Bioactivity of fractions and constituents of Piper capense fruits towards a broad panel of cancer cells
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Armelle T Mbaveng
- Brice EN Wamba
- Gabin TM Bitchagno
- Simplice Beaudelaire Tankeo
- Ilhami Celik
- Brice CK Atontsa
- Antoine H Nkuete Lonfouo
- Victor Kuete
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000625359700004&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.jep.2021.113884
- eISSN
- 1872-7573
- Externe Identifier
- Clarivate Analytics Document Solution ID: QR6XP
- PubMed Identifier: 33529639
- ISSN
- 0378-8741
- Zeitschrift
- JOURNAL OF ETHNOPHARMACOLOGY
- Schlüsselwörter
- Apoptosis
- Cancer
- Cytotoxicity
- Phytotherapy
- Piperaceae
- Multi-drug resistance
- Artikelnummer
- ARTN 113884
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Titel
- Bioactivity of fractions and constituents of <i>Piper capense</i> fruits towards a broad panel of cancer cells
- Sub types
- Article
- Ausgabe der Zeitschrift
- 271
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Armelle T Mbaveng
- Brice EN Wamba
- Gabin TM Bitchagno
- Simplice Beaudelaire Tankeo
- İlhami Çelik
- Brice CK Atontsa
- Antoine H Nkuété Lonfouo
- Victor Kuete
- Thomas Efferth
- DOI
- 10.1016/j.jep.2021.113884
- ISSN
- 0378-8741
- Zeitschrift
- Journal of Ethnopharmacology
- Sprache
- en
- Artikelnummer
- 113884
- Paginierung
- 113884 - 113884
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.jep.2021.113884
- Datum der Datenerfassung
- 2023
- Titel
- Bioactivity of fractions and constituents of Piper capense fruits towards a broad panel of cancer cells
- Ausgabe der Zeitschrift
- 271
Data source: Crossref
- Abstract
- <h4>Ethnopharmacological relevance</h4>Piper capense is a medicinal spice whose fruits are traditionally used as aqueous decoction to heal several ailments such as trypanosomiasis, helminthic infections, and cancer.<h4>Aim of the study</h4>(1) To perform phytochemical investigation of the methanol extract of Piper capense; (2) to evaluate the cytotoxicity of botanicals (PCF, fractions PCFa-e), isolated phytochemicals on a broad panel of animal and human cancer cell lines; (3) to evaluate the induction of apoptosis of the most active samples.<h4>Material and methods</h4>Resazurin reduction assay (RRA) was used to determine the cytotoxicity of the studied samples. Cell cycle distribution (PI staining), apoptosis (annexin V/PI staining), mitochondrial membrane potential (MMP; JC-1) and reactive oxygen species (ROS; H<sub>2</sub>DCFH-DA) were measured by flow cytometry. Column chromatography (CC) was used for the purification of PCF, whilst nuclear magnetic resonance (NMR) spectroscopic and mass spectrometric (MS) analyses were applied for structural elucidation.<h4>Results</h4>The phytochemical investigation of PCF led to the isolation of 11 compounds: licarin B (1), licarin A (2), 7-(1,3-benzodioxol-5-yl)-7,8-dihydro-8-methyl-5-(2-propenyl)-furo[3,2-e]-1,3-benzodioxole (3), nitidine isocyanate (4), 5-hydroxy-7,4'-dimethoxyflavone (5), cardamomin (6), sitosterol (7) and stigmasterol (8), β-sitosterol 3-O-β-<sub>D</sub>-glucopyranoside (9), oleanolic acid (10) and lupeol (11). Fraction PCFb, compound 2 and doxorubicin (as positive control drug) revealed cytotoxic effects towards the 18 tested cancer cell lines. The IC<sub>50</sub> values ranged from 6.1 μg/mL (against CCRF-CEM cells) to 44.2 μg/mL (against BRAF-V600E homozygous mutant melanoma cells) for PSCb; from 4.3 μM (against CCRF-CEM cells) to 21.8 μM (against HCT116 p53<sup>-/-</sup>) for compound 2 and from 0.02 μM (against CCRF-CEM cells) to 123.0 μM (against CEM/ADR5000 cells) for doxorubicin. PCFb and compound 2 induced apoptosis in CCRF-CEM cells mediated by activation of caspase 3/7, 8 and 9, MMP alteration and increased ROS production.<h4>Conclusion</h4>Piper capense is a source of potent cytotoxic botanicals and phytochemicals that could help to fight various types of cancer including multidrug resistance phenotypes. PCFb and compound 2 should further be explored to develop new drugs to fight malignancies.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmaceutical and Biomedical Sciences, Johannes Gutenberg University, Staudinger Weg 5, 55128, Mainz, Germany; Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon. Electronic address: armbatsa@yahoo.fr.
- Autoren
- Armelle T Mbaveng
- Brice EN Wamba
- Gabin TM Bitchagno
- Simplice Beaudelaire Tankeo
- İlhami Çelik
- Brice CK Atontsa
- Antoine H Nkuété Lonfouo
- Victor Kuete
- Thomas Efferth
- DOI
- 10.1016/j.jep.2021.113884
- eISSN
- 1872-7573
- Externe Identifier
- PubMed Identifier: 33529639
- Funding acknowledgements
- Alexander von Humboldt-Stiftung:
- Open access
- false
- ISSN
- 0378-8741
- Zeitschrift
- Journal of ethnopharmacology
- Schlüsselwörter
- Cell Line, Tumor
- Animals
- Humans
- Piper
- Fruit
- Hydrogen Peroxide
- Reactive Oxygen Species
- Lignans
- Valinomycin
- Doxorubicin
- Caspases
- Plant Extracts
- Antineoplastic Agents, Phytogenic
- Cell Cycle
- Apoptosis
- Drug Resistance, Neoplasm
- Membrane Potential, Mitochondrial
- Phytochemicals
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2021
- Paginierung
- 113884
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Datum der Datenerfassung
- 2021
- Titel
- Bioactivity of fractions and constituents of Piper capense fruits towards a broad panel of cancer cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 271
Data source: Europe PubMed Central
- Abstract
- ETHNOPHARMACOLOGICAL RELEVANCE: Piper capense is a medicinal spice whose fruits are traditionally used as aqueous decoction to heal several ailments such as trypanosomiasis, helminthic infections, and cancer. AIM OF THE STUDY: (1) To perform phytochemical investigation of the methanol extract of Piper capense; (2) to evaluate the cytotoxicity of botanicals (PCF, fractions PCFa-e), isolated phytochemicals on a broad panel of animal and human cancer cell lines; (3) to evaluate the induction of apoptosis of the most active samples. MATERIAL AND METHODS: Resazurin reduction assay (RRA) was used to determine the cytotoxicity of the studied samples. Cell cycle distribution (PI staining), apoptosis (annexin V/PI staining), mitochondrial membrane potential (MMP; JC-1) and reactive oxygen species (ROS; H2DCFH-DA) were measured by flow cytometry. Column chromatography (CC) was used for the purification of PCF, whilst nuclear magnetic resonance (NMR) spectroscopic and mass spectrometric (MS) analyses were applied for structural elucidation. RESULTS: The phytochemical investigation of PCF led to the isolation of 11 compounds: licarin B (1), licarin A (2), 7-(1,3-benzodioxol-5-yl)-7,8-dihydro-8-methyl-5-(2-propenyl)-furo[3,2-e]-1,3-benzodioxole (3), nitidine isocyanate (4), 5-hydroxy-7,4'-dimethoxyflavone (5), cardamomin (6), sitosterol (7) and stigmasterol (8), β-sitosterol 3-O-β-D-glucopyranoside (9), oleanolic acid (10) and lupeol (11). Fraction PCFb, compound 2 and doxorubicin (as positive control drug) revealed cytotoxic effects towards the 18 tested cancer cell lines. The IC50 values ranged from 6.1 μg/mL (against CCRF-CEM cells) to 44.2 μg/mL (against BRAF-V600E homozygous mutant melanoma cells) for PSCb; from 4.3 μM (against CCRF-CEM cells) to 21.8 μM (against HCT116 p53-/-) for compound 2 and from 0.02 μM (against CCRF-CEM cells) to 123.0 μM (against CEM/ADR5000 cells) for doxorubicin. PCFb and compound 2 induced apoptosis in CCRF-CEM cells mediated by activation of caspase 3/7, 8 and 9, MMP alteration and increased ROS production. CONCLUSION: Piper capense is a source of potent cytotoxic botanicals and phytochemicals that could help to fight various types of cancer including multidrug resistance phenotypes. PCFb and compound 2 should further be explored to develop new drugs to fight malignancies.
- Date of acceptance
- 2021
- Autoren
- Armelle T Mbaveng
- Brice EN Wamba
- Gabin TM Bitchagno
- Simplice Beaudelaire Tankeo
- İlhami Çelik
- Brice CK Atontsa
- Antoine H Nkuété Lonfouo
- Victor Kuete
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/33529639
- DOI
- 10.1016/j.jep.2021.113884
- eISSN
- 1872-7573
- Zeitschrift
- J Ethnopharmacol
- Schlüsselwörter
- Apoptosis
- Cancer
- Cytotoxicity
- Multi-drug resistance
- Phytotherapy
- Piperaceae
- Animals
- Antineoplastic Agents, Phytogenic
- Apoptosis
- Caspases
- Cell Cycle
- Cell Line, Tumor
- Doxorubicin
- Drug Resistance, Neoplasm
- Fruit
- Humans
- Hydrogen Peroxide
- Lignans
- Membrane Potential, Mitochondrial
- Phytochemicals
- Piper
- Plant Extracts
- Reactive Oxygen Species
- Valinomycin
- Sprache
- eng
- Country
- Ireland
- Paginierung
- 113884
- PII
- S0378-8741(21)00110-0
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2021
- Titel
- Bioactivity of fractions and constituents of Piper capense fruits towards a broad panel of cancer cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 271
Data source: PubMed
- Beziehungen:
- Property of