Identification of metastasis-related genes by genomic and transcriptomic studies in murine melanoma
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Onat Kadioglu
- Mohamed EM Saeed
- Nuha Mahmoud
- Shaymaa S Hussein Azawi
- Martina Rincic
- Thomas Liehr
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000613297100018&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.lfs.2020.118922
- eISSN
- 1879-0631
- Externe Identifier
- Clarivate Analytics Document Solution ID: QA2SA
- PubMed Identifier: 33358905
- ISSN
- 0024-3205
- Zeitschrift
- LIFE SCIENCES
- Schlüsselwörter
- Carcinogenesis
- Chromosomal aberrations
- Cytogenetics
- Melanoma
- RNA-sequencing
- Transcriptomics
- Artikelnummer
- ARTN 118922
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Titel
- Identification of metastasis-related genes by genomic and transcriptomic studies in murine melanoma
- Sub types
- Article
- Ausgabe der Zeitschrift
- 267
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Onat Kadioglu
- Mohamed EM Saeed
- Nuha Mahmoud
- Shaymaa S Hussein Azawi
- Martina Rincic
- Thomas Liehr
- Thomas Efferth
- DOI
- 10.1016/j.lfs.2020.118922
- ISSN
- 0024-3205
- Zeitschrift
- Life Sciences
- Sprache
- en
- Artikelnummer
- 118922
- Paginierung
- 118922 - 118922
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.lfs.2020.118922
- Datum der Datenerfassung
- 2024
- Titel
- Identification of metastasis-related genes by genomic and transcriptomic studies in murine melanoma
- Ausgabe der Zeitschrift
- 267
Data source: Crossref
- Abstract
- <h4>Aims</h4>We systematically characterized metastatic murine B16-F10 melanoma, a sub-line derived from murine melanoma B16-F1 cells.<h4>Materials and methods</h4>RNA-sequencing and network analyses (Ingenuity Pathway Analysis) were performed to identify novel potential metastasis mechanisms. Chromosomal aberrations were identified by multicolor fluorescence in situ hybridization (mFISH) using all 21 murine whole chromosome painting probes.<h4>Key findings</h4>Numerous genes were overexpressed in B16-F10 cells, some of which have been already described as being metastasis-linked. Nr5a1/sf1, a known prognostic marker for adrenal tumors, was 177-fold upregulated in B16-F10 cells compared to B16-F1 cells. Hoxb8 was 75-fold upregulated, which was previously associated with gastric cancer progression and metastasis. Ptk7, which is linked with tumorigenesis and metastasis of esophageal squamous carcinoma, was 67-fold upregulated. B16-F10 cells acquired additional chromosomal aberrations compared to B16-F1 cells, including dic(4)(pter->qter:qter->pter), +dic(6;15), +der(10)t(10;?1;16).<h4>Significance</h4>In addition to well-known metastatic genes, numerous novel genes and genomic aberrations were identified, which may serve as targets for treatment in the future. Transcriptomic and genetic analyses in B16-F10 cells unraveled a range of novel metastasis mechanisms, which may also have important implications for future treatment strategies.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmaceutical and Biomedical Sciences, Johannes Gutenberg University, Mainz, Germany.
- Autoren
- Onat Kadioglu
- Mohamed EM Saeed
- Nuha Mahmoud
- Shaymaa S Hussein Azawi
- Martina Rincic
- Thomas Liehr
- Thomas Efferth
- DOI
- 10.1016/j.lfs.2020.118922
- eISSN
- 1879-0631
- Externe Identifier
- PubMed Identifier: 33358905
- Funding acknowledgements
- Johannes Gutenberg-Universität Mainz:
- Open access
- false
- ISSN
- 0024-3205
- Zeitschrift
- Life sciences
- Schlüsselwörter
- Cell Line, Tumor
- Animals
- Mice, Inbred C57BL
- Mice
- Melanoma
- Melanoma, Experimental
- Neoplasm Metastasis
- In Situ Hybridization, Fluorescence
- Sequence Analysis, RNA
- Genomics
- Genome
- Transcriptome
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2020
- Paginierung
- 118922
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Datum der Datenerfassung
- 2020
- Titel
- Identification of metastasis-related genes by genomic and transcriptomic studies in murine melanoma.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 267
Data source: Europe PubMed Central
- Abstract
- AIMS: We systematically characterized metastatic murine B16-F10 melanoma, a sub-line derived from murine melanoma B16-F1 cells. MATERIALS AND METHODS: RNA-sequencing and network analyses (Ingenuity Pathway Analysis) were performed to identify novel potential metastasis mechanisms. Chromosomal aberrations were identified by multicolor fluorescence in situ hybridization (mFISH) using all 21 murine whole chromosome painting probes. KEY FINDINGS: Numerous genes were overexpressed in B16-F10 cells, some of which have been already described as being metastasis-linked. Nr5a1/sf1, a known prognostic marker for adrenal tumors, was 177-fold upregulated in B16-F10 cells compared to B16-F1 cells. Hoxb8 was 75-fold upregulated, which was previously associated with gastric cancer progression and metastasis. Ptk7, which is linked with tumorigenesis and metastasis of esophageal squamous carcinoma, was 67-fold upregulated. B16-F10 cells acquired additional chromosomal aberrations compared to B16-F1 cells, including dic(4)(pter->qter:qter->pter), +dic(6;15), +der(10)t(10;?1;16). SIGNIFICANCE: In addition to well-known metastatic genes, numerous novel genes and genomic aberrations were identified, which may serve as targets for treatment in the future. Transcriptomic and genetic analyses in B16-F10 cells unraveled a range of novel metastasis mechanisms, which may also have important implications for future treatment strategies.
- Date of acceptance
- 2020
- Autoren
- Onat Kadioglu
- Mohamed EM Saeed
- Nuha Mahmoud
- Shaymaa S Hussein Azawi
- Martina Rincic
- Thomas Liehr
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/33358905
- DOI
- 10.1016/j.lfs.2020.118922
- eISSN
- 1879-0631
- Zeitschrift
- Life Sci
- Schlüsselwörter
- Carcinogenesis
- Chromosomal aberrations
- Cytogenetics
- Melanoma
- RNA-sequencing
- Transcriptomics
- Animals
- Cell Line, Tumor
- Genome
- Genomics
- In Situ Hybridization, Fluorescence
- Melanoma
- Melanoma, Experimental
- Mice
- Mice, Inbred C57BL
- Neoplasm Metastasis
- Sequence Analysis, RNA
- Transcriptome
- Sprache
- eng
- Country
- Netherlands
- Paginierung
- 118922
- PII
- S0024-3205(20)31682-9
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2021
- Titel
- Identification of metastasis-related genes by genomic and transcriptomic studies in murine melanoma.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 267
Data source: PubMed
- Beziehungen:
- Property of