Botanicals and phytochemicals from the bark of Hypericum roeperianum (Hypericaceae) had strong antibacterial activity and showed synergistic effects with antibiotics against multidrug-resistant bacteria expressing active efflux pumps

Publication type:
Journal article
Metadata:
Autoren
  • Olive Monique F Demgne
  • Francois Damen
  • Aime G Fankam
  • Michel-Gael F Guefack
  • Brice EN Wamba
  • Paul Nayim
  • Armelle T Mbaveng
  • Gabin TM Bitchagno
  • Leon Azefack Tapondjou
  • Veronique B Penlap
  • Pierre Tane
  • Thomas Efferth
  • Victor Kuete
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000663574500002&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.jep.2021.114257
eISSN
1872-7573
Externe Identifier
  • Clarivate Analytics Document Solution ID: SV1GU
  • PubMed Identifier: 34062249
ISSN
0378-8741
Zeitschrift
JOURNAL OF ETHNOPHARMACOLOGY
Schlüsselwörter
  • Antibacterial activity
  • 8
  • 8-bis(dihydroconiferyl)diferulate
  • Hypericum roeperianum
  • Hypericaceae
  • Multidrug resistance
  • Synergism
Artikelnummer
ARTN 114257
Datum der Veröffentlichung
2021
Status
Published
Titel
Botanicals and phytochemicals from the bark of Hypericum roeperianum (Hypericaceae) had strong antibacterial activity and showed synergistic effects with antibiotics against multidrug-resistant bacteria expressing active efflux pumps
Sub types
  • Article
Ausgabe der Zeitschrift
277

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Olive Monique F Demgne
  • Francois Damen
  • Aimé G Fankam
  • Michel-Gael F Guefack
  • Brice EN Wamba
  • Paul Nayim
  • Armelle T Mbaveng
  • Gabin TM Bitchagno
  • Léon Azefack Tapondjou
  • Veronique B Penlap
  • Pierre Tane
  • Thomas Efferth
  • Victor Kuete
DOI
10.1016/j.jep.2021.114257
ISSN
0378-8741
Zeitschrift
Journal of Ethnopharmacology
Sprache
en
Artikelnummer
114257
Paginierung
114257 - 114257
Datum der Veröffentlichung
2021
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.jep.2021.114257
Datum der Datenerfassung
2023
Titel
Botanicals and phytochemicals from the bark of Hypericum roeperianum (Hypericaceae) had strong antibacterial activity and showed synergistic effects with antibiotics against multidrug-resistant bacteria expressing active efflux pumps
Ausgabe der Zeitschrift
277

Data source: Crossref

Abstract
<h4>Ethnopharmacological relevance</h4>Infections due to multidrug-resistant (MDR) bacteria constitute a real problem in the public health worldwide. Hypericum roeperianum Schimp. ex A. Rich (Hypericaceae) is used traditionally for treatment of various ailments such as abdominal pains, constipation, diarrhea, indigestion, nausea, and bacterial diseases.<h4>Aim of the study</h4>This study was aimed at investigating the antibacterial and antibiotic-modifying activity of the crude methanol extracts (HRB), ethyl-acetate soluble fraction (HRBa), residual material (HRBb), and 11 compounds from the bark of Hypericum roeperianum against multi-drug resistant (MDR) bacteria expressing active efflux pumps.<h4>Materials and methods</h4>The antibacterial activity, the efflux pump effect using the efflux pump inhibitor (EPI), phenylalanine-arginine-ß-naphthylamide (PAβN), as well as the antibiotic-modifying activity of samples were determined using the broth micro-dilution method. Spectrophotometric methods were used to evaluate the effects of HRB and 8,8-bis(dihydroconiferyl) diferulate (11) on bacterial growth, and bacterial membrane damage, whereas follow-up of the acidification of the bacterial culture was used to study their effects on bacteria proton-ATPase pumps.<h4>Results</h4>The crude extract (HRB), HRBa, and HRBb had selective antibacterial activity with MICs ranging from 16 to 512 μg/mL. Phytochemical 11 displayed the best antibacterial activity (0.5 ≤ MIC ≤ 2 μg/mL). The activity of HRB and 11 in the presence of EPI significantly increased on the tested bacteria strains (up to 32-fold). The activity of cloxacillin (CLO), doxycycline (DOX), and tetracycline (TET), was considerably improved (up to 64-fold) towards the multidrug-resistant Enterobacter aerogenes EA-CM64 strain. The crude extract (HRB) and 11 induced the leakage of bacterial intracellular components and inhibited the proton-ATPase pumps.<h4>Conclusions</h4>The crude extract (HRB) and 8,8-bis(dihydroconiferyl)diferulate from the bark of Hypericum roeperianum are good antibacterial candidates that deserve further investigations to achieve antibacterial drugs to fight infections involving MDR bacteria.
Addresses
  • Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon. Electronic address: princessdemleur@yahoo.fr.
Autoren
  • Olive Monique F Demgne
  • Francois Damen
  • Aimé G Fankam
  • Michel-Gael F Guefack
  • Brice EN Wamba
  • Paul Nayim
  • Armelle T Mbaveng
  • Gabin TM Bitchagno
  • Léon Azefack Tapondjou
  • Veronique B Penlap
  • Pierre Tane
  • Thomas Efferth
  • Victor Kuete
DOI
10.1016/j.jep.2021.114257
eISSN
1872-7573
Externe Identifier
  • PubMed Identifier: 34062249
Funding acknowledgements
  • Alexander von Humboldt-Stiftung: 3.4 - 8151/Kuete
  • Alexander von Humboldt-Stiftung: 3.4 - 8151 /18026
  • Alexander von Humboldt-Stiftung: GA-Nr.19014
Open access
false
ISSN
0378-8741
Zeitschrift
Journal of ethnopharmacology
Schlüsselwörter
  • Bacteria
  • Hypericum
  • Plant Bark
  • Membrane Transport Proteins
  • Plant Extracts
  • Anti-Bacterial Agents
  • Microbial Sensitivity Tests
  • Drug Resistance, Multiple, Bacterial
  • Drug Synergism
  • Phytochemicals
Sprache
eng
Medium
Print-Electronic
Online publication date
2021
Paginierung
114257
Datum der Veröffentlichung
2021
Status
Published
Datum der Datenerfassung
2021
Titel
Botanicals and phytochemicals from the bark of Hypericum roeperianum (Hypericaceae) had strong antibacterial activity and showed synergistic effects with antibiotics against multidrug-resistant bacteria expressing active efflux pumps.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
277

Data source: Europe PubMed Central

Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Infections due to multidrug-resistant (MDR) bacteria constitute a real problem in the public health worldwide. Hypericum roeperianum Schimp. ex A. Rich (Hypericaceae) is used traditionally for treatment of various ailments such as abdominal pains, constipation, diarrhea, indigestion, nausea, and bacterial diseases. AIM OF THE STUDY: This study was aimed at investigating the antibacterial and antibiotic-modifying activity of the crude methanol extracts (HRB), ethyl-acetate soluble fraction (HRBa), residual material (HRBb), and 11 compounds from the bark of Hypericum roeperianum against multi-drug resistant (MDR) bacteria expressing active efflux pumps. MATERIALS AND METHODS: The antibacterial activity, the efflux pump effect using the efflux pump inhibitor (EPI), phenylalanine-arginine-ß-naphthylamide (PAβN), as well as the antibiotic-modifying activity of samples were determined using the broth micro-dilution method. Spectrophotometric methods were used to evaluate the effects of HRB and 8,8-bis(dihydroconiferyl) diferulate (11) on bacterial growth, and bacterial membrane damage, whereas follow-up of the acidification of the bacterial culture was used to study their effects on bacteria proton-ATPase pumps. RESULTS: The crude extract (HRB), HRBa, and HRBb had selective antibacterial activity with MICs ranging from 16 to 512 μg/mL. Phytochemical 11 displayed the best antibacterial activity (0.5 ≤ MIC ≤ 2 μg/mL). The activity of HRB and 11 in the presence of EPI significantly increased on the tested bacteria strains (up to 32-fold). The activity of cloxacillin (CLO), doxycycline (DOX), and tetracycline (TET), was considerably improved (up to 64-fold) towards the multidrug-resistant Enterobacter aerogenes EA-CM64 strain. The crude extract (HRB) and 11 induced the leakage of bacterial intracellular components and inhibited the proton-ATPase pumps. CONCLUSIONS: The crude extract (HRB) and 8,8-bis(dihydroconiferyl)diferulate from the bark of Hypericum roeperianum are good antibacterial candidates that deserve further investigations to achieve antibacterial drugs to fight infections involving MDR bacteria.
Date of acceptance
2021
Autoren
  • Olive Monique F Demgne
  • Francois Damen
  • Aimé G Fankam
  • Michel-Gael F Guefack
  • Brice EN Wamba
  • Paul Nayim
  • Armelle T Mbaveng
  • Gabin TM Bitchagno
  • Léon Azefack Tapondjou
  • Veronique B Penlap
  • Pierre Tane
  • Thomas Efferth
  • Victor Kuete
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/34062249
DOI
10.1016/j.jep.2021.114257
eISSN
1872-7573
Zeitschrift
J Ethnopharmacol
Schlüsselwörter
  • 8,8-bis(dihydroconiferyl)diferulate
  • Antibacterial activity
  • Hypericaceae
  • Hypericum roeperianum
  • Multidrug resistance
  • Synergism
  • Anti-Bacterial Agents
  • Bacteria
  • Drug Resistance, Multiple, Bacterial
  • Drug Synergism
  • Hypericum
  • Membrane Transport Proteins
  • Microbial Sensitivity Tests
  • Phytochemicals
  • Plant Bark
  • Plant Extracts
Sprache
eng
Country
Ireland
Paginierung
114257
PII
S0378-8741(21)00484-0
Datum der Veröffentlichung
2021
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2021
Titel
Botanicals and phytochemicals from the bark of Hypericum roeperianum (Hypericaceae) had strong antibacterial activity and showed synergistic effects with antibiotics against multidrug-resistant bacteria expressing active efflux pumps.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
277

Data source: PubMed

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