spectroscopic analysis was applied for structural elucidation. RESULTS: The botanical, TTF and the phytochemicals, 2, 7, 8 and 9 as well as doxorubicin exerted cytotoxicity against 9 cancer cell lines including drug-sensitive and drug resistant phenotypes. TTF, compound 7 and doxorubicin were the most active samples, and displayed IC50 values ranging from 10.27 μg/mL (in CCRF-CEM leukemia cells) to 23.61 μg/mL (against HCT116 p53-/- colon adenocarcinoma cells) for TTF, from 4.76 μM (against CCRF-CEM cells) to 12.92 μM (against HepG2 hepatocarcinoma cells) for compound 7, and from 0.02 μM (against CCRF-CEM cells) to 122.96 μM (against CEM/ADR5000 cells) for doxorubicin. TTF induced apoptosis in CCRF-CEM cells through MMP alteration and increased ROS production while compound 7 induced apoptosis mediated by caspases activation, MMP alteration and increased ROS production. CONCLUSION: Tetrapleura tetraptera and some of its constituents, mostly compound 7 are good cytotoxic natural products that should be explored in depth to develop new drugs to fight cancers.
Date of acceptance
2020
Autoren
  • Armelle T Mbaveng
  • Godloves F Chi
  • Idrios N Bonsou
  • Japheth O Ombito
  • Samuel O Yeboah
  • Victor Kuete
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/33253828
DOI
10.1016/j.jep.2020.113632
eISSN
1872-7573
Zeitschrift
J Ethnopharmacol
Schlüsselwörter
  • Apoptosis
  • Cancer
  • Cytotoxicity
  • Fabaceae
  • Multi-drug resistance
  • Tetrapleura tetraptera
  • Humans
  • Antineoplastic Agents, Phytogenic
  • Apoptosis
  • Caspases
  • Dose-Response Relationship, Drug
  • Drug Resistance, Multiple
  • Drug Resistance, Neoplasm
  • Fruit
  • HCT116 Cells
  • Hep G2 Cells
  • Inhibitory Concentration 50
  • Membrane Potential, Mitochondrial
  • Neoplasms
  • Oxidative Stress
  • Phytochemicals
  • Plant Extracts
  • Reactive Oxygen Species
  • Signal Transduction
  • Tetrapleura
Sprache
eng
Country
Ireland
Paginierung
113632
PII
S0378-8741(20)33520-0
Datum der Veröffentlichung
2021
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2021
Titel
Cytotoxic phytochemicals from the crude extract of Tetrapleura tetraptera fruits towards multi-factorial drug resistant cancer cells.
Sub types
  • Comparative Study
  • Journal Article
Ausgabe der Zeitschrift
267

Data source: PubMed

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