Anti-inflammatory and tight junction protective activity of the herbal preparation STW 5-II on mouse intestinal organoids
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Mohamed Elbadawi
- Ramy M Ammar
- Heba Aziz-Kalbhenn
- Sabine Rabini
- Sabine M Klauck
- Mona Dawood
- Mohamed EM Saeed
- Christopher J Kampf
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000661732400009&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.phymed.2021.153589
- eISSN
- 1618-095X
- Externe Identifier
- Clarivate Analytics Document Solution ID: SS4OC
- PubMed Identifier: 34111617
- ISSN
- 0944-7113
- Zeitschrift
- PHYTOMEDICINE
- Schlüsselwörter
- Irritable bowel syndrome
- Mouse intestinal organoids
- Tight junctions
- Intestinal inflammation
- Proinflammatory cytokines
- Artikelnummer
- ARTN 153589
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Titel
- Anti-inflammatory and tight junction protective activity of the herbal preparation STW 5-II on mouse intestinal organoids
- Sub types
- Article
- Ausgabe der Zeitschrift
- 88
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Mohamed Elbadawi
- Ramy M Ammar
- Heba Aziz-Kalbhenn
- Sabine Rabini
- Sabine M Klauck
- Mona Dawood
- Mohamed EM Saeed
- Christopher J Kampf
- Thomas Efferth
- DOI
- 10.1016/j.phymed.2021.153589
- ISSN
- 0944-7113
- Zeitschrift
- Phytomedicine
- Sprache
- en
- Artikelnummer
- 153589
- Paginierung
- 153589 - 153589
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.phymed.2021.153589
- Datum der Datenerfassung
- 2021
- Titel
- Anti-inflammatory and tight junction protective activity of the herbal preparation STW 5-II on mouse intestinal organoids
- Ausgabe der Zeitschrift
- 88
Data source: Crossref
- Abstract
- <h4>Background</h4>Irritable bowel syndrome (IBS) is a functional bowel disorder, in which recurrent abdominal pain is associated with defecation or a change in bowel habits. STW 5-II is a combination of six medicinal herbs with a clinically proven efficacy in managing IBS.<h4>Aim</h4>This study aims to establish an in vitro IBS model using mouse intestinal organoids and to explore the anti-inflammatory and tight junction protective activities of the multi-herbal preparation STW 5-II.<h4>Methods</h4>Intestinal organoids were cultured in 1:1 Matrigel™ and medium domes. Inflammation and tight junction disruption were induced by a cocktail of cytokines (TNFα, IFNγ, IL-1β, IL-6) and bacterial proteins (LPS, flagellin). Organoids were treated with different concentrations of STW 5-II, and its multi-target activity was assessed using microarray analyses, RT-qPCR, immunofluorescence, western blot, immunohistochemistry, and a FITC permeability assay. In addition, we analyzed the expression of pNF-κB, pSTAT1, iNOS and ZO-1. In silico analyses were conducted to predict and identify the active components that may be responsible in mediating the multi-target anti-inflammatory activity of STW 5-II.<h4>Results</h4>An organoid based IBS model was successfully established. STW 5-II effectively reduced the cytokines-induced overexpression of the pro-inflammatory mediators pNF-κB, pSTAT1 and iNOS. Moreover, STW 5-II attenuated cytokine-mediated downregulation of the tight junction protein, ZO-1. This finding was confirmed by a FITC permeability assay. In silico analyses revealed a promising inhibitory activity of some isolated compounds from STW 5-II against NF-κB, STAT1 and iNOS.<h4>Conclusion</h4>STW 5-II possesses multiple anti-inflammatory as well as tight junction protective activities that could explain its clinically proven efficacy in managing IBS symptoms.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmaceutical and Biomedical Sciences, Johannes Gutenberg University, Mainz, Germany.
- Autoren
- Mohamed Elbadawi
- Ramy M Ammar
- Heba Aziz-Kalbhenn
- Sabine Rabini
- Sabine M Klauck
- Mona Dawood
- Mohamed EM Saeed
- Christopher J Kampf
- Thomas Efferth
- DOI
- 10.1016/j.phymed.2021.153589
- eISSN
- 1618-095X
- Externe Identifier
- PubMed Identifier: 34111617
- Funding acknowledgements
- Bayer AG:
- Open access
- false
- ISSN
- 0944-7113
- Zeitschrift
- Phytomedicine : international journal of phytotherapy and phytopharmacology
- Schlüsselwörter
- Intestines
- Intestinal Mucosa
- Organoids
- Tight Junctions
- Animals
- Mice
- Irritable Bowel Syndrome
- Disease Models, Animal
- NF-kappa B
- Anti-Inflammatory Agents
- Inflammation Mediators
- Plant Extracts
- Organ Culture Techniques
- Gene Expression Regulation
- Computer Simulation
- STAT1 Transcription Factor
- Zonula Occludens-1 Protein
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2021
- Paginierung
- 153589
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Publisher licence
- CC BY-NC-ND
- Datum der Datenerfassung
- 2021
- Titel
- Anti-inflammatory and tight junction protective activity of the herbal preparation STW 5-II on mouse intestinal organoids.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 88
Data source: Europe PubMed Central
- Abstract
- BACKGROUND: Irritable bowel syndrome (IBS) is a functional bowel disorder, in which recurrent abdominal pain is associated with defecation or a change in bowel habits. STW 5-II is a combination of six medicinal herbs with a clinically proven efficacy in managing IBS. AIM: This study aims to establish an in vitro IBS model using mouse intestinal organoids and to explore the anti-inflammatory and tight junction protective activities of the multi-herbal preparation STW 5-II. METHODS: Intestinal organoids were cultured in 1:1 Matrigel™ and medium domes. Inflammation and tight junction disruption were induced by a cocktail of cytokines (TNFα, IFNγ, IL-1β, IL-6) and bacterial proteins (LPS, flagellin). Organoids were treated with different concentrations of STW 5-II, and its multi-target activity was assessed using microarray analyses, RT-qPCR, immunofluorescence, western blot, immunohistochemistry, and a FITC permeability assay. In addition, we analyzed the expression of pNF-κB, pSTAT1, iNOS and ZO-1. In silico analyses were conducted to predict and identify the active components that may be responsible in mediating the multi-target anti-inflammatory activity of STW 5-II. RESULTS: An organoid based IBS model was successfully established. STW 5-II effectively reduced the cytokines-induced overexpression of the pro-inflammatory mediators pNF-κB, pSTAT1 and iNOS. Moreover, STW 5-II attenuated cytokine-mediated downregulation of the tight junction protein, ZO-1. This finding was confirmed by a FITC permeability assay. In silico analyses revealed a promising inhibitory activity of some isolated compounds from STW 5-II against NF-κB, STAT1 and iNOS. CONCLUSION: STW 5-II possesses multiple anti-inflammatory as well as tight junction protective activities that could explain its clinically proven efficacy in managing IBS symptoms.
- Date of acceptance
- 2021
- Autoren
- Mohamed Elbadawi
- Ramy M Ammar
- Heba Aziz-Kalbhenn
- Sabine Rabini
- Sabine M Klauck
- Mona Dawood
- Mohamed EM Saeed
- Christopher J Kampf
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/34111617
- DOI
- 10.1016/j.phymed.2021.153589
- eISSN
- 1618-095X
- Zeitschrift
- Phytomedicine
- Schlüsselwörter
- Intestinal inflammation
- Irritable bowel syndrome
- Mouse intestinal organoids
- Proinflammatory cytokines
- Tight junctions
- Animals
- Anti-Inflammatory Agents
- Computer Simulation
- Disease Models, Animal
- Gene Expression Regulation
- Inflammation Mediators
- Intestinal Mucosa
- Intestines
- Irritable Bowel Syndrome
- Mice
- NF-kappa B
- Organ Culture Techniques
- Organoids
- Plant Extracts
- STAT1 Transcription Factor
- Tight Junctions
- Zonula Occludens-1 Protein
- Sprache
- eng
- Country
- Germany
- Paginierung
- 153589
- PII
- S0944-7113(21)00131-8
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2021
- Titel
- Anti-inflammatory and tight junction protective activity of the herbal preparation STW 5-II on mouse intestinal organoids.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 88
Data source: PubMed
- Beziehungen:
- Property of